pCJH9_pETcc2-FruASc-E2Aa vector (V015717)

LOCUS       V015717                 4351 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V015717
VERSION     V015717
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4351)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..4351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..891
                     /gene="fda"
                     /label="Fructose-bisphosphate aldolase class 1"
                     /note="Fructose-bisphosphate aldolase class 1 from
                     Staphylococcus carnosus (strain TM300). Accession#: Q07159"
     terminator      1852..1899
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(2265..2293)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        2406..2510
                     /label="AmpR promoter"
     CDS             2511..3368
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3542..4130
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4210..4228
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4260..4282
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4301..4318
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4328..4345
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"