LOCUS       V015717                 4351 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V015717
VERSION     V015717
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4351)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..4351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..891
                     /gene="fda"
                     /label="Fructose-bisphosphate aldolase class 1"
                     /note="Fructose-bisphosphate aldolase class 1 from
                     Staphylococcus carnosus (strain TM300). Accession#: Q07159"
     terminator      1852..1899
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(2265..2293)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        2406..2510
                     /label="AmpR promoter"
     CDS             2511..3368
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3542..4130
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4210..4228
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4260..4282
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4301..4318
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4328..4345
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
ORIGIN
        1 catatgaatc aagagcagtt cgacaaaatc aaaaacggca aaggttttat tgcagcactg
       61 gatcagagcg gtggtagcac cccgaaagca ctgaaagatt atggtgttga agaaaacgag
      121 tatagcaacg acgaagaaat gtttaacctg gtgcatgata tgcgtacccg tattattacc
      181 agtccggcat ttaatggcga aaaaattctg ggtgcaatcc tgtttgaaca gaccatggat
      241 cgtgaagtgg aaggtaaata taccggtagc tatctggcag ataaaggtat tgttccgttt
      301 ctgaaagtgg ataaaggtct ggccgaagag gcagatggtg ttcagctgat gaaaccgatt
      361 ccggatctgg ataaactgct ggatcgtgca aatgaacgtg gtatttttgg caccaaaatg
      421 cgcagcaata ttctggaaaa taacaaagag gccatcgaaa aagttgtgaa acagcagttt
      481 gaagtggcca aagaaattat tgccgcaggt ctggttccga ttattgaacc ggaagttaac
      541 attaacgcca aagataaaga agcgattgaa gcaaatctgg cggaagcaat taaagcggaa
      601 ctggacaatc tgaaaaaaga ccagtatgtg atgctgaaac tgaccattcc gaccaaagtt
      661 aatgcatata gcgaactgat tgaacacccg caggttattc gtgttgttgc actgagtggt
      721 ggttatagcc gtgatgaagc aaacaaaatt ctgaaacaga acgatggtct gattgcaagc
      781 tttagccgtg cactggttag cgatctgaat gcacagcaga gtgatgcaga atttaacgaa
      841 aaactgcaag aagccatcga taccattttt gatgccagcg ttaataaagc aggtagcatg
      901 ccgctggacc cggttatcca gcaggttctg gatcagctga atcgtatgcc agcaccggat
      961 tataaacatc tgagcgcaca gcagttccgt agccagcaga gcctgtttcc gcctgttaaa
     1021 aaagaaccgg tggccgaagt tcgtgaattt gacatggatc tgcctggtcg taccctgaaa
     1081 gttcgtatgt atcgtccgga aggtgtggaa ccgccttatc cggcactggt ttattatcat
     1141 ggtggtggtt gggtggttgg tgatctggaa acccatgatc cggtttgtcg tgttctggca
     1201 aaagatggtc gtgcagttgt ttttagcgtt gattatcgtc tggcaccgga acataaattt
     1261 ccggcagcag tggaagatgc ctatgatgcc ctgcagtgga ttgcagaacg tgcagccgat
     1321 tttcatctgg accctgcacg tattgcagtt ggtggtgata gtgccggtgg taatctggca
     1381 gcagttacca gcattctggc gaaagaacgt ggtggtcctg cactggcatt tcaactgctg
     1441 atttatccga gcaccggtta tgatccggca catccgcctg caagcattga agaaaatgca
     1501 gaaggttatc tgctgaccgg tggtatgatg ctgtggtttc gtgatcagta tctgaatagc
     1561 ctggaagaac tgacccatcc gtggtttagt ccggttctgt atccggatct gagcggtctg
     1621 cctccggcat atattgcaac cgcacagtat gatccgctgc gtgatgttgg taaactgtat
     1681 gcagaagcac tgaataaagc cggtgttaaa gtggaaatcg aaaactttga ggatctgatt
     1741 catggctttg cccagtttta tagcctgagt ccgggtgcaa ccaaagcact ggttcgtatt
     1801 gccgaaaaac tgcgtgacgc actggcctaa taaggatccg ctgagcaata actagcataa
     1861 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     1921 ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag
     1981 cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc
     2041 gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct
     2101 gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc
     2161 tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca
     2221 cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat
     2281 gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     2341 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     2401 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2461 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     2521 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     2581 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     2641 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     2701 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     2761 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     2821 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     2881 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     2941 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3001 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa
     3061 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     3121 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     3181 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3241 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3301 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3361 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     3421 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     3481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3661 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     3721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg gatctcgatc
     4201 ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct agaaataatt
     4261 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
     4321 cagcggcctg gtgccgcgcg gcagccatat g
//
