pAS19_yPSAT1_k vector (V015714)

LOCUS       V015714                 6097 bp    DNA     circular SYN 01-MAR-2020
DEFINITION  Exported.
ACCESSION   V015714
VERSION     V015714
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Cromie G.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2019) Dudley Lab, PNRI, 720 Broadway, Seattle, WA
            98027, USA
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (07-NOV-2019) Dudley Lab, PNRI, 720 Broadway, Seattle, WA 98027,
            USA"
FEATURES             Location/Qualifiers
     source          1..6097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(719..823)
                     /label="AmpR promoter"
     primer_bind     1297..1313
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1439..2548
                     /gene="PSAT1"
                     /label="Phosphoserine aminotransferase"
                     /note="Phosphoserine aminotransferase from Homo sapiens.
                     Accession#: Q9Y617"
     gene            3105..4461
                     /label="kanMX"
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     primer_bind     complement(4794..4810)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4818..4834)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4842..4872)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4887..4908)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5196..5784)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(join(5958..6097,1..718))
                     /label="AmpR"
                     /note="beta-lactamase"