LOCUS       V015714                 6097 bp    DNA     circular SYN 01-MAR-2020
DEFINITION  Exported.
ACCESSION   V015714
VERSION     V015714
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Cromie G.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2019) Dudley Lab, PNRI, 720 Broadway, Seattle, WA
            98027, USA
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (07-NOV-2019) Dudley Lab, PNRI, 720 Broadway, Seattle, WA 98027,
            USA"
FEATURES             Location/Qualifiers
     source          1..6097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(719..823)
                     /label="AmpR promoter"
     primer_bind     1297..1313
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1439..2548
                     /gene="PSAT1"
                     /label="Phosphoserine aminotransferase"
                     /note="Phosphoserine aminotransferase from Homo sapiens.
                     Accession#: Q9Y617"
     gene            3105..4461
                     /label="kanMX"
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     primer_bind     complement(4794..4810)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4818..4834)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4842..4872)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4887..4908)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5196..5784)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(join(5958..6097,1..718))
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
      901 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
      961 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     1021 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     1081 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     1141 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     1201 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     1261 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg
     1321 agctcggtac ccggggctct tcgacaaaga cattaaggag cctttgaaat tacttaattg
     1381 aacaacacag gtctaattag ttgatcaatc atcgattaac cattagtgat aagaaacaat
     1441 ggacgcgcca agacaggttg tgaatttcgg acctgggcca gctaaattgc ctcactctgt
     1501 tttgttggaa atccagaaag agttattaga ttataaaggt gtaggtatat cagtcttgga
     1561 aatgtctcac cgttcgtctg atttcgccaa aataattaat aacaccgaga acttagttag
     1621 ggaactgcta gccgttcctg acaactataa agtgattttt ttgcaaggtg gtggctgcgg
     1681 tcaattttct gccgttcctt taaatttgat aggtttaaaa gcgggtagat gtgccgacta
     1741 tgtcgtcacc ggtgcgtggt ctgctaaagc cgctgaagag gcaaagaaat tcggtactat
     1801 caacattgtt catccaaagt tgggctctta cactaaaatt ccggatccat ccacatggaa
     1861 tttaaatccc gatgcatcat acgtgtacta ctgtgctaat gaaactgttc atggtgttga
     1921 atttgacttt attccagatg ttaagggtgc tgttttggtt tgcgacatgt ccagcaactt
     1981 cttgtctaag ccagttgacg tttctaaatt cggtgtcatc tttgcaggcg cccaaaaaaa
     2041 cgttggttct gcaggcgtaa ccgtggttat tgtcagagat gacttacttg gttttgcgtt
     2101 aagagaatgc ccttctgttt tagagtataa ggtgcaggcc ggtaactcat cactgtataa
     2161 tacaccacca tgcttctcta tatatgttat gggtctcgtg ttagaatgga ttaagaataa
     2221 tggtggtgca gctgcgatgg agaaattgtc ttctattaag tctcagacga tatatgagat
     2281 aatagataac tcacagggtt tttacgtttg cccagtggaa ccacagaata gatcaaaaat
     2341 gaatatccca ttccgtatag gtaacgcgaa gggcgatgac gcccttgaaa agaggtttct
     2401 agataaggca cttgagttaa atatgctgtc tttgaaagga catcgttcag ttggtgggat
     2461 cagagcctcc ttgtataacg cggtgacgat cgaagacgta caaaagttgg ccgcttttat
     2521 gaagaaattt ttggagatgc accagttata atatcagcgg aatatgcctg taatttggtt
     2581 caaagaaaaa ctttttcaag tcatatgtaa gttttaaata aatcagttta cgaacatgca
     2641 caagaataac ttctcttctt tttcttgaaa taaaggccga attatacgat atgatatgag
     2701 acgttctaat tacaatacga taccgtgaga taaaaatagc gatatcctcg tactcaaaga
     2761 agtctaattg ttttagcccg ttgatcaaaa aaataaaatg tatttttatt tttttgtcat
     2821 ccagctcaag atcgtaatat tggcaattaa caaccgagcg aggcacaggg caaagagaat
     2881 gtgggtgaga ctgaactatc tctcccaata tattatactt ttttttgtcg cgtctaccaa
     2941 ttaaatgaaa acataattgg gaatactttc ctcttcttat attttatctt aactcgtttc
     3001 cgatcttctg atatcgtacg ctgcaggtcg acggatcccc gggttaatta aggcgcgcca
     3061 gatctgttta gcttgccttg tccccgccgg gtcacccggc cagcgacatg gaggcccaga
     3121 ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt
     3181 acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca
     3241 cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc
     3301 tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg
     3361 atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt
     3421 ctcacatcac atccgaacat aaacaaccat gggtaaggaa aagactcacg tttcgaggcc
     3481 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     3541 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
     3601 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     3661 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     3721 tgcatggtta ctcaccactg cgatccccgg caaaacagca ttccaggtat tagaagaata
     3781 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     3841 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     3901 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     3961 gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt
     4021 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     4081 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     4141 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     4201 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     4261 agtactgaca ataaaaagat tcttgttttc aagaacttgt catttgtata gtttttttat
     4321 attgtagttg ttctatttta atcaaatgtt agcgtgattt atattttttt tcgcctcgac
     4381 atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa tatcatgcgt caatcgtatg
     4441 tgaatgctgg tcgctatact gctgtcgatt cgatactaac gccgccatcc agtgtcgaaa
     4501 acgagctcga attcatcgat aagaagtatc tcttttcaag aggattactt ttcattgaaa
     4561 acttttggac gggcaaaaaa aaaaaaagcg ccagcaggaa gaaaaaagga atctagtaaa
     4621 aaataaaaat taggtttata aagtagtaag tgaagtgcaa gagggagcgt tattggacga
     4681 tcatgttgtg atcggatccc gtttattggt ctttgattca atttaaaaga aaagaaatgt
     4741 acaggttaaa cttcaacata cgtatgcagg catgcaagct tggcgtaatc atggtcatag
     4801 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4861 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4921 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     4981 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5041 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5101 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5161 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5221 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5281 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5341 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5401 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5461 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5521 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5581 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5641 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5701 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5761 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5821 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5881 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5941 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6001 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6061 ttaccatctg gccccagtgc tgcaatgata ccgcgag
//
