pLT_208 vector (V015711)

LOCUS       V015711                 9009 bp    DNA     circular SYN 27-AUG-2019
DEFINITION  Exported.
ACCESSION   V015711
VERSION     V015711
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Tian L, Conway PM, Cervenka ND, Cui J, Maloney M, Olson DG, Lynd LR.
  TITLE     Metabolic engineering of Clostridium thermocellum for n-butanol
            production from cellulose
  JOURNAL   Biotechnol Biofuels 12, 186 (2019)
   PUBMED   31367231
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2019) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Dr, Hanover, New Hampshire 03755, United
            States
REFERENCE   3  (bases 1 to 9009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 12, 186 (2019)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-FEB-2019) Thayer School of Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, New Hampshire 03755, United States"
FEATURES             Location/Qualifiers
     source          1..9009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(141..1088)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(1136..1358)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             7032..7049
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             7272..8072
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             8318..8965
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"