LOCUS       V015711                 9009 bp    DNA     circular SYN 27-AUG-2019
DEFINITION  Exported.
ACCESSION   V015711
VERSION     V015711
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Tian L, Conway PM, Cervenka ND, Cui J, Maloney M, Olson DG, Lynd LR.
  TITLE     Metabolic engineering of Clostridium thermocellum for n-butanol
            production from cellulose
  JOURNAL   Biotechnol Biofuels 12, 186 (2019)
   PUBMED   31367231
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2019) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Dr, Hanover, New Hampshire 03755, United
            States
REFERENCE   3  (bases 1 to 9009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 12, 186 (2019)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-FEB-2019) Thayer School of Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, New Hampshire 03755, United States"
FEATURES             Location/Qualifiers
     source          1..9009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(141..1088)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(1136..1358)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             7032..7049
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             7272..8072
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             8318..8965
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
ORIGIN
        1 aatgtcacta gggctcgcct ttgggaagtt tgaagcatat ggacagtttt ccctttgata
       61 tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata
      121 caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc tagtgtggtt
      181 cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact ttgcatgtca
      241 ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt
      301 gtttttctta gtccgttatg taggtaggaa tctgatgtaa tggttgttgg tattttgtca
      361 ccattcattt ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt
      421 tcaacttgga aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata
      481 ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta
      541 aactcatggt agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct
      601 atatttgcct tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc
      661 atagagtatt tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt
      721 aactggaaaa gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag
      781 aacttggcat agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt
      841 tccacagttc tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc
      901 ctactgatgt tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc
      961 cttgtagggt tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt
     1021 tcatgctccg ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat
     1081 tcagacatac atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct
     1141 agtcaatgat aattactagt ccttttcctt tgagttgtgg gtatctgtaa attctgctag
     1201 acctttgctg gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg
     1261 tgtgtttttt ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa
     1321 aagataaaaa gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag
     1381 tattacaaaa ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct
     1441 aaaggcttaa gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc
     1501 gaccatcagg cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc
     1561 tctggcagtg aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa
     1621 actacccata atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct
     1681 gcggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg
     1741 gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat
     1801 ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg
     1861 tccaatgatc gaagtthagg ctggtggtac caccgtgagc attctggaca ggttcaacag
     1921 cctgaaaggg caaaaccccc cttgaaaggg tttaaataca cacgtccttt ttgtcgtttt
     1981 ttgtcggttt aaatatttat gcaggggatg atagggaatg ggaaagccgt ccataatcaa
     2041 gcaagtactc aatgaattcg aaaagcagat aaggttcggg gaaagtaagc atgaagcaaa
     2101 gagagaagag cgggagagat gtgaagttac tggagagacg tggaatcctg ctcgtgtgga
     2161 aggcattttt agtttctcaa cctacaggga gtacgttaag gaggcgttag aatttgcgaa
     2221 ttgggctcgt actgaaaagg ggtgtaagga tttagaacaa gcacgggcct atgtgtctga
     2281 atatttgcag tcgcatatag acaaagggta tagcgcgtgg actgttaaga aagaagcagc
     2341 agccctggcg aaactgtatc attgtcgtac aactgacttt aaagtagagc ttcccgcaag
     2401 acacagggaa gagattgaga gaagcagggg atacaaagac cacgataggg agtttagcaa
     2461 agagaggaat agagacatta tcatcttttc aaaagctact gggctgagaa gaagggaatt
     2521 ggaaagagtg agttctcggg atatctttcg tgggcctgac ggaagattat atgtgcacgt
     2581 gagcaacggc aagggcggta gagaaaggga tgttcatgtt ttgcagaaat acgagagaga
     2641 ggttgagagg atagtcagag agcgggaagg aagagacagg ctgtttgaca gggtccccat
     2701 aaggatggac gtacacagct ataggaggga atatgcaaga gagcgttaca gagaagttga
     2761 gcgtgagata agtcgtgaga gaaagctttt cgacagagtt gaggatctcg ttcgtagtag
     2821 gcttacaagg ctctatcctg acaggtttag agaaattggc gaaagacaac ttactcgtga
     2881 actcacaaga gctgatgggc tttatcatcg cagtgatggt agggagtttg accgcctggc
     2941 attgtgggaa gtttcaaacg acttgggaca taatcgaatt gacgttgttg caagacacta
     3001 tctggattaa gcgaataaag gctcaagaaa gtggataaaa aaacaggggg tatattgata
     3061 tatcccccct gttttttgtg cgtctacagg accttatttg cgtttcaagg ctattcttgt
     3121 ggggtagtgc agtaaaaaag gttgcctttg tatcttgcct gtagaatctt gccgttttca
     3181 aaaacaaatt caacgttgca cttatcagca agtttcttga ttattccctg ctcaaattcg
     3241 tcgaacctgt acccttccat tgctcttgct gtgaaataac ccagaaggta cattgcttct
     3301 tcacgtgtaa actcaattgt tagctttttc actctctttt acccccttta cgagactgag
     3361 atattctttg tacaatctgt caatacgttc tgtatcgtca ggttgtacgt ttttgagttt
     3421 ttcgtagaga ttatgaaaca acaggttctc tgctgttcca atagggtatt tctttctttc
     3481 cgcgtggact agccttttca gttggtttag ctttacgttc cggatcttgc tcagtaggtc
     3541 ttcgacgata ttttcaatct gttttgcttc gtcttcttcg atgtagaagc atagttgttt
     3601 gatttcaagt agcctctgaa taagttcctg ctttgttaac attccttgtc acccttcccc
     3661 ttttcagtct tttttatttt gtcgtattct gattcgaact ttctcagttg gtcagctctg
     3721 ccgtaacgta tgcgtcgagg atttagcatg aatcgtccac ggcctatttt aatcaacaaa
     3781 tctttctcga ttagcttttt gattgcaaga tggtattgct tttttgacat gtcgatgttt
     3841 cctttgatgt cgtattcgta tgaaagtgca aacgtgtagt tgttaaacga gattttgtgt
     3901 tgtacaatgt atgagagaat tcgcattgct gattttgcaa ggtctttatc ttccattatg
     3961 agtttgatag aaatgaatgg aagtttgaca aagtctgtgt cttcaaaatc atgtccgatc
     4021 agtatgaaag tttgtacttc gcctgtttca gggtcgacca tttttttgta ccctatcttc
     4081 ttcatttgaa tccccctttt tttacctttt tccaattcta aagccattat aatacactta
     4141 aagtaacttg tcaagtaact tcaggtgtaa aaaattacac ttcaggtgta ataaattaca
     4201 ctttccattc agtattttca aggctttgtg ggtaacttta ttcttatcta tgtatatatc
     4261 gcctgcgtta gcaggcttga aaatttccag ttaggataag caggaacaac ggtcgctgac
     4321 gctgaacact gacgaaatag ctgacgcccc aaagtccaca acagtgccaa accgataaca
     4381 aaaacatgct aacgcaaaca tagactaacg cacgactgac gtcgtgatgt gtgtgtgggc
     4441 ctacctacac acaaaaagaa ctaacaacag ctgactaacg tctgaagagc tctaacaaca
     4501 ctttgctaac gctgagctaa cggacagctc aacgttaaca cccgctaaaa aaattagagc
     4561 aggatttgag caagaggggc gctgcaaccc cctttgaaac cgctttcata acccgaaaag
     4621 ctcttcaccc accgcatcaa tattcatacc cgcttgcata attattcatc cctgattgta
     4681 ccacaaaacc cagaaaagtc ctgggaaaat catgaagatt cttccatttt ccaatttcca
     4741 ggaagcgaat tgaaattccc agggattttt tgcctttttc cagggaatga actggaattt
     4801 ccagcggttt ttccagggac ttaaccacac gaaaggtact ttccgccatc aaaggcttgc
     4861 aaacccatag gaaactactg gcaggcaaaa ccttccaccc agtagcaaat ctcttctacc
     4921 ttgttttgcg agcgttagca gggagctgtc cgttagcttt gtgttagcat ccttctgtta
     4981 gcctttggca cgttagtaga cttttgttag ttctctcttc ttgtttttat cttcattgtg
     5041 attgttttcc agaagactgc ttgacatttt gttattgtag tgttatgatc tatttcagaa
     5101 gcgaaaagta aaccgcggta gcgggttact ttttgcgtaa tatttggaat tgtcagtttg
     5161 gcacgggaaa tggaatcggg caggtatagg gaaaggcaat agccgtggac ggcgttccga
     5221 ttccgctttg tttgtgcgtt agcacatgag tcttgctgtt atcacgctct gctctgtggt
     5281 agtacaccgc aaggggtaca ccatttggag ttttgccgca tctgagagtt tagcagtttg
     5341 caaaacaaag ttcgcagtta gaaaagttag gcaaggggcg atgacgtgca cgtctgacac
     5401 gcttgacgtg caccgagccg agagtagagc gtgatgacat agaaaaagcc cagtagcagg
     5461 ttgtgcgtac cgtgacatgc tactgtggaa gacaagtaaa tagtcaaaaa acggtgcgct
     5521 tactgttcgt gggctcatgg gaatctcgag tctatcacat aatatgacca gctcataatt
     5581 tttatgaata aatttttgtc aaaaaaatat ttttatattt taattatctg tgatataatt
     5641 atcgtggaac tgttatataa agtctaaagg tatatatata agcattttca gaaaactgtt
     5701 atataatcgc ttaaccgctg ttgtataaca gtacatattt gtttatgctg tattataata
     5761 gtttgcttat atatccttag atttaaatat aaatttaaaa agagagggga aatacacgta
     5821 gagaccatga aattctttta aggaggtaaa aaatgaaaga agtagtaata gcaagtggcg
     5881 taagaacagc ggtcggaaaa tttggtggaa cccttttaaa cgttccagct gtggacctgg
     5941 gtgcggtggt aattaaagag gctttaaaaa gggcaaatgt aaaaccggaa gacgtttcag
     6001 aggttattat gggaaatgtt ttgcaggcag gattgggaca aaatccggca aggcaggctg
     6061 aaataaaggc aggcataccg caggaagttc ctgcaatgac agtgaatatg gtatgcggaa
     6121 gcggcttaag agctgtaaca ttggctgcac aggccgttat gctgggagac gcagatattg
     6181 tagttgcggg aggtatggaa aacatgtcca gggctcctta tattttaaat gatgcaagat
     6241 tcggatacgg catgaataac ggacagcttg ttgatgaaat ggtttatgac ggacttaccg
     6301 atgtttttta tcaatataat atgggaatta ctgcggaaaa tcttgcagaa aaatatggaa
     6361 taagcaggga agaacaggat gaatttgcat acaggagtca aaagctggct tcggaagcaa
     6421 tttcttccgg aagatttgaa gatgaaattg ttcccgttat agttccccaa aaaaagggag
     6481 aacctattga attcaaagtt gacgaacatg tgagatttgt gacaaccata gaagcgctgg
     6541 caaagcttaa acctgccttt aaaaaagacg gtactgtaac ggccggaaac gcaagcggta
     6601 taaatgacgc tgctgcggca gttgttgtaa tgtcaaagga aaaggctgat gagcttggaa
     6661 tcaagcctct tgccacaata aaaagctttg gatatgccgg tgtggatcca aagataatgg
     6721 gcattggtcc cgtatacgca acaagaaaag cattggagaa agccaacctc acggtcgatg
     6781 atttggacct tattgaggcc aatgaagctt ttgccgccca gtctcttgct gttgcaaaag
     6841 aacttaagtt taacatggat cgggtaaatg taaacggcgg tgccatagcg attggacatc
     6901 cgataggagc atcgggctgc agaatacttg taactctttt gtatgaaatg cagaaaagaa
     6961 attcccacac cggtctggca actttgtgta taggcggagg aatgggtatt gccatggtgg
     7021 ttgaaagagg tcatcaccac caccaccatt agggtctcgc ggccgccacc gctgagcaat
     7081 aactagcata acccctctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7141 atgagattat caaaaaggat cttcacctag atccttttgg ttcatgtgca gctccatcag
     7201 caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt tgatcgtgct
     7261 atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag
     7321 ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc
     7381 acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg atcgaggccc
     7441 tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg
     7501 acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt gtctctgaca
     7561 cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg
     7621 ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac tcgcctgcaa
     7681 gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc gtgggacacg
     7741 atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat ggggtgccga
     7801 gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc gagaatgacc
     7861 actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc cttcagaagg
     7921 aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt gtggcgacag
     7981 ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag gcgggatgcg
     8041 aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga acgagatgac
     8101 gttggagcta gcttcaacaa ccagagacac ttgggaaata acatatccac agagttatcc
     8161 acattatcca cagcttattt ttaaacagaa tggatatttg cttattgtga attgtggata
     8221 attttgtgga aaaaattttt gtgtaaataa gtcgaaattg gtggtatata tataatatca
     8281 aaacaaaact ttctacatag aaaggatggt ctctagaatg aactttaata aaattgattt
     8341 agacaattgg aagagaaaag agatatttaa tcattatttg aaccaacaaa cgacttttag
     8401 tataaccaca gaaattgata ttagtgtttt ataccgaaac ataaaacaag aaggatataa
     8461 attttaccct gcatttattt tcttagtgac aagggtgata aactcaaata cagcttttag
     8521 aactggttac aatagcgacg gagagttagg ttattgggat aagttagagc cactttatac
     8581 aatttttgat ggtgtatcta aaacattctc tggtatttgg actcctgtaa agaatgactt
     8641 caaagagttt tatgatttat acctttctga tgtagagaaa tataatggtt cggggaaatt
     8701 gtttcccaaa acacctatac ctgaaaatgc tttttctctt tctattattc catggacttc
     8761 atttactggg tttaacttaa atatcaataa taatagtaat taccttctac ccattattac
     8821 agcaggaaaa ttcattaata aaggtaattc aatatattta ccgctatctt tacaggtaca
     8881 tcattctgtt tgtgatggtt atcatgcagg attgtttatg aactctattc aggaattgtc
     8941 agataggcct aatgactggc ttttataata tgagataatg ccgactgtac tttttacagt
     9001 cggttttct
//
