pDamBOB vector (V015705)

LOCUS       V015705                 6035 bp    DNA     circular SYN 05-FEB-2020
DEFINITION  Exported.
ACCESSION   V015705
VERSION     V015705
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Alvarez JM, Schinke A-L., Brooks MD, Pasquino AA, Leonelli L, Varala
            K, Safi A, Krouk G, Krapp A, Coruzzi GM.
  TITLE     Transient genome-wide interactions of the master transcription
            factor NLP7 initiate a rapid nitrogen-response cascade
  JOURNAL   Nat Commun (2020) In press
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Brooks MD, Leonelli L, Alvarez JM, Pasquino AV, Coruzzi GM.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2020) Biology - Center for Genomics and Systems
            Biology, New York University, 12 Waverly Place, New York, NY 10003,
            USA
REFERENCE   3  (bases 1 to 6035)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-JAN-2020) Biology - Center for Genomics and Systems Biology, New
            York University, 12 Waverly Place, New York, NY 10003, USA"
FEATURES             Location/Qualifiers
     source          1..6035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(71..458)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     promoter        755..1100
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1126..1833
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      1845..2096
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2283..2627
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2655..3488
                     /gene="dam"
                     /label="DNA adenine methylase"
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     CDS             3528..3548
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    3558..4391
                     /note="Region: C-terminal glucocorticoid receptor; GR"
     CDS             4401..4427
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     primer_bind     complement(4485..4501)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      4503..4762
                     /label="nos terminator"
                     /note="nos terminator"
                     /regulatory_class="terminator"
     promoter        4990..5094
                     /label="AmpR promoter"
     CDS             5095..5952
                     /label="AmpR"
                     /note="beta-lactamase"