LOCUS       V015705                 6035 bp    DNA     circular SYN 05-FEB-2020
DEFINITION  Exported.
ACCESSION   V015705
VERSION     V015705
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Alvarez JM, Schinke A-L., Brooks MD, Pasquino AA, Leonelli L, Varala
            K, Safi A, Krouk G, Krapp A, Coruzzi GM.
  TITLE     Transient genome-wide interactions of the master transcription
            factor NLP7 initiate a rapid nitrogen-response cascade
  JOURNAL   Nat Commun (2020) In press
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Brooks MD, Leonelli L, Alvarez JM, Pasquino AV, Coruzzi GM.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2020) Biology - Center for Genomics and Systems
            Biology, New York University, 12 Waverly Place, New York, NY 10003,
            USA
REFERENCE   3  (bases 1 to 6035)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-JAN-2020) Biology - Center for Genomics and Systems Biology, New
            York University, 12 Waverly Place, New York, NY 10003, USA"
FEATURES             Location/Qualifiers
     source          1..6035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(71..458)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     promoter        755..1100
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1126..1833
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      1845..2096
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        2283..2627
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2655..3488
                     /gene="dam"
                     /label="DNA adenine methylase"
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     CDS             3528..3548
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    3558..4391
                     /note="Region: C-terminal glucocorticoid receptor; GR"
     CDS             4401..4427
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     primer_bind     complement(4485..4501)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      4503..4762
                     /label="nos terminator"
                     /note="nos terminator"
                     /regulatory_class="terminator"
     promoter        4990..5094
                     /label="AmpR promoter"
     CDS             5095..5952
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 cctttttgat aatctcatga ccaagtccgc tcacaattcc acatgtggaa ttccacatgt
       61 ggaattccca tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt tgagaggctc
      121 taagggcttc tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaaa
      181 gctttaaaag ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt
      241 taagttgctg atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat
      301 gagagcttag tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt
      361 aaacatgaga gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag
      421 tacgtactat caacaggttg aactgctgat gttcagaata cgcaaaccgc ctcaagcttc
      481 tgcaggtccg attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
      541 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
      601 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
      661 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
      721 ttcaaagcaa gtggattgat gtgatggtcc gatgtgagac ttttcaacaa agggtaatat
      781 ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg
      841 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag
      901 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
      961 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
     1021 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
     1081 catttcattt ggagaggaca cgctgacaag ctgactctgc atgctatggt gagcaagggc
     1141 gaggaagata atatggcaat tattaaggaa tttatgaggt ttaaagttca tatggagggg
     1201 tcagtcaatg gtcatgagtt cgagattgaa ggtgaaggtg agggacgtcc atacgaaggt
     1261 actcagaccg ctaagctcaa agttacgaag ggaggaccgc tcccatttgc atgggatatc
     1321 ttgagtcctc aattcatgta cggtagtaaa gcgtacgtca agcatccagc tgacattcct
     1381 gattacctca aattgagctt cccggaagga tttaaatggg aaagagtcat gaacttcgaa
     1441 gatggtggag ttgtcacggt gacccaagat agctctcttc aagatggtga atttatatat
     1501 aaggtcaaac tcagaggtac aaattttccg tctgatggtc cagttatgca aaagaagact
     1561 atgggatggg aagctagttc agaaagaatg tatcctgaag acggagctct caaaggtgag
     1621 attaagcaaa ggctaaagtt aaaagatgga gggcattacg atgctgaggt aaaaacaact
     1681 tacaaggcta agaagccggt gcaactgcct ggtgcttaca atgttaatat caaattagat
     1741 attactagtc ataacgaaga ctacactatt gttgagcaat acgagagggc agagggaagg
     1801 cactctactg gaggcatgga cgagctgtac aagtaaggta ccaattcctt caaacatttg
     1861 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     1921 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     1981 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     2041 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac taaatcgggg
     2101 atagaacccc gcaattggtt cgaggaacta acagaactcg ccgtgaagac tggcgaacag
     2161 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
     2221 cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct
     2281 attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct
     2341 atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat
     2401 tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga
     2461 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
     2521 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     2581 caagaccctt cctctatata aggaagttca tttcatttgg agaggacgtc gaccctgcag
     2641 gcggccgcac tagtatgaag aagaacaggg cattcttgaa gtgggctgga ggaaaatacc
     2701 ctctactcga tgatatcaaa cgtcatcttc caaaaggaga gtgtcttgtg gaacctttcg
     2761 taggagcagg tagcgttttt ctgaacacgg atttctcgcg ttacatcctt gcagatatca
     2821 attcagattt gatttcgttg tacaacattg tcaaaatgcg tacagacgaa tatgtacaag
     2881 cagctaggga gctgttcgtg cctgagacta actgtgcaga ggtttactat cagttccgtg
     2941 aggaatttaa caagtctcaa gaccctttta ggcgtgctgt cctctttttg tatttgaaca
     3001 gatacggata caacggactc tgcaggtaca atctaagagg agaatttaat gtaccgtttg
     3061 gaaggtacaa gaaaccatat tttcctgaag ctgaattata tcatttcgct gagaaggctc
     3121 aaaatgcatt tttttattgt gagtcgtatg ctgattctat ggctagggct gacgacgctt
     3181 ctgttgttta ttgtgaccca ccttacgctc cattatcggc taccgctaat tttaccgcat
     3241 accacactaa tagcttcact ctggagcaac aagctcatct cgctgagata gctgagggat
     3301 tggttgaaag gcacatccct gttcttatat cgaaccacga tactatgttg accagagaat
     3361 ggtatcaaag agcaaaactc cacgtggtga aggttcgtag aagtatttct agtaatggtg
     3421 gtacacgtaa aaaggtcgat gagttgctag cattgtacaa acctggggtc gtgagtccag
     3481 caaaaaaagc tggagagcag aagctcattt ctgaggagga tggtgcacct aagaagaaga
     3541 gaaaggtggg aggtggactg attcagcaag ccactgcagg agtctcacaa gacacttcgg
     3601 aaaatcctaa caaaacaata gttcctgcag cattaccaca gctcacccct accttggtgt
     3661 cactgctgga ggtgattgaa cccgaggtgt tgtatgcagg atatgatagc tctgttccag
     3721 attcagcatg gagaattatg accacactca acatgttagg tgggcgtcaa gtgattgcag
     3781 cagtgaaatg ggcaaaggcg atactaggct tgagaaactt acacctcgat gaccaaatga
     3841 ccctgctaca gtactcatgg atgtttctca tggcatttgc cttgggttgg aggtcataca
     3901 gacaatcaag cggaaacctg ctctgctttg ctcctgacct gattattaat gagcagagaa
     3961 tgtctctacc ctgcatgtat gaccaatgta aacacatgct gtttgtctcc tctgaattac
     4021 aaagattgca ggtatcctat gaagagtatc tctgtatgaa aaccttactg cttctctcct
     4081 cagttcctaa ggaaggtctg aagagccaag agttatttga tgagattcga atgacttata
     4141 tcaaagagct aggaaaagcc atcgtcaaaa gggaagggaa ctccagtcag aactggcaac
     4201 ggttttacca actgacaaag ctcctggact ccatgcatga ggtggttgag aatctcctta
     4261 cctactgctt ccagacattt ttggataaga ccatgagtat tgagttccca gagatgttag
     4321 ctgaaatcat cactaatcag ataccaaaat attcaaatgg aaatatcaaa aagctcctgt
     4381 ttcatcaaaa actcgagacc tatccttacg acgtgccaga ttatgcttga ggtctccgga
     4441 attcttaagg acaaccagtt ggcgggacta gaaacatgac acttactggc cgtcgtttta
     4501 caaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     4561 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     4621 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     4681 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     4741 ctagagcggg gaccgattcc cgcttaacca gctccaccag cactcaaccc tatctcggtc
     4801 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4861 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc
     4921 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg
     4981 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5041 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagc
     5101 attcaacact ttcgtgtggc actgataccg tttttcgctg ccttttgcct gccagttttc
     5161 gcacacccgg aaaccctggt gaaagtaaag gacgcggagg accagctggg tgcccgtgtg
     5221 ggttatattg aactggattt aaactccggc aaaattctgg agagcttccg cccggaagaa
     5281 cgctttccga tgatgtctac tttcaaagtt ctgctgtgcg gcgcagtact gagccgcatt
     5341 gatgccggtc aggagcaact gggccgtcgt attcattatt ctcagaacga cctggtagaa
     5401 tattctccgg taactgagaa acacctgacc gatggtatga ccgtgcgtga gctgtgtagc
     5461 gcagcgataa ccatgtctga taacaccgcg gccaacctgc tgctgaccac cattggtggt
     5521 ccgaaagaac tgaccgcctt tctgcataac atgggcgacc atgtgacccg tctggatagg
     5581 tgggagccgg aactgaacga agcaattccg aacgatgagc gtgataccac catgccggtt
     5641 gctatggcga ccactctgcg taaactgctg accggcgagc tgctgaccct ggccagccgc
     5701 caacagctga tagactggat ggaggcagac aaagttgctg gtccgctgct gcgttctgcc
     5761 ctgccagctg gctggtttat cgctgataaa agcggcgccg gtgaacgtgg ctcccgcggt
     5821 atcatcgcgg ccctgggtcc ggatggcaag ccgtcacgta ttgtggttat ctacaccacc
     5881 ggtagccagg ctaccatgga tgagcgtaac cgccagattg cagaaatcgg tgcatccctg
     5941 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6001 cttcattttt aatttaaaag catctaggtg aagtt
//
