pP10 vector (V015704)

LOCUS       62056_18340        3166 bp RNA     circular SYN 15-JAN-2020
DEFINITION  Cloning vector pP10, complete sequence.
ACCESSION   MN702625
VERSION     .
KEYWORDS    .
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Liu X, Yang X, Mehboob A, Hu X, Yi Y, Li Y, Zhang Z.
  TITLE     A construction strategy for a baculovirus-silkworm multigene 
            expression system and its application for coexpression of type I and
            type II interferons
  JOURNAL   Microbiologyopen, e979 (2020) In press
  PUBMED    31854114
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2019) Biotechnology Research Institute, Chinese 
            Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, 
            Beijing 100081, China
REFERENCE   3  (bases 1 to 3166)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiologyopen, e979 (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-NOV-2019) Biotechnology Research Institute, Chinese Academy of 
            Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 
            100081, China"
FEATURES             Location/Qualifiers
     source          1..3166
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    451..682
                     /label=BmNPV p10 gene recombination sequence
                     /note="BmNPV p10 gene recombination sequence"
     misc_feature    683..762
                     /label=MCS
                     /note="MCS"
     misc_feature    763..880
                     /label=BmNPV p10 gene recombination sequence
                     /note="BmNPV p10 gene recombination sequence"
     primer_bind     complement(945..961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(969..985)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(993..1023)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1038..1059)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1347..1935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2109..2966)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2967..3071)
                     /label=AmpR promoter