pP10 vector (V015704)

MSDS

Basic Vector Information

Vector Name:: pP10

Antibiotic Resistance:: Ampicillin

Length:: 3166 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Liu X, Yang X, Mehboob A, Hu X

pP10 vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pP10 vector Sequence

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Download GeneBank File(.gb)

LOCUS       62056_18340        3166 bp RNA     circular SYN 15-JAN-2020
DEFINITION  Cloning vector pP10, complete sequence.
ACCESSION   MN702625
VERSION     .
KEYWORDS    .
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Liu X, Yang X, Mehboob A, Hu X, Yi Y, Li Y, Zhang Z.
  TITLE     A construction strategy for a baculovirus-silkworm multigene 
            expression system and its application for coexpression of type I and
            type II interferons
  JOURNAL   Microbiologyopen, e979 (2020) In press
  PUBMED    31854114
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2019) Biotechnology Research Institute, Chinese 
            Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, 
            Beijing 100081, China
REFERENCE   3  (bases 1 to 3166)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiologyopen, e979 (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-NOV-2019) Biotechnology Research Institute, Chinese Academy of 
            Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 
            100081, China"
FEATURES             Location/Qualifiers
     source          1..3166
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    451..682
                     /label=BmNPV p10 gene recombination sequence
                     /note="BmNPV p10 gene recombination sequence"
     misc_feature    683..762
                     /label=MCS
                     /note="MCS"
     misc_feature    763..880
                     /label=BmNPV p10 gene recombination sequence
                     /note="BmNPV p10 gene recombination sequence"
     primer_bind     complement(945..961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(969..985)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(993..1023)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1038..1059)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1347..1935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2109..2966)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2967..3071)
                     /label=AmpR promoter

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