LOCUS       62056_18340        3166 bp RNA     circular SYN 15-JAN-2020
DEFINITION  Cloning vector pP10, complete sequence.
ACCESSION   MN702625
VERSION     .
KEYWORDS    .
SOURCE      
  ORGANISM  
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Liu X, Yang X, Mehboob A, Hu X, Yi Y, Li Y, Zhang Z.
  TITLE     A construction strategy for a baculovirus-silkworm multigene 
            expression system and its application for coexpression of type I and
            type II interferons
  JOURNAL   Microbiologyopen, e979 (2020) In press
  PUBMED    31854114
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Liu X.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2019) Biotechnology Research Institute, Chinese 
            Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, 
            Beijing 100081, China
REFERENCE   3  (bases 1 to 3166)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiologyopen, e979 (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-NOV-2019) Biotechnology Research Institute, Chinese Academy of 
            Agricultural Sciences, No. 12 Zhongguancun South Street, Beijing 
            100081, China"
FEATURES             Location/Qualifiers
     source          1..3166
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    451..682
                     /label=BmNPV p10 gene recombination sequence
                     /note="BmNPV p10 gene recombination sequence"
     misc_feature    683..762
                     /label=MCS
                     /note="MCS"
     misc_feature    763..880
                     /label=BmNPV p10 gene recombination sequence
                     /note="BmNPV p10 gene recombination sequence"
     primer_bind     complement(945..961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(969..985)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(993..1023)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1038..1059)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1347..1935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2109..2966)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2967..3071)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa agaagcatga cggcaagtgg
      421 acgattgata tcccaatgca ttggaagctt tggagtcttg tgtgctattt tacagagatt
      481 cagaaatacg catcacttac aacagggggg actatgaaat tatgcatttg aggatgccgg
      541 gacctttaat tcaacccaac acaatatatt acagctaaat aagaattatt attaaattat
      601 ttgtatatta attaaaatct tatactgtaa attacatttt atttactatc atttcaaagc
      661 ctaacgtttt gacacgaatt ttggatccct gcaggcctcg agttcgaatc tagaagatct
      721 ggtaccgagc tcgaattccc gggcggccgc ttaattaatt gagacggttt gcccgctcaa
      781 ttgactgatt ttaacactaa aatctcagaa attcaatcca tattgaccgg tgacactgct
      841 ccggaccctc cagaatccta gagcctaacc tgaaaagcca gtcgacacta gtatcgataa
      901 tctccagagg atcgccggga accgaggacg agttcgtaat catggtcata gctgtttcct
      961 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     1021 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     1081 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1141 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1201 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1261 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1321 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1381 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1441 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1501 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1561 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1621 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1681 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1741 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1801 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1861 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1921 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1981 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2041 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2101 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2161 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2221 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2281 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2341 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2401 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2461 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2521 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2581 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2641 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2701 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2761 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2821 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2881 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2941 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3001 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3061 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     3121 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
