L1611 vector (V015701)

MSDS

Basic Vector Information

Vector Name:: L1611

Antibiotic Resistance:: Kanamycin

Length:: 2538 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Doll NM, Gilles LM, Gerentes MF, Richard C

L1611 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

L1611 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_1375        2538 bp DNA     circular SYN 10-FEB-2019
DEFINITION  Cloning vector L1611, complete sequence.
ACCESSION   MH662440
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Doll NM, Gilles LM, Gerentes MF, Richard C, Just J, Fierlej Y, 
            Borrelli VM, Gendrot G, Ingram GC, Rogowsky PM, Widiez T.
  TITLE     Single and multiple gene knockouts by CRISPR-Cas9 in maize
  JOURNAL   Plant Cell Rep. (2019) In press
  PUBMED    30684023
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Doll NM, Gilles LM, Gerentes M-F., Richard C, Just J, Barrelli VMG.,
            Gendrot G, Ingram GC, Rogowsky PM, Widiez T.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2018) ENS de Lyon, INRA, 46 allee d'Italie, Lyon 
            69364, France
REFERENCE   3  (bases 1 to 2538)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JUL-2018) ENS de Lyon, INRA, 46 allee d'Italie, Lyon 69364, 
            France"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      205..564
                     /label=pTaU6
                     /note="pTaU6"
                     /regulatory_class="promoter"
     misc_RNA        592..667
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      668..676
                     /label=terminator
                     /note="terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(839..855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      903..934
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        935..1037
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1038..1850
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      1877..1904
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1916..2503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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