LOCUS       62056_1375        2538 bp DNA     circular SYN 10-FEB-2019
DEFINITION  Cloning vector L1611, complete sequence.
ACCESSION   MH662440
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Doll NM, Gilles LM, Gerentes MF, Richard C, Just J, Fierlej Y, 
            Borrelli VM, Gendrot G, Ingram GC, Rogowsky PM, Widiez T.
  TITLE     Single and multiple gene knockouts by CRISPR-Cas9 in maize
  JOURNAL   Plant Cell Rep. (2019) In press
  PUBMED    30684023
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Doll NM, Gilles LM, Gerentes M-F., Richard C, Just J, Barrelli VMG.,
            Gendrot G, Ingram GC, Rogowsky PM, Widiez T.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2018) ENS de Lyon, INRA, 46 allee d'Italie, Lyon 
            69364, France
REFERENCE   3  (bases 1 to 2538)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JUL-2018) ENS de Lyon, INRA, 46 allee d'Italie, Lyon 69364, 
            France"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      205..564
                     /label=pTaU6
                     /note="pTaU6"
                     /regulatory_class="promoter"
     misc_RNA        592..667
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      668..676
                     /label=terminator
                     /note="terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(839..855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      903..934
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        935..1037
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1038..1850
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      1877..1904
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1916..2503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc cccgggcgga tatcgaccaa gcccgttatt ctgacagttc tggtgctcaa
      241 cacatttata tttatcaagg agcacattgt tactcactgc taggagggaa tcgaactagg
      301 aatattgatc agaggaacta cgagagagct gaagataact gccctctagc tctcactgat
      361 ctgggtcgca tagtgagatg cagcccacgt gagttcagca acggtctagc gctgggcttt
      421 taggcccgca tgatcgggct tttgtcgggt ggtcgacgtg ttcacgattg gggagagcaa
      481 cgcagcagtt cctcttagtt tagtcccacc tcgcctgtcc agcagagttc tgaccggttt
      541 ataaactcgc ttgctgcatc agacttggga agagcagata tcagctcttc agttttagag
      601 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      661 tcggtgcttt ttttttaact ataacggtcc taaggtagcg aaggatccgc cccgggggat
      721 cgacgagagc agcgcgactg gatcactact ggaccgcgag ctgtgctgcg acccgtgatc
      781 ttacggcatt atacgtatga tcggtccacg atcagctaga ttatctagtc agcttgatgt
      841 catagctgtt tcctgaggct caatactgac catttaaatc atacctgacc tccatagcag
      901 aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact
      961 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     1021 gagctaagga agctaaaatg agccatattc aacgggaaac gtcttgcttg aagccgcgat
     1081 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     1141 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
     1201 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcagg ctaaactggc
     1261 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     1321 ggttactcac cactgcgatc ccagggaaaa cagcattcca ggtattagaa gaatatcctg
     1381 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     1441 ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg tctcgctcag gcgcaatcac
     1501 gaatgaataa cggtttggtt ggtgcgagtg attttgatga cgagcgtaat ggctggcctg
     1561 ttgaacaagt ctggaaagaa atgcataaac tcttgccatt ctcaccggat tcagtcgtca
     1621 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     1681 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     1741 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     1801 atcctgatat gaataaattg cagtttcact tgatgctcga tgagtttttc taatgaggac
     1861 ctaaatgtaa tcacctggct caccttcggg tgggcctttc tgcgttgctg gcgtttttcc
     1921 ataggctccg cccccctgac gagcatcaca aaaatcgatg ctcaagtcag aggtggcgaa
     1981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2341 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttacctcgg
     2401 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2461 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatttt
     2521 ctaccgaaga aaggccca
//
