Hym-176C_EGFP vector (V015667)

LOCUS       62056_1281        4856 bp DNA     circular SYN 23-JAN-2019
DEFINITION  Expression vector Hym-176C_EGFP DNA, complete sequence.
ACCESSION   LC426374
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Noro Y, Yum S, Nishimiya-Fujisawa C, Busse C, Shimizu H, Mineta K, 
            Zhang X, Holstein TW, David CN, Gojobori T, Fujisawa T.
  TITLE     Regionalized nervous system in Hydra and the mechanism of its 
            development
  JOURNAL   Gene Expr. Patterns (2019) In press
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Noro Y, Fujisawa T, Shimizu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2018) Contact:Yukihiko Noro Waseda University, 
            Center for Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho, 
            Shinjuku-ku, Tokyo 162-8480, Japan
REFERENCE   3  (bases 1 to 4856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene Expr. 
            Patterns (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2018) Contact:Yukihiko Noro Waseda University, Center for 
            Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho, 
            Shinjuku-ku, Tokyo 162-8480, Japan"
FEATURES             Location/Qualifiers
     source          1..4856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        362..466
                     /label=AmpR promoter
     CDS             467..1324
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1498..2086
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2374..2395
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2410..2440
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2448..2464
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2472..2488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3552..4265
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"