LOCUS       62056_1281        4856 bp DNA     circular SYN 23-JAN-2019
DEFINITION  Expression vector Hym-176C_EGFP DNA, complete sequence.
ACCESSION   LC426374
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Noro Y, Yum S, Nishimiya-Fujisawa C, Busse C, Shimizu H, Mineta K, 
            Zhang X, Holstein TW, David CN, Gojobori T, Fujisawa T.
  TITLE     Regionalized nervous system in Hydra and the mechanism of its 
            development
  JOURNAL   Gene Expr. Patterns (2019) In press
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Noro Y, Fujisawa T, Shimizu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2018) Contact:Yukihiko Noro Waseda University, 
            Center for Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho, 
            Shinjuku-ku, Tokyo 162-8480, Japan
REFERENCE   3  (bases 1 to 4856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene Expr. 
            Patterns (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2018) Contact:Yukihiko Noro Waseda University, Center for 
            Advanced Biomedical Sciences (TWIns); 2-2 Wakamatsu-cho, 
            Shinjuku-ku, Tokyo 162-8480, Japan"
FEATURES             Location/Qualifiers
     source          1..4856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        362..466
                     /label=AmpR promoter
     CDS             467..1324
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1498..2086
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2374..2395
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2410..2440
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2448..2464
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2472..2488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3552..4265
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
       61 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
      121 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
      181 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
      241 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
      301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      481 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      541 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      601 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      661 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      721 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      781 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      841 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
      901 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      961 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1021 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1081 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1141 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1201 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1261 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1321 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1381 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1621 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1741 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1801 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1861 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     1921 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1981 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2041 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2101 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2161 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2221 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2281 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2341 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2401 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2461 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcatgc
     2521 tcgagcggcc gccagtgtga tggatatctg cagaattcgg ctttcgtacg agaatcgctg
     2581 tcctctccaa cgagccaaga agcttaaaag ttttcaaaaa aaaaaagtta gaaaaatgta
     2641 aaataattta gaaaaaaaaa gttatatctt ttcttattta gcaagtttgt tattacttcc
     2701 aaaataatac aaatctattt tggtaaaatg tttgataaaa taatgtaaaa aaggaattaa
     2761 atttttttaa ctcttgtttt tcattaacta gaatttcatt gtggtgctta gcccgcccta
     2821 caacatattt tcaatactaa aaggatatca ggtgaaactt taaatgtgtt aacgaatgta
     2881 aatttagatt tgtaaaaaac ttattgtttt attgacctca cagttgggat agcggggtga
     2941 tgggacaata tttcggtttt tcactatcgt tatatttaac tatttcaatt tgtttcttta
     3001 gacaacacga aatattgggt taaattgaat ccaataagga aacagcaact aaaattaaac
     3061 gatgttcact cacttaaaca ttactactat gacatctaat tgaaattgtg cagaaaaaac
     3121 agtctgaaca aagtaacatc tctgaatggt agtcaaaagt atttttggtc ataaatatac
     3181 gaaaactatt tatatcttct taaacgtttt ttccatataa ataattaaca tggaagttat
     3241 actattaaaa ttagaaagga acttcctaat cttgtagtca cggtttgttt ttttatttcc
     3301 ttgtaagttc cttttttaat tttttaattt tgcgtatttg tagatgttta ctttatatac
     3361 aatgagaaga aattggtttt aaaaagagtt ccttcctgaa ataataaatt ctatttcact
     3421 ttaagaggaa gaaatataca caatagaacc tttatcaaaa aattttctta ttcattgaaa
     3481 ttatgacttt aaatttctat aaacacatac tgaaacaatt agaaaactgt tcgatctagc
     3541 ccggggaaaa aatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     3601 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     3661 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     3721 ggccaacact tgtcactact ttctgttatg gtgttcaatg cttttcaaga tacccagatc
     3781 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     3841 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     3901 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     3961 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     4021 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     4081 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     4141 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     4201 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     4261 acaaatagca ttcgtagaat tcaaacacaa atgcactttt aattttattt atagtttgat
     4321 aaccgtacac tatatttatt tacgctataa aatgtaatga aaattaaaaa aaataatgct
     4381 atttgtatga tttaatgagt tttgatttaa taaaatcaaa tttgttttat atgttttatt
     4441 aatgttattt tattaataat actttaaaat tatcatctag tgaatccact cacatatctt
     4501 atattagtgg tttcccgata aagacttgag gtgaacaaga gaccgttgaa attaaataaa
     4561 aactttttgc tttttgacac tttttctgaa actgtcttta aaaaaatctt tgaaaaaaaa
     4621 atgaaagaat ttcaaacttt atacgctcat accttttcag caccaaaatt gatttaaaat
     4681 ttttttttga aatttattga tgaactttta tttaatttaa gaaaatcaaa ctttgttttt
     4741 tcaaagctga ttacctaaat cggtaggaac atttttgata gcagatttct ctggctcatt
     4801 caagattgtt caaataagcg atttccaaaa agtttagact aacagtattt gacctt
//
