pLCBS1 vector (V015619)

LOCUS       62056_13745        9993 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLCBS1, complete sequence.
ACCESSION   MK176925
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..9993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             220..2004
                     /codon_start=1
                     /transl_table=11
                     /product="phiBT1 integrase"
                     /label=phiBT1 integrase
                     /protein_id="QCO69788.1"
                     /translation="MSPFIAPDVPEHLLDTVRVFLYARQSKGRSDGSDVSTEAQLAAGR
                     ALVASRNAQGGARWVVAGEFVDVGRSGWDPNVTRADFERMMGEVRAGEGDVVVVNELSR
                     LTRKGAHDALEIDNELKKHGVRFMSVLEPFLDTSTPIGVAIFALIAALAKQDSDLKAER
                     LKGAKDEIAALGGVHSSSAPFGMRAVRKKVDNLVISVLEPDEDNPDHVELVERMAKMSF
                     EGVSDNAIATTFEKEKIPSPGMAERRATEKRLASIKARRLNGAEKPIMWRAQTVRWILN
                     HPAIGGFAFERVKHGKAHINVIRRDPGGKPLTPHTGILSGSKWLELQEKRSGKNLSDRK
                     PGAEVEPTLLSGWRFLGCRICGGSMGQSQGGRKRNGDLAEGNYMCANPKGHGGLSVKRS
                     ELDEFVASKVWARLRTADMEDEHDQAWIAAAAERFALQHDLAGVADERREQQAHLDNVR
                     RSIKDLQADRKAGLYVGREELETWRSTVLQYRSYEAECTTRLAELDEKMNGSTRVPSEW
                     FSGEDPTAEGGIWASWDVYERREFLSFFLDSVMVDRGRHPETKKYIPLKDRVTLKWAEL
                     LKEEDEASEATERELAAL"
     CDS             complement(2306..3949)
                     /codon_start=1
                     /transl_table=11
                     /product="SV1 integrase"
                     /label=SV1 integrase
                     /protein_id="QCO69789.1"
                     /translation="MKRDLPSTFRGSRTPGEPWLGYIRVSTWREEKISPELQQSAIESW
                     AARTGRRIVDWIVDLDATGRNFKRKIMGGIQRVEGREAVGIAVWKFSRFGRNDLGIAIN
                     LARLEQAGGDLASATEEVDARTAVGRFNRAILFDLAVFESDRAGEQWKETHAHRRALKL
                     PATGRQRFGYVWHPRRVPDLTAPGGFRLQEERYERHPEFAPVAAELYERKLAGQGFSQL
                     AYWLNDELLIPTTRGNRWGTNTVQRYLDSGFAAGLLRVHDPECRCKLGQDHFSACKENR
                     WLWLPGAQPALIVPEQWKEYGAHREQTRKTPPRARRASYPTSGIMRHGHCRGTAVARSG
                     RDGKGGFVPGHVFVCFNRRNKGKSACEPGLYVRRDEVEAEVLKWLADTVADDIDNAPAL
                     PAQRTAPGTAPDPRARLVEERTRTEAELAKIEGALDRLVTDYALDPDKYPADTFGRVRD
                     QLLGKKGDIIKHLKSLSEVEVAPTREEFRPLIVGLLQEWDILHTTEKNAILRRLLRRLV
                     IHNRKSDQGAQWSVVRSFEFHPVWEPDPWS"
     promoter        complement(4785..4815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4830..4851)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5139..5727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5980..6780
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(7217..7585)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(7618..7727)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(7965..7976)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    8047..8146
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             8156..9970
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"