LOCUS       62056_13745        9993 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLCBS1, complete sequence.
ACCESSION   MK176925
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..9993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             220..2004
                     /codon_start=1
                     /transl_table=11
                     /product="phiBT1 integrase"
                     /label=phiBT1 integrase
                     /protein_id="QCO69788.1"
                     /translation="MSPFIAPDVPEHLLDTVRVFLYARQSKGRSDGSDVSTEAQLAAGR
                     ALVASRNAQGGARWVVAGEFVDVGRSGWDPNVTRADFERMMGEVRAGEGDVVVVNELSR
                     LTRKGAHDALEIDNELKKHGVRFMSVLEPFLDTSTPIGVAIFALIAALAKQDSDLKAER
                     LKGAKDEIAALGGVHSSSAPFGMRAVRKKVDNLVISVLEPDEDNPDHVELVERMAKMSF
                     EGVSDNAIATTFEKEKIPSPGMAERRATEKRLASIKARRLNGAEKPIMWRAQTVRWILN
                     HPAIGGFAFERVKHGKAHINVIRRDPGGKPLTPHTGILSGSKWLELQEKRSGKNLSDRK
                     PGAEVEPTLLSGWRFLGCRICGGSMGQSQGGRKRNGDLAEGNYMCANPKGHGGLSVKRS
                     ELDEFVASKVWARLRTADMEDEHDQAWIAAAAERFALQHDLAGVADERREQQAHLDNVR
                     RSIKDLQADRKAGLYVGREELETWRSTVLQYRSYEAECTTRLAELDEKMNGSTRVPSEW
                     FSGEDPTAEGGIWASWDVYERREFLSFFLDSVMVDRGRHPETKKYIPLKDRVTLKWAEL
                     LKEEDEASEATERELAAL"
     CDS             complement(2306..3949)
                     /codon_start=1
                     /transl_table=11
                     /product="SV1 integrase"
                     /label=SV1 integrase
                     /protein_id="QCO69789.1"
                     /translation="MKRDLPSTFRGSRTPGEPWLGYIRVSTWREEKISPELQQSAIESW
                     AARTGRRIVDWIVDLDATGRNFKRKIMGGIQRVEGREAVGIAVWKFSRFGRNDLGIAIN
                     LARLEQAGGDLASATEEVDARTAVGRFNRAILFDLAVFESDRAGEQWKETHAHRRALKL
                     PATGRQRFGYVWHPRRVPDLTAPGGFRLQEERYERHPEFAPVAAELYERKLAGQGFSQL
                     AYWLNDELLIPTTRGNRWGTNTVQRYLDSGFAAGLLRVHDPECRCKLGQDHFSACKENR
                     WLWLPGAQPALIVPEQWKEYGAHREQTRKTPPRARRASYPTSGIMRHGHCRGTAVARSG
                     RDGKGGFVPGHVFVCFNRRNKGKSACEPGLYVRRDEVEAEVLKWLADTVADDIDNAPAL
                     PAQRTAPGTAPDPRARLVEERTRTEAELAKIEGALDRLVTDYALDPDKYPADTFGRVRD
                     QLLGKKGDIIKHLKSLSEVEVAPTREEFRPLIVGLLQEWDILHTTEKNAILRRLLRRLV
                     IHNRKSDQGAQWSVVRSFEFHPVWEPDPWS"
     promoter        complement(4785..4815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4830..4851)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5139..5727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             5980..6780
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(7217..7585)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(7618..7727)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(7965..7976)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    8047..8146
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     CDS             8156..9970
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
ORIGIN
        1 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
       61 gctctcgcag ggcgaagttt aaactggcgc cggacggggc ttcagacgtt tcgggtgctg
      121 ggttgttgtc tctggacagt gatccatggg aaactactca gcaccaccaa tgttcccaaa
      181 agaaagcgca ggtcagcgcc catgagccaa gatctaggca tgtcgccctt catcgctccc
      241 gacgtccctg agcaccttct agacactgtt cgcgtcttcc tgtacgcgcg tcagtctaag
      301 ggccggtccg acggctcaga cgtgtcgacc gaagcacagc tagcggccgg tcgtgcgttg
      361 gtcgcgtctc gcaacgccca ggggggtgcg cgctgggtcg tggcaggtga gttcgtggac
      421 gtcgggcgct ccggctggga cccgaacgtg acccgtgccg acttcgagcg catgatgggc
      481 gaagtccgcg ccggcgaagg tgacgttgtc gttgtgaatg agctttcccg gctcactcgc
      541 aagggcgccc atgacgcgct cgaaatcgac aacgaattga agaagcacgg cgtgcgcttc
      601 atgtcggttc ttgagccgtt ccttgacacg tctaccccta tcggcgtcgc cattttcgcg
      661 ctgatcgctg cccttgcgaa acaggacagt gacctgaagg cggagcgcct gaagggtgcg
      721 aaagacgaga ttgccgcgct gggtggcgtt cactcgtctt ccgccccgtt cggaatgcgc
      781 gccgtgcgca agaaggtcga taatctcgtg atctccgttc ttgagccgga cgaagacaac
      841 ccggatcacg tcgagctagt tgagcgcatg gcgaaaatgt cgttcgaagg cgtgtccgac
      901 aacgccattg caacgacctt cgagaaggaa aagatcccgt cgcccggaat ggctgagaga
      961 cgcgccacgg aaaagcgtct tgcgtccatc aaggcacgtc gcctgaacgg cgctgaaaag
     1021 ccgatcatgt ggcgcgctca aacggtccga tggattctca accatcccgc aatcggcggt
     1081 ttcgcattcg agcgtgtgaa gcacggtaag gcgcacatca acgtcatacg gcgcgacccc
     1141 ggcggcaagc cgctaacgcc ccacacgggc attctcagcg gctcgaagtg gcttgagctt
     1201 caagagaagc gttccgggaa gaatctcagc gaccggaagc ctggggccga agtcgaaccg
     1261 acgcttctga gcgggtggcg tttcctgggg tgccgaatct gcggcggctc aatgggtcag
     1321 tcccagggtg gccgtaagcg caacggcgac cttgccgaag gcaattacat gtgcgccaac
     1381 ccgaaggggc acggcggctt gtcggtcaag cgcagcgaac tggacgaatt cgttgcttcg
     1441 aaggtgtggg cacggctccg cacagccgac atggaagatg aacacgatca ggcatggatt
     1501 gccgccgctg cggagcgctt cgcccttcag cacgacctag cgggggtggc cgatgagcgg
     1561 cgcgaacaac aggcgcacct agacaacgtg cggcgctcca tcaaggacct tcaggcggac
     1621 cgtaaggccg gtctgtacgt cgggcgtgaa gagctggaaa cgtggcgctc aacggtgctg
     1681 caataccggt cctacgaagc ggagtgcacg acccgactcg ctgagcttga cgagaagatg
     1741 aacggcagca cccgcgttcc gtctgagtgg ttcagcggcg aagacccgac ggccgaaggg
     1801 ggcatctggg caagctggga cgtgtacgag cgtcgggagt tcctgagctt cttccttgac
     1861 tccgtcatgg tcgaccgggg gcgccaccct gagacgaaga aatacatccc cctgaaggac
     1921 cgtgtgacgc tcaagtgggc ggagctgctg aaggaggaag acgaagcgag cgaagccact
     1981 gagcgggagc ttgcggcgct gtagcgcaca gcgggagggg tcgagccggc ggacggttcg
     2041 gccccttttt tggccttgaa atcgttagtt aggctaatgc atagacgcgg tgggccgcac
     2101 atggaacggc cccggtacct gaggtaggta ccggggccgt tcttcgcgct ccgatgtggt
     2161 cctttagatc cactgacgtg ggtcagtgtc tctaaaggac tcgcgagcat cgttccccct
     2221 ctccctgcac tgaggtgcgg ttttcagagg gtggcagcag gcggagaaac aatcaagcgc
     2281 gcctgcatgg tttgcgctcc agcggtcaag accaggggtc gggctcccac acgggatgaa
     2341 actcgaagct gcgcacgaca ctccactgtg cgccctgatc gctcttacgg ttgtggatga
     2401 ccagacggcg cagcaggcgg cgcaggatgg cgttcttctc ggtggtgtgc aggatgtccc
     2461 actcctgcaa gagcccgacg atcagggggc ggaactcctc ccgcgtgggc gcgacttcaa
     2521 cctcgctgag cgacttcaag tgcttgatga tgtcgccctt cttaccgagt agctggtcgc
     2581 gtacgcgccc gaacgtgtcc gccgggtact tgtccgggtc cagcgcgtaa tccgtgacga
     2641 gacggtcgag tgcaccctcg atcttggcca actcggcttc cgtgcgggtg cgctcctcga
     2701 ccaggcgggc ccgcgggtcc ggcgcggtgc ccggcgcggt gcgctgagcg ggaagggccg
     2761 gcgcgttgtc gatgtcgtcg gcgaccgtgt cggcgagcca cttcaacacc tcggcctcga
     2821 cttcgtcgcg gcgcacgtag aggccgggct cacaggccga cttccccttg ttcctgcggt
     2881 tgaagcacac gaacacgtgg cccggaacga atccgccctt cccgtcgcgg ccggatcgcg
     2941 caacggccgt cccgcggcag tgtccgtgcc gcatgatgcc gctggtcggg tatgaggccc
     3001 ggcgggcgcg cgggggcgtc ttgcgcgtct gctctctgtg cgccccgtac tccttccact
     3061 gctcgggaac gatgagcgcc ggctgtgccc cggggagcca gagccaccgg ttttctttgc
     3121 acgcagagaa gtggtcctgt cccagcttgc accggcactc cgggtcgtgg acgcgtagca
     3181 ggccggcggc gaatccggag tcgaggtagc gctgaacggt gttggtgccc cagcggttgc
     3241 cccgtgtggt ggggatgagt agttcgtcgt tcagccagta ggcgagctgg gagaacccct
     3301 gtccggcgag ctttcgctcg tagagttcgg ccgccacggg ggcgaactct gggtgccgtt
     3361 cgtagcgctc ctcttgcagg cggaacccgc ccggcgcggt gaggtcgggt acgcgcctcg
     3421 ggtgccacac gtagccgaac cgctgacgcc cggtggcggg gagtttcagg gcccgacggt
     3481 gggcgtgcgt ctccttccac tgttcgccgg cccgatccga ctcgaatacg gcgaggtcga
     3541 acagaatcgc gcggttgaag cgtccgacgg ccgtgcgggc gtcgacttct tccgtggcgg
     3601 acgcgaggtc tccgccggcc tgttcgaggc gggcgaggtt gatagcgatg cccaggtcgt
     3661 tccggccgaa gcggctgaac ttccatacgg cgattccgac ggcctcgcgg ccctcgacgc
     3721 gctggatgcc gcccatgatc ttccgcttga agttgcggcc cgtagcgtcg aggtcaacga
     3781 tccagtcgac gatccgacgt cccgttcggg cggcccatga ctcgatcgcg gattgctgta
     3841 gctccgggct gatcttctcc tcgcgccatg tgctgaccct gatgtagccg agccacggct
     3901 cgcccggcgt gcgggagccg cggaacgtgc ttggtaggtc tcgtttcata tgtggatcct
     3961 accaaccggc acgattgtgc ccacaacagc atcgcggtgc cacgtgtgga ccgcgtcggt
     4021 cagatcctcc ccgcacctct cgccagccgt caagatcgac cgcgtgcacc tgcgatcgcc
     4081 gatcaaccgc gactagcatc gggcgcaagc cgccactcga acggacactc gcatgagatg
     4141 cggccgccgc aattgattta aattgaagcc gccgaagccc cgaagcttcc ccttgaccaa
     4201 tactagtgaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     4261 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt gatcggcact ttgcatcggc
     4321 cgcgctcccg attccggaag tgcttgacat tggggaattt atgcggtgtg aaataccgca
     4381 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     4441 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     4501 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     4561 gacggccagt gccaagcttg ggctgcaggt cgactctaga ggatccttaa ttaaggcata
     4621 tggacactcc ttacttagac tgtcgtattc tcctggccac gactttacaa caccgcacag
     4681 catgttgtca aagcagagac ggttcgaatg tgaacagata tcgaattcgt aatcatgtca
     4741 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4801 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4861 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4921 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4981 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5041 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5101 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5161 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5221 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5281 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5341 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5401 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5461 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5521 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5581 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5641 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5701 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tctactagta gctccatcag
     5761 caaaagggga tgataagttt atcaccaatt taaatctacg gggtctgacg ctcagtggaa
     5821 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5881 ccttttggtt catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac
     5941 tatttgcaac agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc
     6001 aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct caaccttggg
     6061 gttacccccg gcggtgtgct gctggtccac agctccttcc gtagcgtccg gcccctcgaa
     6121 gatgggccac ttggactgat cgaggccctg cgtgctgcgc tgggtccggg agggacgctc
     6181 gtcatgccct cgtggtcagg tctggacgac gagccgttcg atcctgccac gtcgcccgtt
     6241 acaccggacc ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt aaagcgcagc
     6301 gcccatccat ttgcctttgc ggcagcgggg ccacaggcag agcagatcat ctctgatcca
     6361 ttgcccctgc cacctcactc gcctgcaagc ccggtcgccc gtgtccatga actcgatggg
     6421 caggtacttc tcctcggcgt gggacacgat gccaacacga cgctgcatct tgccgagttg
     6481 atggcaaagg ttccctatgg ggtgccgaga cactgcacca ttcttcagga tggcaagttg
     6541 gtacgcgtcg attatctcga gaatgaccac tgctgtgagc gctttgcctt ggcggacagg
     6601 tggctcaagg agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt tgctcggttg
     6661 atccgctccc gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga tccgttgatc
     6721 ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca gtcgattggc
     6781 tgagctcatg agcggagaac gagatgacgt tggaggggca aggtcgcgct gattgctggg
     6841 gcaacacgtg gagcggatcg gggattgtct ttcttcagct cgctgatgat atgctgacgc
     6901 tcaatgccgt ttggcctccg actaacgaaa atcccgcatt tggacggctg atccgattgg
     6961 cacggcggac ggcgaatggc ggagcagacg ctcgtccggg ggcaatgaga tatgaaaaag
     7021 cctgaactca ccgcgacgta tcgggccctg gccagctagc tagagtcgac ctgcaggtcc
     7081 ccggggatcg gtcttgcctt gctcgtcggt gatgtacttc accagctccg cgaagtcgct
     7141 cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg cgcacccccc ggccgtttta
     7201 gcggctaaaa aagtcatggc tctgccctcg ggcggaccac gcccatcatg accttgccaa
     7261 gctcgtcctg cttctcttcg atcttcgcca gcagggcgag gatcgtggca tcaccgaacc
     7321 gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag gccgcccagg cggcccaggt
     7381 cgccattgat gcgggccagc tcgcggacgt gctcatagtc cacgacgccc gtgattttgt
     7441 agccctggcc gacggccagc aggtaggccg acaggctcat gccggccgcc gccgcctttt
     7501 cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg ctgcccttcc
     7561 tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg gcggtagccg
     7621 gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa gggacagtga
     7681 agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc tgacgccgtt
     7741 ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa
     7801 aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat gcagcggaaa
     7861 agatccgtcg acctgcaggc atgcaagctc tagcgattcc agacgtcccg aaggcgtggc
     7921 gcggcttccc cgtgccggag caatcgccct gggtgggtta cacgacgccc ctctatggcc
     7981 cgtactgacg gacacaccga agccccggcg gcaaccctca gcggatgccc cggggcttca
     8041 cgttttccca ggtcagaagc ggttttcggg agtagtgccc caactggggt aacctttgag
     8101 ttctctcagt tgggggcgta gggtcgccga catgacacaa ggggttgtga ccggggtgga
     8161 cacgtacgcg ggtgcttacg accgtcagtc gcgcgagcgc gaaaattcga gcgcagcaag
     8221 cccagcgaca cagcgtagcg ccaacgaaga caaggcggcc gaccttcagc gcgaagtcga
     8281 gcgcgacggg ggccggttca ggttcgtcgg gcatttcagc gaagcgccgg gcacgtcggc
     8341 gttcgggacg gcggagcgcc cggagttcga acgcatcctg aacgaatgcc gcgccgggcg
     8401 gctcaacatg atcattgtct atgacgtgtc gcgcttctcg cgcctgaagg tcatggacgc
     8461 gattccgatt gtctcggaat tgctcgccct gggcgtgacg attgtttcca ctcaggaagg
     8521 cgtcttccgg cagggaaacg tcatggacct gattcacctg attatgcggc tcgacgcgtc
     8581 gcacaaagaa tcttcgctga agtcggcgaa gattctcgac acgaagaacc ttcagcgcga
     8641 attgggcggg tacgtcggcg ggaaggcgcc ttacggcttc gagcttgttt cggagacgaa
     8701 ggagatcacg cgcaacggcc gaatggtcaa tgtcgtcatc aacaagcttg cgcactcgac
     8761 cactcccctt accggaccct tcgagttcga gcccgacgta atccggtggt ggtggcgtga
     8821 gatcaagacg cacaaacacc ttcccttcaa gccgggcagt caagccgcca ttcacccggg
     8881 cagcatcacg gggctttgta agcgcatgga cgctgacgcc gtgccgaccc ggggcgagac
     8941 gattgggaag aagaccgctt caagcgcctg ggacccggca accgttatgc gaatccttcg
     9001 ggacccgcgt attgcgggct tcgccgctga ggtgatctac aagaagaagc cggacggcac
     9061 gccgaccacg aagattgagg gttaccgcat tcagcgcgac ccgatcacgc tccggccggt
     9121 cgagcttgat tgcggaccga tcatcgagcc cgctgagtgg tatgagcttc aggcgtggtt
     9181 ggacggcagg gggcgcggca aggggctttc ccgggggcaa gccattctgt ccgccatgga
     9241 caagctgtac tgcgagtgtg gcgccgtcat gacttcgaag cgcggggaag aatcgatcaa
     9301 ggactcttac cgctgccgtc gccggaaggt ggtcgacccg tccgcacctg ggcagcacga
     9361 aggcacgtgc aacgtcagca tggcggcact cgacaagttc gttgcggaac gcatcttcaa
     9421 caagatcagg cacgccgaag gcgacgaaga gacgttggcg cttctgtggg aagccgcccg
     9481 acgcttcggc aagctcactg aggcgcctga gaagagcggc gaacgggcga accttgttgc
     9541 ggagcgcgcc gacgccctga acgcccttga agagctgtac gaagaccgcg cggcaggcgc
     9601 gtacgacgga cccgttggca ggaagcactt ccggaagcaa caggcagcgc tgacgctccg
     9661 gcagcaaggg gcggaagagc ggcttgccga acttgaagcc gccgaagccc cgaagcttcc
     9721 ccttgaccaa tggttccccg aagacgccga cgctgacccg accggcccta agtcgtggtg
     9781 ggggcgcgcg tcagtagacg acaagcgcgt gttcgtcggg ctcttcgtag acaagatcgt
     9841 tgtcacgaag tcgactacgg gcagggggca gggaacgccc atcgagaagc gcgcttcgat
     9901 cacgtgggcg aagccgccga ccgacgacga cgaagacgac gcccaggacg gcacggaaga
     9961 cgtagcggcg tagcgagaca cccgggaagc ctg
//
