pCMV-NP868R vector (V015595)

LOCUS       V015595                 6757 bp    DNA     circular SYN 17-APR-2019
DEFINITION  Exported.
ACCESSION   V015595
VERSION     V015595
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Philip AA, Agbemabiese CA, Heaton HE, Shmulevitz M, Patton JT.
  TITLE     Generation of Recombinant Rotavirus Expressing UnaG Fluorescent
            Protein Using the RNA Capping Enzyme of African Swine Fever Virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Philip AA, Agbemabiese CA, Heaton HE, Shmulevitz M, Patton JT.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2018) Biology, Indiana University, Simon Hall
            220D, Bloomington, IN 47405, USA
REFERENCE   3  (bases 1 to 6757)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-APR-2018) Biology, Indiana University, Simon Hall 220D,
            Bloomington, IN 47405, USA"
FEATURES             Location/Qualifiers
     source          1..6757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        620..638
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    651..666
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             680..3283
                     /note="mRNA-capping enzyme from African swine fever virus
                     (strain Badajoz 1971 Vero-adapted). Accession#: P32094"
                     /label="mRNA-capping enzyme"
     polyA_signal    3551..3672
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3679..4134)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4161..4263
                     /label="AmpR promoter"
     promoter        4267..4624
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4659..5450
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5685..5732
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6061..6649
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"