LOCUS       V015595                 6757 bp    DNA     circular SYN 17-APR-2019
DEFINITION  Exported.
ACCESSION   V015595
VERSION     V015595
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Philip AA, Agbemabiese CA, Heaton HE, Shmulevitz M, Patton JT.
  TITLE     Generation of Recombinant Rotavirus Expressing UnaG Fluorescent
            Protein Using the RNA Capping Enzyme of African Swine Fever Virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Philip AA, Agbemabiese CA, Heaton HE, Shmulevitz M, Patton JT.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2018) Biology, Indiana University, Simon Hall
            220D, Bloomington, IN 47405, USA
REFERENCE   3  (bases 1 to 6757)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-APR-2018) Biology, Indiana University, Simon Hall 220D,
            Bloomington, IN 47405, USA"
FEATURES             Location/Qualifiers
     source          1..6757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        620..638
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    651..666
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             680..3283
                     /note="mRNA-capping enzyme from African swine fever virus
                     (strain Badajoz 1971 Vero-adapted). Accession#: P32094"
                     /label="mRNA-capping enzyme"
     polyA_signal    3551..3672
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3679..4134)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4161..4263
                     /label="AmpR promoter"
     promoter        4267..4624
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4659..5450
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5685..5732
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6061..6649
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcggtggcgg ccgcccacca tggcttcttt agataattta gtggcacgat atcagaggtg
      721 ctttaatgac cagtctctta aaaatagtac tattgaactt gaaatacgtt ttcaacagat
      781 aaatttttta ttattcaaaa ccgtatatga ggcacttgtg gcacaagaga tccctagcac
      841 catctcccac agcatccgct gcatcaaaaa agttcaccat gaaaaccact gccgggaaaa
      901 aattttgccg tcggaaaatc tttacttcaa aaaacagcct ctcatgtttt ttaagttttc
      961 agagcctgca tctctgggct gtaaggtctc gctggccatc gagcagccca ttcgtaaatt
     1021 tatcttggac tcctccgttc tcgttcggct caaaaatcgt acgacctttc gggtatctga
     1081 actttggaaa atagagctta ccattgtaaa gcagctgatg ggaagcgagg tctctgcaaa
     1141 acttgccgct ttcaaaacgc ttctgtttga caccccagag caacaaacga caaaaaatat
     1201 gatgacgtta ataaacccag atgacgaata tctttacgaa atagaaatag agtatacagg
     1261 aaagcccgaa tccctaacgg cggcagatgt tataaaaatt aaaaacacgg tgttgacact
     1321 tatttctccg aaccatttaa tgctaacagc ctaccaccag gccattgaat tcattgcctc
     1381 ccatatactg tcctcagaaa tccttcttgc tcgtattaag agcgggaagt gggggcttaa
     1441 acgcctcctc ccccaggtga aatccatgac caaagcggat tacatgaaat tttatccgcc
     1501 cgttggctac tatgtaacgg acaaagcaga tggaattaga ggcatcgccg tcattcagga
     1561 cacgcaaatt tatgtggttg cagaccagct atacagccta ggtaccaccg gcattgaacc
     1621 ccttaaacca accattttgg atggtgaatt tatgcctgaa aaaaaagaat tttatgggtt
     1681 tgacgtcatc atgtatgagg gcaatctgtt gacgcaacag gggtttgaaa caagaattga
     1741 gtctttaagc aagggcatta aagtcttaca agcgtttaac ataaaagcag aaatgaagcc
     1801 ctttatttcg ctaacaagtg cagatcccaa cgtgctcctc aaaaactttg aaagcatttt
     1861 taagaaaaaa actcgcccat attctattga tggcatcatt ttagtagaac ctggcaattc
     1921 ttatctaaat acaaatactt ttaagtggaa gcccacctgg gataacacat tagacttttt
     1981 ggtgcgaaaa tgtccggaga gtttaaacgt gccagagtac gcgcccaaaa aagggttttc
     2041 cctgcatcta ctatttgtag gcatctccgg agagcttttt aaaaaattag cgctaaattg
     2101 gtgtccagga tatacgaaac tattccccgt tacacagcgc aaccaaaact actttccagt
     2161 acagtttcag ccatcggatt ttccactggc atttctttat taccacccag atacctcgtc
     2221 attttctaat atagatggaa aggtccttga aatgcgttgt cttaagagag aaattaatta
     2281 cgtcagatgg gaaattgtaa aaatccggga ggataggcag caggatctta aaaccggcgg
     2341 gtattttggc aatgatttca aaacagccga actcacatgg cttaactata tggatccctt
     2401 ttcctttgag gaactggcaa agggcccttc tggaatgtac ttcgccggtg ccaaaaccgg
     2461 catataccgc gctcaaacag cacttatttc ctttattaaa caagaaatca tccaaaaaat
     2521 aagtcaccaa tcctgggtta tcgatcttgg aataggaaaa gggcaggacc taggacgtta
     2581 cctggacgca ggggtaaggc atcttgttgg aatcgataag gaccaaaccg cgctcgcgga
     2641 gcttgtttat cgaaaatttt cgcatgctac aacccgacag cacaagcacg ctaccaacat
     2701 ttacgtgttg catcaagacc tcgcagagcc tgcgaaggaa atcagcgaaa aggtacacca
     2761 aatttacggg tttcccaagg agggagcttc ttccattgtt agcaacctgt ttattcacta
     2821 tcttatgaaa aacacgcagc aggtggaaaa cctggccgtt ctgtgccata agcttcttca
     2881 gccgggggga atggtgtggt ttaccaccat gttgggagaa caggtcttag aattacttca
     2941 tgaaaataga atagagctca atgaagtatg ggaggcacgt gaaaacgagg tggtcaaatt
     3001 tgctattaaa cgtctcttta aagaggatat attacaggaa actgggcaag aaattggagt
     3061 cctgttaccc ttcagcaatg gcgacttcta caatgaatat cttgtgaaca cagcgttttt
     3121 aattaaaata tttaaacatc acggcttttc cctagttcaa aagcagtcct ttaaggactg
     3181 gattccagaa tttcaaaact ttagtaaaag tttgtataaa attcttacag aagccgataa
     3241 aacttggaca agcctttttg ggtttatttg tctgcgcaaa aattaacgat cgcccttccc
     3301 aacagttgcg cagcctgaat ggcgaatgga gatccaattt ttaagtgtat aatgtgttaa
     3361 actactgatt ctaattgttt gtgtatttta gattcacagt cccaaggctc atttcaggcc
     3421 cctcagtcct cacagtctgt tcatgatcat aatcagccat accacatttg tagaggtttt
     3481 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     3541 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3601 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3661 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     3721 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     3781 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3841 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3901 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3961 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     4021 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     4081 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     4141 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4201 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4261 agaatcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4321 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4381 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4441 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4501 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4561 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4621 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     4681 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     4741 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     4801 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     4861 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     4921 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     4981 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5041 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5101 tggaagccgg tcttgtcgat caggatgatc tggacgaaga acatcagggg ctcgcgccag
     5161 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     5221 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5281 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5341 ttgctgaaga acttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5401 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5461 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     5521 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     5581 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     5641 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     5701 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     5761 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     5821 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     5881 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     5941 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6001 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6061 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6121 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6181 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     6241 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6301 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6361 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6421 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6481 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6541 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6601 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6661 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6721 gttatcccct gattctgtgg ataaccgtat taccgcc
//
