PCM28 vector (V015587)

LOCUS       V015587                 5594 bp    DNA     circular SYN 16-MAY-2020
DEFINITION  Exported.
ACCESSION   V015587
VERSION     V015587
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   DSouza R, Nikolich MP, Horswill A, Goglin K, Fouts DE.
  TITLE     PCM28: Staphylococcus aureus shuttle vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   DSouza R, Nikolich MP, Horswill A, Goglin K, Fouts DE.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2020) Genomic Medicine, J. Craig Venter Institute,
            9605 Medical Centre Dr, Rockville, MD 20850, USA
REFERENCE   3  (bases 1 to 5594)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.7
            Coverage              :: >100X
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-JAN-2020) Genomic Medicine, J. Craig Venter Institute, 9605
            Medical Centre Dr, Rockville, MD 20850, USA"
FEATURES             Location/Qualifiers
     source          1..5594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             560..574
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             592..825
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /db_xref="SEED:fig|6666666.501693.peg.2"
                     /protein_id="QJS05070.1"
                     /translation="MLKCFIILTLYKHYTFVIQILTLLGTIKNHENFNLHVTGQCLKKS
                     TLNLLKFLFVELEYIYLAHICFLKACMPAGRL"
     primer_bind     complement(863..879)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(887..903)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(911..941)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(956..977)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1265..1853)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2027..2884)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2885..2989)
                     /label="AmpR promoter"
     primer_bind     3463..3479
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3546..3686)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /db_xref="SEED:fig|6666666.501693.peg.4"
                     /protein_id="QJS05072.1"
                     /translation="MIAKNAIPIQNHIPIIDNHITVIKPLLFNKLYHKKYLNFKCLYFE
                     F"
     CDS             complement(3756..3947)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /db_xref="SEED:fig|6666666.501693.peg.5"
                     /protein_id="QJS05073.1"
                     /translation="MKKVFILQLQIHILLFLNRLLLFRFFIPIALLTLSLGTLNNPKTC
                     RMVGLIAHAMPTFVCKFS"
     CDS             complement(4016..4663)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(4797..5057)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /db_xref="SEED:fig|6666666.501693.peg.7"
                     /protein_id="QJS05075.1"
                     /translation="MFFTSHLKRYINRYEKATFFALKTSHTNNLRVTSLAGNSYPYYQD
                     KKEKDFSLRSNPLKKHKRPHFLMWSFILQLKHPLVQQTKIG"