LOCUS       V015587                 5594 bp    DNA     circular SYN 16-MAY-2020
DEFINITION  Exported.
ACCESSION   V015587
VERSION     V015587
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   DSouza R, Nikolich MP, Horswill A, Goglin K, Fouts DE.
  TITLE     PCM28: Staphylococcus aureus shuttle vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   DSouza R, Nikolich MP, Horswill A, Goglin K, Fouts DE.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2020) Genomic Medicine, J. Craig Venter Institute,
            9605 Medical Centre Dr, Rockville, MD 20850, USA
REFERENCE   3  (bases 1 to 5594)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.7
            Coverage              :: >100X
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-JAN-2020) Genomic Medicine, J. Craig Venter Institute, 9605
            Medical Centre Dr, Rockville, MD 20850, USA"
FEATURES             Location/Qualifiers
     source          1..5594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             560..574
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             592..825
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /db_xref="SEED:fig|6666666.501693.peg.2"
                     /protein_id="QJS05070.1"
                     /translation="MLKCFIILTLYKHYTFVIQILTLLGTIKNHENFNLHVTGQCLKKS
                     TLNLLKFLFVELEYIYLAHICFLKACMPAGRL"
     primer_bind     complement(863..879)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(887..903)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(911..941)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(956..977)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1265..1853)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2027..2884)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2885..2989)
                     /label="AmpR promoter"
     primer_bind     3463..3479
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3546..3686)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /db_xref="SEED:fig|6666666.501693.peg.4"
                     /protein_id="QJS05072.1"
                     /translation="MIAKNAIPIQNHIPIIDNHITVIKPLLFNKLYHKKYLNFKCLYFE
                     F"
     CDS             complement(3756..3947)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /db_xref="SEED:fig|6666666.501693.peg.5"
                     /protein_id="QJS05073.1"
                     /translation="MKKVFILQLQIHILLFLNRLLLFRFFIPIALLTLSLGTLNNPKTC
                     RMVGLIAHAMPTFVCKFS"
     CDS             complement(4016..4663)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(4797..5057)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /label="hypothetical protein"
                     /db_xref="SEED:fig|6666666.501693.peg.7"
                     /protein_id="QJS05075.1"
                     /translation="MFFTSHLKRYINRYEKATFFALKTSHTNNLRVTSLAGNSYPYYQD
                     KKEKDFSLRSNPLKKHKRPHFLMWSFILQLKHPLVQQTKIG"
ORIGIN
        1 ttaattgcag taaataaatc gtatttcaca gataaaagat attatattag ccaacaagaa
       61 tggttagatt tatggcgtga tgtaacgggc atttcagaaa taacacaagt tcaagttcaa
      121 aaaataagac aaaataataa taaagaatta tatgaaatgg ctaagtattc tggtaaagat
      181 agtgattatt taataaatca aaaagtcttt gatgcatttt ataaatcact taaaggtaaa
      241 caggtattag tttattcagg attatttaaa gaggctaaaa agaaattaaa aaatggggat
      301 ttagattact taaaagaaat tgatccaacc gaatatatct atcaaatttt ttatatttgg
      361 aaacaaaaag agtatttagc tagtgaactt tatgacttaa cagaacaaga aaaaagagaa
      421 attaatcaca aaatgataga cgaaatcgag gaagaacaat aacaaaatat aagtgctaac
      481 agctgacctc ccgataacac catgtagtta ttgggaggtc agctgttgaa ttatgcacga
      541 gtattttaaa agttattgtg atgacgacga taaacgatta tcaaaagtat aatgttaaaa
      601 tgctttatta tactaacgtt atataaacat tatactttcg ttatacaaat tttaaccctg
      661 ttaggaacta taaaaaatca tgaaaatttt aatttgcatg taactgggca gtgtcttaaa
      721 aaatcgacac tgaatttgct caaatttttg tttgtagaat tagaatatat ttatttggct
      781 catatttgct ttttaaaagc ttgcatgcct gcaggtcgac tctagaggat ccgctagcat
      841 tcgatcatga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
      901 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      961 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1021 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1141 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1201 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1261 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1321 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1381 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1441 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1501 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1561 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1621 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1681 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1741 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1801 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1861 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1921 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1981 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2041 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2101 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2161 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2221 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2281 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2341 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2401 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2461 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2521 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2581 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2641 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2701 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2761 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2821 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2881 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2941 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3001 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3061 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     3121 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     3181 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     3241 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     3301 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     3361 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     3421 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     3481 ccaagcttct gtaggttttt aggcataaaa ctatatgatt tacccctaaa tctttaaaat
     3541 gccccttaaa attcaaaata aaggcattta aaatttaaat atttcttgtg ataaagtttg
     3601 ttaaaaagga gtggttttat gactgttatg tggttatcga ttataggtat gtggttttgt
     3661 attggaatgg cattttttgc tatcaaggtt attaaaaata aaaattagac cacgcattta
     3721 tgccgagaaa atttattgtg cgttgagaag aacccttaac taaacttgca gacgaatgtc
     3781 ggcatagcgt gagctattaa gccgaccatt cgacaagttt tgggattgtt aagggttccg
     3841 aggctcaacg tcaataaagc aattggaata aagaagcgaa aaaggagaag tcggttcaga
     3901 aaaagaagga tatggatctg gagctgtaat ataaaaacct tcttcaacta acggggcagg
     3961 ttagtgacat tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa
     4021 gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat
     4081 aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt
     4141 tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat
     4201 ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag
     4261 gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt
     4321 ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt
     4381 tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc
     4441 cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga
     4501 aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa
     4561 tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct
     4621 cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa
     4681 tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc
     4741 ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat
     4801 ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaaaga ccacattaaa
     4861 aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc cttttctttc
     4921 ttatcttgat aataagggta actattgccg gcgaggctag ttacccttaa gttattggta
     4981 tgactggttt taagcgcaaa aaaagttgct ttttcgtacc tattaatgta tcgttttaaa
     5041 tgactagtaa aaaacataca tagaaagggg aaaaagcaac tttttttatt gtcatagttt
     5101 gtgaaaacta agttgttttt atgtgttata acatggaaaa gtatactgag aaaaaacaaa
     5161 gaaatcaagt atttcagaaa tttattaaac gtcatattgg agagaatcaa atggatttag
     5221 ttgaagattg caatacattt ctgtcttttg tagctgataa aactttagaa aaacagaaat
     5281 tatataaagc taattcttgt aaaaatcgat tttgtcctgt ctgtgcttgg agaaaagcta
     5341 gaaaagatgc attgggttta tctttgatga tgcaatatat taagcagcaa gagaaaaagg
     5401 agtttatctt tttaactttg actacaccta atgtaatgag tgatgaatta gaaaatgaaa
     5461 taaaacgtta taataattct tttagaaaac ttataaagag aaaaaaagta ggtagtgtta
     5521 taaagggata tgttcgtaag ttagagatta catataataa aaaaagagat gattataatc
     5581 ctcattttca tgtg
//
