pMJA297 vector (V015569)

LOCUS       V015569                 7867 bp    DNA     circular SYN 12-JUN-2019
DEFINITION  Exported.
ACCESSION   V015569
VERSION     V015569
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Wang R, Ghumra A, Fairclough L, Alcocer M.
  TITLE     Nut lipids are involved in the intrinsic allergenicity of nut
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Wang R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2019) School of Biosciences, University of
            Nottingham, Babs Building, Sutton Bonington LE12 5RD, UK
REFERENCE   3  (bases 1 to 7867)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-APR-2019) School of Biosciences, University of Nottingham, Babs
            Building, Sutton Bonington LE12 5RD, UK"
FEATURES             Location/Qualifiers
     source          1..7867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(570..625)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             complement(782..1315)
                     /gene="TRBC2"
                     /label="T cell receptor beta constant 2"
                     /note="T cell receptor beta constant 2 from Homo sapiens.
                     Accession#: A0A5B9"
     promoter        complement(1974..2177)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2178..2481)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             3420..3839
                     /gene="TRAC"
                     /label="T cell receptor alpha chain constant"
                     /note="T cell receptor alpha chain constant from Homo
                     sapiens. Accession#: P01848"
     polyA_signal    3880..4104
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4150..4578
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4592..4922
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4970..5017
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5036..5407
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5540..5673
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5710..5726)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5734..5750)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5758..5788)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5803..5824)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6112..6700)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6874..7731)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7732..7836)
                     /label="AmpR promoter"