Basic Vector Information
- Vector Name:
- pMJA297
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7867 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- Wang R, Ghumra A, Fairclough L, Alcocer M.
- Promoter:
- CMV
pMJA297 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMJA297 vector Sequence
LOCUS 62056_17000 7867 bp DNA circular SYN 12-JUN-2019
DEFINITION Mammalian expression vector pMJA297, complete sequence.
ACCESSION MK764037
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7867)
AUTHORS Wang R, Ghumra A, Fairclough L, Alcocer M.
TITLE Nut lipids are involved in the intrinsic allergenicity of nut
proteins
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7867)
AUTHORS Wang R.
TITLE Direct Submission
JOURNAL Submitted (05-APR-2019) School of Biosciences, University of
Nottingham, Babs Building, Sutton Bonington LE12 5RD, UK
REFERENCE 3 (bases 1 to 7867)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(05-APR-2019) School of Biosciences, University of Nottingham, Babs
Building, Sutton Bonington LE12 5RD, UK"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..7867
/mol_type="other DNA"
/organism="synthetic DNA construct"
polyA_signal complement(570..625)
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
CDS complement(782..1315)
/gene="TRBC2"
/label=TRBC2
/note="T cell receptor beta constant 2 from Homo sapiens.
Accession#: A0A5B9"
promoter complement(1974..2177)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer complement(2178..2481)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
CDS 3420..3839
/gene="TRAC"
/label=TRAC
/note="T cell receptor alpha chain constant from Homo
sapiens. Accession#: P01848"
polyA_signal 3880..4104
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4150..4578
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4592..4922
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 4970..5017
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 5036..5407
/label=BleoR
/note="antibiotic-binding protein"
polyA_signal 5540..5673
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5710..5726)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(5734..5750)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5758..5788)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(5803..5824)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6112..6700)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6874..7731)
/label=AmpR
/note="beta-lactamase"
promoter complement(7732..7836)
/label=AmpR promoter
This page is informational only.