LOCUS       V015569                 7867 bp    DNA     circular SYN 12-JUN-2019
DEFINITION  Exported.
ACCESSION   V015569
VERSION     V015569
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Wang R, Ghumra A, Fairclough L, Alcocer M.
  TITLE     Nut lipids are involved in the intrinsic allergenicity of nut
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Wang R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2019) School of Biosciences, University of
            Nottingham, Babs Building, Sutton Bonington LE12 5RD, UK
REFERENCE   3  (bases 1 to 7867)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-APR-2019) School of Biosciences, University of Nottingham, Babs
            Building, Sutton Bonington LE12 5RD, UK"
FEATURES             Location/Qualifiers
     source          1..7867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(570..625)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             complement(782..1315)
                     /gene="TRBC2"
                     /label="T cell receptor beta constant 2"
                     /note="T cell receptor beta constant 2 from Homo sapiens.
                     Accession#: A0A5B9"
     promoter        complement(1974..2177)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(2178..2481)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             3420..3839
                     /gene="TRAC"
                     /label="T cell receptor alpha chain constant"
                     /note="T cell receptor alpha chain constant from Homo
                     sapiens. Accession#: P01848"
     polyA_signal    3880..4104
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4150..4578
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4592..4922
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4970..5017
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             5036..5407
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5540..5673
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5710..5726)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5734..5750)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5758..5788)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5803..5824)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6112..6700)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6874..7731)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7732..7836)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagtaagaga agagggacag ctatgactgg gagtagtcag gagaggagga
      301 aaaatctggc tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca
      361 caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat
      421 ggacagcagg gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc
      481 agccagcata tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
      541 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
      601 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
      661 tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg
      721 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttgaattcct
      781 agcctctgga atcctttctc ttgaccatgg ccatcagcac gagggcactg accagcacgg
      841 catacaaggt ggccttccct agcaagatct catagaggat ggtggcagac aggacccctt
      901 gctggtaaga ctcggaggtg aagccacagt ctgctctacc ccaggcctcg gcgctgacga
      961 tctgggtgac aggtttggcc ctatcctggg tccactcgtc attctccgag agcccgtaga
     1021 actggacttg acagcggaag tggttgcggg ggttctgcca gaaggtggcc gagaccctca
     1081 ggcggctgct caggcagtat ctggagtcat tgagggcggg ctgctccttg aggggctgcg
     1141 ggtctgtgct gaccccactg tgcacctcct tcccattcac ccaccagctc agctccacgt
     1201 ggtcggggta gaagcctgtg gccaggcaca ccagtgtggc cttttgggtg tgggagatct
     1261 ctgcttctga tggctcaaac acagcgacct cgggtgggaa cacgtttttc aggtcctcga
     1321 gcactgtcag ccgggtgcct gggccaaaat actgcgtatc caccgagccc ccctgagggc
     1381 tgctggcaca gaagtacaca gatgtctgag agggtacagc agacgccaac gtgaggggga
     1441 aatcatctgt gtttgctctg gagacactat aaccatcagg gacttctcct ttgccagtgg
     1501 tacctgcagt atttgaataa tggatgagcc ttagccccag tcctagatct tgtctatacc
     1561 agtacatggc attatgtctc atatcctggg tacatctcag tgtcatgcgc cgtcctgctg
     1621 ccaggatctg agatgttggt gcctgggtga tcccagcaat cactggacct acagagaagg
     1681 agaacaaagc caatgctgca gcctcaatgg gaaggctctg ggtcttgaaa tctacacatg
     1741 ggccctgccc aggacttacc tgcccacagg agagaaaagg ccacacagca caggagcctg
     1801 attctcatga cagcatcccc ttgttcaggg tccaggactt ctgggggaac tatttggagg
     1861 tttggatctg tagtctccta ataggctctg ctcgacaagc ttatcgatgc ggccgcgcta
     1921 gcacgcgtca gctgactaga ggatccctag cggatctgac ggttcactaa accagctctg
     1981 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     2041 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     2101 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     2161 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     2221 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     2281 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     2341 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     2401 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     2461 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     2521 attgattact attaataact actcgagtat gtcgaggtgg cgtgtacggt gggaggccta
     2581 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg aggtaccacc ggtcatatgc
     2641 cgcgggagcc cagatcctgt gtgtgtgtgt gtgtgcttgt gcacatctgt tctctgattg
     2701 gctgactgta cttcctgaga gtccctgttt aatgttacaa gaaacagctg atgtggggag
     2761 ttttccttct ttatgtttcc atgaagatgg gaggtaagtc tcaatctaat agcaaatgct
     2821 gctgggagtt ttcaaaacga ttttctttca gctaaatgat tgccatttct gacactcgta
     2881 acaaaagtgt ttcttaatag atattttctt tatgcggtgc taattgttat tggatatcaa
     2941 ataataacga ctttgctgcc atttatttct aagaatctct ccttaatggc aattcttttg
     3001 tgaccacatg gagacatcgt ttttaatgat aatgggtttc caggagcgct gccctagtaa
     3061 cagtcatgta tcttgtctta ggcactacag gacaaaacat tgaccagccc actgagatga
     3121 cagctacgga aggtgccatt gtccagatca actgcacgta ccagacatct gggttcaacg
     3181 ggctgttctg gtaccagcaa catgctggcg aagcacccac atttctgtct tacaatgttc
     3241 tggatggttt ggaggagaaa ggtcgttttt cttcattcct tagtcggtct aaagggtaca
     3301 gttacctcct tttgaaggag ctccagatga aagactctgc ctcttacctc tgtgcagggg
     3361 tggatagcag ctataaattg atcttcggag caggaacaag actatttgtt aaagcaaata
     3421 tccagaaccc tgaccctgcc gtgtaccagc tgagagactc taaatccagt gacaagtctg
     3481 tctgcctatt caccgatttt gattctcaaa caaatgtgtc acaaagtaag gattctgatg
     3541 tgtatatcac agacaaaact gtgctagaca tgaggtctat ggacttcaag agcaacagtg
     3601 ctgtggcctg gagcaacaaa tctgactttg catgtgcaaa cgccttcaac aacagcatta
     3661 ttccagaaga caccttcttc cccagcccag aaagttcctg tgatgtcaag ctggtcgaga
     3721 aaagctttga aacagatacg aacctaaact ttcaaaacct gtcagtgatt gggttccgaa
     3781 tcctcctcct gaaagtggcc gggtttaatc tgctcatgac gctgcggctg tggtccagct
     3841 gatctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3901 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3961 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4021 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4081 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     4141 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4201 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4261 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt
     4321 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4381 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4441 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4501 ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac
     4561 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4621 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     4681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4741 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     4801 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     4861 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     4921 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     4981 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     5041 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     5101 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     5161 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     5221 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     5281 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     5341 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     5401 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5461 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5521 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     5581 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5641 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     5701 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5761 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5821 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5881 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5941 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6001 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6061 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6121 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6181 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6241 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6301 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6361 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6421 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6481 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6541 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6601 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6661 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6721 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6781 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6841 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6901 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6961 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7021 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7081 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7141 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7201 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7261 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7321 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7381 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     7441 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     7501 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     7561 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     7621 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     7681 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     7741 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     7801 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     7861 tgacgtc
//
