Basic Vector Information
- Vector Name:
- yellowCC
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7388 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Li Z.
- Promoter:
- dU6-3
yellowCC vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
yellowCC vector Sequence
LOCUS 62056_23515 7388 bp DNA circular SYN 21-APR-2021
DEFINITION Cloning vector yellowCC, complete sequence.
ACCESSION MW770349
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7388)
AUTHORS Li Z.
TITLE Direct Submission
JOURNAL Submitted (18-MAR-2021) Cell and Developmental Biology, University
of California, San Diego, 9500 Gilman Drive Tata Hall 5401, San
Diego, CA 92122, USA
REFERENCE 2 (bases 1 to 7388)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(18-MAR-2021) Cell and Developmental Biology, University of
California, San Diego, 9500 Gilman Drive Tata Hall 5401, San Diego,
CA 92122, USA"
FEATURES Location/Qualifiers
source 1..7388
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(127..715)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(889..1746)
/label=AmpR
/note="beta-lactamase"
promoter complement(1747..1851)
/label=AmpR promoter
regulatory 1944..3049
/label=Dmel yellow left homologous arm
/note="Dmel yellow left homologous arm"
/regulatory_class="locus_control_region"
CDS 3270..3944
/label=DsRed2
/note="improved tetrameric variant of DsRed fluorescent
protein"
CDS 3954..3974
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 4115..4236
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 4239..4659
/label=dU6-3 promoter
/note="RNA polymerase III promoter for Drosophila U6-3
snRNA (Port et al., 2014)"
misc_RNA 4679..4754
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
regulatory 4758..4985
/label=U6 3'UTR
/note="U6 3'UTR"
/regulatory_class="terminator"
polyA_signal complement(5029..5158)
/label=OpIE-2 poly(A) signal
/note="baculovirus polyadenylation signal"
CDS complement(5162..5188)
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS complement(5189..5215)
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS complement(5216..5242)
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS complement(5306..6019)
/label=mCerulean
/note="enhanced monomeric variant of CFP (Rizzo et al.,
2004)"
CDS complement(6032..6052)
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
regulatory complement(6071..6277)
/label=3XP3
/note="3XP3"
/regulatory_class="promoter"
protein_bind complement(6287..6320)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
CDS 7170..7181
/label=Factor Xa site
/note="Factor Xa recognition and cleavage site"
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