LOCUS       62056_23515        7388 bp DNA     circular SYN 21-APR-2021
DEFINITION  Cloning vector yellowCC, complete sequence.
ACCESSION   MW770349
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Li Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2021) Cell and Developmental Biology, University 
            of California, San Diego, 9500 Gilman Drive Tata Hall 5401, San 
            Diego, CA 92122, USA
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-MAR-2021) Cell and Developmental Biology, University of 
            California, San Diego, 9500 Gilman Drive Tata Hall 5401, San Diego, 
            CA 92122, USA"
FEATURES             Location/Qualifiers
     source          1..7388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(127..715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(889..1746)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1747..1851)
                     /label=AmpR promoter
     regulatory      1944..3049
                     /label=Dmel yellow left homologous arm
                     /note="Dmel yellow left homologous arm"
                     /regulatory_class="locus_control_region"
     CDS             3270..3944
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     CDS             3954..3974
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4115..4236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4239..4659
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3
                     snRNA (Port et al., 2014)"
     misc_RNA        4679..4754
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      4758..4985
                     /label=U6 3'UTR
                     /note="U6 3'UTR"
                     /regulatory_class="terminator"
     polyA_signal    complement(5029..5158)
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     CDS             complement(5162..5188)
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(5189..5215)
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(5216..5242)
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(5306..6019)
                     /label=mCerulean
                     /note="enhanced monomeric variant of CFP (Rizzo et al.,
                     2004)"
     CDS             complement(6032..6052)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     regulatory      complement(6071..6277)
                     /label=3XP3
                     /note="3XP3"
                     /regulatory_class="promoter"
     protein_bind    complement(6287..6320)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7170..7181
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
ORIGIN
        1 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
       61 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      121 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      181 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      241 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      301 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      361 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      421 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      481 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      541 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      601 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      661 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      721 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
      781 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      841 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
      901 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
      961 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1021 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1081 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1141 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1201 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1261 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1321 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1381 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1441 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1501 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1561 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1621 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1681 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1741 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1801 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1861 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     1921 taggcgtatc acgaggccct ttcggcaaca acaggctaca gaatacctat aagtgacagt
     1981 tatggggtat gattaattat aaatattatc attgaccacc aatgctgggc tcaattggaa
     2041 aaactattct atgaagattt gagtaaataa attttgattt aaaaaagccc atggttatcg
     2101 cgacaactag ctacgggaca agattactgt ttaaaatcaa gtgtgaaata tcaaaatcaa
     2161 aatcggattc cgatcgggaa gttgtatccg attctgaaac taaaacacag aattgccaac
     2221 attttccgat atcgactcag ctcacgtatt tcatacagat tcattaggcc accagccatt
     2281 gaataatata ccccagtcaa ttgagctact cgatagttga tcaacttagc ttttgtcaac
     2341 gagtgaacgc ataaactact acatcaacga tatttgcggc ccattccaag ctaaaagttc
     2401 atcttaatta caaataagat tagaaaaaat atctgaatga aaaaaatgtt gagacatatt
     2461 tctttggaaa aggagaacct caagacagtc gaaaaaattg tttacaatga aaatgttgaa
     2521 aatcatgaag cagataaatc tgtcagttgc gaggttttag gactgaaaga gcacatgtca
     2581 aaatataaat ttgttcaaat actttatatt tgactgaatt agattgttat tttaaaagtt
     2641 atgaattaaa taaagattga aaggtgcatt atgctcaaat gtatatttat cgcaaccccc
     2701 ggttactttg taaagcaaaa acgcctggtt tgatttttaa gaagatgggt cggtaaatcg
     2761 ataaaagcta tattttctgg tcgttgcagt ctcactcgcc tgctataaaa acattaaaag
     2821 ttcccagaaa caataaatgt ctttaaattc aattaacgaa gaaataaaga aggaaaagaa
     2881 ctggagcgga aatcggtcga aatactgcca atggccacat atacatttaa cagcgatata
     2941 tggtatacat attgataatg atgtcagacg caattgcttc agacggctaa tgacatcgca
     3001 aattgcacgc aacttgcaat agtgccaatt atgactgaag tacatatagg tccatcatga
     3061 tggtcgactc tagaagtcga tccaacatgg cgacttgtcc catccccggc atgtttaaat
     3121 atactaatta ttcttgaact aattttaatc aaccgattta tctctcttcc gcaggtggcg
     3181 gaggttccgg tggaagcgga ggtagcggcg gatccgaggg ccgcggcagc ctgctgacct
     3241 gcggcgatgt ggaggagaac cccgggccca tggcctcctc cgagaacgtc atcaccgagt
     3301 tcatgcgctt caaggtgcgc atggagggca ccgtgaacgg ccacgagttc gagatcgagg
     3361 gcgagggcga gggccgcccc tacgagggcc acaacaccgt gaagctgaag gtgaccaagg
     3421 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg
     3481 tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct
     3541 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg acccaggact
     3601 cctccctgca ggacggctgc ttcatctaca aggtgaagtt catcggcgtg aacttcccct
     3661 ccgacggccc cgtgatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     3721 acccccgcga cggcgtgctg aagggcgaga cccacaaggc cctgaagctg aaggacggcg
     3781 gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg
     3841 gctactacta cgtggacgcc aagctggaca tcacctccca caacgaggac tacaccatcg
     3901 tggagcagta cgagcgcacc gagggccgcc accacctgtt cctgagatct cgacccaaga
     3961 aaaagcggaa ggtggaggac ccgtaagatc caccggatct agataactga tcataatcag
     4021 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     4081 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     4141 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     4201 tagttgtggt ttgtccaaac tcatcaatgt atcttacctt ttttgctcac ctgtgattgc
     4261 tcctactcaa atacaaaaac atcaaatttt ctgtcaataa agcatattta tttatattta
     4321 ttttacagga aagaattcct tttaaagtgt attttaacct ataatgaaaa acgattaaaa
     4381 aaaatacata aaataattcg aaaatttttg aatagcccag gttgataaaa attcatttca
     4441 tacgttttat aacttatgcc cctaagtatt ttttgaccat agtgtttcaa ttctacatta
     4501 attttacaga gtagaatgaa acgccaccta ctcagccaag aggcgaaaag gttagctcgc
     4561 caagcagaga gggcgccagt gctcactact ttttataatt ctcaacttct ttttccagac
     4621 tcagttcgta tatatagacc tattttcaat ttaacgtcga ctgaagtaca tatagccggt
     4681 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     4741 caccgagtcg gtgctttttt gcctacctgg agcctgagag ttgttcaata aaataaaaat
     4801 gtttcgtttt tttgctttcg ccagtattta ttatttttca tcaatatgta ttcaatttgg
     4861 tatgtattta gtaattgtaa tatatagaca atggttttcc gttgacgtac atacatctga
     4921 cgtgtgttta tttagacata atagttatgt tttcacatct ttttaatgtt cgcttaatgc
     4981 gtatgcattt agacataact tcgtatagca tacattatac gaagttatca cgcgcttgaa
     5041 aggagtgtgt aaatggacaa tgttgttaca ataaaagttg aaattatttt ataaaattta
     5101 ttaaaaacat atttaaacat aacatattgt attaaaacat gacaatacaa actaagattt
     5161 acgcgtaatc gggaacgtcg tatgggtagg cgtaatctgg cacatcgtag gggtaagcat
     5221 aatctggaac gtcgtatgga taggatccct ttttcttttt tgcctggccg gcctttttcg
     5281 tggccgccgg cctttttcca ctgcccttat acaactcatc catgcccaaa gttattcccg
     5341 cagctgttac aaactccagc aaaaccatat ggtcgcgttt ttcgttaggg tccttgctca
     5401 atttcgactg ggtggacagg taatggttgt ctggcagcaa taccgggccg tcgccgatcg
     5461 gagtattctg ctgataatgg tcggcgagct gtacgctgcc atcctctata ttacagttaa
     5521 gtccaaaatt ggctttaatt ccgttttttt gtttgtcagc cgtaatgtag acatttccgt
     5581 gaatggcatt gtactcgagc ttgtgaccca gaatgttacc gtcttcctta aaatcgattc
     5641 ccttcagttc gattcggttc accaaggtat cgccctcaaa ctttacttca gccctggttt
     5701 tatagttgcc gtcatccttg aagaatatcg tccgctcctg tacatagccc tcgggcatag
     5761 cacttttgaa aaaatcatgt tgcttcatat gatcagggta gcgtgcaaag cactggacac
     5821 cccagctcag ggttgtcacc aaggttggcc acggcactgg gagctttccg gtcgtacata
     5881 tgaacttgag tgtcaacttt ccataggtag catctccctc gccctcgcca ctgaccgaga
     5941 acttatgacc attcacatcg ccatccagtt cgaccaatat tggcactact cctgtgaaca
     6001 gctcttcgcc tttgctgact ccggatccgc caacttttcg ttttttcttg ggcatggtgg
     6061 cgaccggctt cgagccgatt gtttagcttg ttcagctgcg cttgtttatt tgcttagctt
     6121 tcgcttagcg acgtgttcac tttgcttgtt tgaattgaat tgtcgctccg tagacgaagc
     6181 gcctctattt atactccggc ggtcgagggt tcgaaatcga taagcttgga tcctaattga
     6241 attagctcta attgaattag tctctaattg aattagatcc cgtacgataa cttcgtatag
     6301 catacattat acgaagttat ctacgccccc aactgagaga actcaaaggt taccccagtt
     6361 ggggcactac gtcaaccggg gatcttttaa cataaacttc cagtagatgt acaagcagaa
     6421 aaaagagcca ttagcacggc agttaccatt gcttatgatt ccttgtgtcc aaaataatga
     6481 caaataggta tataaataat taaatgccaa acataagcga ttctaattta cctttacatc
     6541 tgtatgcatt tacatattat ccagaaaaca gacagcgata acttgcaaca ttgcttagta
     6601 taataatcca aagaaggaat ttaggcagaa attccagtta attaaatatt caaaacaaac
     6661 tttatttagt gcctcaataa tagtttggcc ctgctaattc tcctatttta ttttttaggg
     6721 attccggcca ctctgaccta tataaacatg gaccgcagtt tgacgggttc accggagcta
     6781 attccgtatc cagattggcg ctcaaataca gctggagatt gcgccaacag tattaccact
     6841 gcctaccgca ttaaagtgga tgagtgtggt cggctgtggg ttttggacac tggaaccgtg
     6901 ggcatcggca ataccaccac taatccgtgc ccctatgcgg taaatgtctt tgacttgacc
     6961 acggatacgc gaattcggag atacgagcta cctggcgtgg acacaaatcc aaatactttc
     7021 atagctaaca ttgccgtgga tataggcaaa aattgcgatg atgcatatgc ctattttgcc
     7081 gatgaattgg gatacggctt gattgcttac tcctgggaac tgaacaagtc ctggagattc
     7141 tcggcacatt cgtatttttt ccccgatcca ttgaggggcg atttcaatgt cgctggtatt
     7201 aacttccaat ggggcgagga gggtatattt ggtatgtccc tttcgcccat tcgatcggat
     7261 ggttatcgta ccctgtactt tagtccgtta gcaagtcatc gacaatttgc cgtatccacg
     7321 aggattttga gggatgaaac caggacggaa gatagctatc atgactttgt tgccttagat
     7381 gaacgggg
//
