p9344-BurrH vector (V015543)

LOCUS       62056_2235        9344 bp DNA     circular SYN 22-SEP-2020
DEFINITION  Cloning vector p9344-BurrH, complete sequence.
ACCESSION   MT081208
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Aksel T, Yu Z, Cheng Y, Douglas SM.
  TITLE     Molecular goniometers for single-particle cryo-EM of DNA-binding 
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   Aksel T, Yu Z, Cheng Y, Douglas SM.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 9344)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-FEB-2020) Cellular "
FEATURES             Location/Qualifiers
     source          1..9344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BuD binding
                     /note="BuD binding"
     misc_feature    34..38
                     /label=MCS
                     /note="MCS"
     primer_bind     complement(42..58)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             600..1829
                     /label=M13 gene II
                     /note="pII"
     CDS             1844..2104
                     /label=M13 gene V
                     /note="pV"
     CDS             2109..2207
                     /label=M13 gene VII
                     /note="pVII"
     CDS             2207..2302
                     /label=M13 gene IX
                     /note="pIX"
     CDS             2302..2520
                     /label=M13 gene VIII
                     /note="pVIII"
     terminator      2527..2574
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             2579..3850
                     /label=M13 gene III
                     /note="pIII"
     CDS             3856..4191
                     /label=M13 gene VI
                     /note="pVI"
     CDS             4196..5239
                     /label=M13 gene I
                     /note="pI"
     CDS             5220..6497
                     /label=M13 gene IV
                     /note="pIV"
     rep_origin      6488..6868
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     protein_bind    7107..7128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7143..7173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7181..7197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7205..7221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(7418..8006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8180..9037)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9038..9142)
                     /label=AmpR promoter