LOCUS       62056_2235        9344 bp DNA     circular SYN 22-SEP-2020
DEFINITION  Cloning vector p9344-BurrH, complete sequence.
ACCESSION   MT081208
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Aksel T, Yu Z, Cheng Y, Douglas SM.
  TITLE     Molecular goniometers for single-particle cryo-EM of DNA-binding 
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   Aksel T, Yu Z, Cheng Y, Douglas SM.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 9344)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-FEB-2020) Cellular "
FEATURES             Location/Qualifiers
     source          1..9344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BuD binding
                     /note="BuD binding"
     misc_feature    34..38
                     /label=MCS
                     /note="MCS"
     primer_bind     complement(42..58)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             600..1829
                     /label=M13 gene II
                     /note="pII"
     CDS             1844..2104
                     /label=M13 gene V
                     /note="pV"
     CDS             2109..2207
                     /label=M13 gene VII
                     /note="pVII"
     CDS             2207..2302
                     /label=M13 gene IX
                     /note="pIX"
     CDS             2302..2520
                     /label=M13 gene VIII
                     /note="pVIII"
     terminator      2527..2574
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             2579..3850
                     /label=M13 gene III
                     /note="pIII"
     CDS             3856..4191
                     /label=M13 gene VI
                     /note="pVI"
     CDS             4196..5239
                     /label=M13 gene I
                     /note="pI"
     CDS             5220..6497
                     /label=M13 gene IV
                     /note="pIV"
     rep_origin      6488..6868
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     protein_bind    7107..7128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7143..7173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7181..7197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7205..7221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(7418..8006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8180..9037)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9038..9142)
                     /label=AmpR promoter
ORIGIN
        1 ttaagagaag caaatacgtt aagcatgttg taaagcttgg cactggccgt cgttttacaa
       61 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      121 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      181 agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
      241 tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
      301 cacggttacg atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg
      361 tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
      421 tggctacagg aaggccagac gcgaattatt tttgatggcg ttcctattgg ttaaaaaatg
      481 agctgattta acaaaaattt aatgcgaatt ttaacaaaat attaacgttt acaatttaaa
      541 tatttgctta tacaatcttc ctgtttttgg ggcttttctg attatcaacc ggggtacata
      601 tgattgacat gctagtttta cgattaccgt tcatcgattc tcttgtttgc tccagactct
      661 caggcaatga cctgatagcc tttgtagatc tctcaaaaat agctaccctc tccggcatta
      721 atttatcagc tagaacggtt gaatatcata ttgatggtga tttgactgtc tccggccttt
      781 ctcacccttt tgaatcttta cctacacatt actcaggcat tgcatttaaa atatatgagg
      841 gttctaaaaa tttttatcct tgcgttgaaa taaaggcttc tcccgcaaaa gtattacagg
      901 gtcataatgt ttttggtaca accgatttag ctttatgctc tgaggcttta ttgcttaatt
      961 ttgctaattc tttgccttgc ctgtatgatt tattggatgt taatgctact actattagta
     1021 gaattgatgc caccttttca gctcgcgccc caaatgaaaa tatagctaaa caggttattg
     1081 accatttgcg aaatgtatct aatggtcaaa ctaaatctac tcgttcgcag aattgggaat
     1141 caactgttat atggaatgaa acttccagac accgtacttt agttgcatat ttaaaacatg
     1201 ttgagctaca gcattatatt cagcaattaa gctctaagcc atccgcaaaa atgacctctt
     1261 atcaaaagga gcaattaaag gtactctcta atcctgacct gttggagttt gcttccggtc
     1321 tggttcgctt tgaagctcga attaaaacgc gatatttgaa gtctttcggg cttcctctta
     1381 atctttttga tgcaatccgc tttgcttctg actataatag tcagggtaaa gacctgattt
     1441 ttgatttatg gtcattctcg ttttctgaac tgtttaaagc atttgagggg gattcaatga
     1501 atatttatga cgattccgca gtattggacg ctatccagtc taaacatttt actattaccc
     1561 cctctggcaa aacttctttt gcaaaagcct ctcgctattt tggtttttat cgtcgtctgg
     1621 taaacgaggg ttatgatagt gttgctctta ctatgcctcg taattccttt tggcgttatg
     1681 tatctgcatt agttgaatgt ggtattccta aatctcaact gatgaatctt tctacctgta
     1741 ataatgttgt tccgttagtt cgttttatta acgtagattt ttcttcccaa cgtcctgact
     1801 ggtataatga gccagttctt aaaatcgcat aaggtaattc acaatgatta aagttgaaat
     1861 taaaccatct caagcccaat ttactactcg ttctggtgtt tctcgtcagg gcaagcctta
     1921 ttcactgaat gagcagcttt gttacgttga tttgggtaat gaatatccgg ttcttgtcaa
     1981 gattactctt gatgaaggtc agccagccta tgcgcctggt ctgtacaccg ttcatctgtc
     2041 ctctttcaaa gttggtcagt tcggttccct tatgattgac cgtctgcgcc tcgttccggc
     2101 taagtaacat ggagcaggtc gcggatttcg acacaattta tcaggcgatg atacaaatct
     2161 ccgttgtact ttgtttcgcg cttggtataa tcgctggggg tcaaagatga gtgttttagt
     2221 gtattctttt gcctctttcg ttttaggttg gtgccttcgt agtggcatta cgtattttac
     2281 ccgtttaatg gaaacttcct catgaaaaag tctttagtcc tcaaagcctc tgtagccgtt
     2341 gctaccctcg ttccgatgct gtctttcgct gctgagggtg acgatcccgc aaaagcggcc
     2401 tttaactccc tgcaagcctc agcgaccgaa tatatcggtt atgcgtgggc gatggttgtt
     2461 gtcattgtcg gcgcaactat cggtatcaag ctgtttaaga aattcacctc gaaagcaagc
     2521 tgataaaccg atacaattaa aggctccttt tggagccttt tttttggaga ttttcaacgt
     2581 gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc tattctcact ccgctgaaac
     2641 tgttgaaagt tgtttagcaa aatcccatac agaaaattca tttactaacg tctggaaaga
     2701 cgacaaaact ttagatcgtt acgctaacta tgagggctgt ctgtggaatg ctacaggcgt
     2761 tgtagtttgt actggtgacg aaactcagtg ttacggtaca tgggttccta ttgggcttgc
     2821 tatccctgaa aatgagggtg gtggctctga gggtggcggt tctgagggtg gcggttctga
     2881 gggtggcggt actaaacctc ctgagtacgg tgatacacct attccgggct atacttatat
     2941 caaccctctc gacggcactt atccgcctgg tactgagcaa aaccccgcta atcctaatcc
     3001 ttctcttgag gagtctcagc ctcttaatac tttcatgttt cagaataata ggttccgaaa
     3061 taggcagggg gcattaactg tttatacggg cactgttact caaggcactg accccgttaa
     3121 aacttattac cagtacactc ctgtatcatc aaaagccatg tatgacgctt actggaacgg
     3181 taaattcaga gactgcgctt tccattctgg ctttaatgag gatttatttg tttgtgaata
     3241 tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat gctggcggcg gctctggtgg
     3301 tggttctggt ggcggctctg agggtggtgg ctctgagggt ggcggttctg agggtggcgg
     3361 ctctgaggga ggcggttccg gtggtggctc tggttccggt gattttgatt atgaaaagat
     3421 ggcaaacgct aataaggggg ctatgaccga aaatgccgat gaaaacgcgc tacagtctga
     3481 cgctaaaggc aaacttgatt ctgtcgctac tgattacggt gctgctatcg atggtttcat
     3541 tggtgacgtt tccggccttg ctaatggtaa tggtgctact ggtgattttg ctggctctaa
     3601 ttcccaaatg gctcaagtcg gtgacggtga taattcacct ttaatgaata atttccgtca
     3661 atatttacct tccctccctc aatcggttga atgtcgccct tttgtctttg gcgctggtaa
     3721 accatatgaa ttttctattg attgtgacaa aataaactta ttccgtggtg tctttgcgtt
     3781 tcttttatat gttgccacct ttatgtatgt attttctacg tttgctaaca tactgcgtaa
     3841 taaggagtct taatcatgcc agttcttttg ggtattccgt tattattgcg tttcctcggt
     3901 ttccttctgg taactttgtt cggctatctg cttacttttc ttaaaaaggg cttcggtaag
     3961 atagctattg ctatttcatt gtttcttgct cttattattg ggcttaactc aattcttgtg
     4021 ggttatctct ctgatattag cgctcaatta ccctctgact ttgttcaggg tgttcagtta
     4081 attctcccgt ctaatgcgct tccctgtttt tatgttattc tctctgtaaa ggctgctatt
     4141 ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg attgggataa ataatatggc
     4201 tgtttatttt gtaactggca aattaggctc tggaaagacg ctcgttagcg ttggtaagat
     4261 tcaggataaa attgtagctg ggtgcaaaat agcaactaat cttgatttaa ggcttcaaaa
     4321 cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt cttagaatac cggataagcc
     4381 ttctatatct gatttgcttg ctattgggcg cggtaatgat tcctacgatg aaaataaaaa
     4441 cggcttgctt gttctcgatg agtgcggtac ttggtttaat acccgttctt ggaatgataa
     4501 ggaaagacag ccgattattg attggtttct acatgctcgt aaattaggat gggatattat
     4561 ttttcttgtt caggacttat ctattgttga taaacaggcg cgttctgcat tagctgaaca
     4621 tgttgtttat tgtcgtcgtc tggacagaat tactttacct tttgtcggta ctttatattc
     4681 tcttattact ggctcgaaaa tgcctctgcc taaattacat gttggcgttg ttaaatatgg
     4741 cgattctcaa ttaagcccta ctgttgagcg ttggctttat actggtaaga atttgtataa
     4801 cgcatatgat actaaacagg ctttttctag taattatgat tccggtgttt attcttattt
     4861 aacgccttat ttatcacacg gtcggtattt caaaccatta aatttaggtc agaagatgaa
     4921 attaactaaa atatatttga aaaagttttc tcgcgttctt tgtcttgcga ttggatttgc
     4981 atcagcattt acatatagtt atataaccca acctaagccg gaggttaaaa aggtagtctc
     5041 tcagacctat gattttgata aattcactat tgactcttct cagcgtctta atctaagcta
     5101 tcgctatgtt ttcaaggatt ctaagggaaa attaattaat agcgacgatt tacagaagca
     5161 aggttattca ctcacatata ttgatttatg tactgtttcc attaaaaaag gtaattcaaa
     5221 tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt tgtttcatca tcttcttttg
     5281 ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt tgtaacttgg tattcaaagc
     5341 aatcaggcga atccgttatt gtttctcccg atgtaaaagg tactgttact gtatattcat
     5401 ctgacgttaa acctgaaaat ctacgcaatt tctttatttc tgttttacgt gcaaataatt
     5461 ttgatatggt aggttctaac ccttccatta ttcagaagta taatccaaac aatcaggatt
     5521 atattgatga attgccatca tctgataatc aggaatatga tgataattcc gctccttctg
     5581 gtggtttctt tgttccgcaa aatgataatg ttactcaaac ttttaaaatt aataacgttc
     5641 gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa gtctaatact tctaaatcct
     5701 caaatgtatt atctattgac ggctctaatc tattagttgt tagtgctcct aaagatattt
     5761 tagataacct tcctcaattc ctttcaactg ttgatttgcc aactgaccag atattgattg
     5821 agggtttgat atttgaggtt cagcaaggtg atgctttaga tttttcattt gctgctggct
     5881 ctcagcgtgg cactgttgca ggcggtgtta atactgaccg cctcacctct gttttatctt
     5941 ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt agggctatca gttcgcgcat
     6001 taaagactaa tagccattca aaaatattgt ctgtgccacg tattcttacg ctttcaggtc
     6061 agaagggttc tatctctgtt ggccagaatg tcccttttat tactggtcgt gtgactggtg
     6121 aatctgccaa tgtaaataat ccatttcaga cgattgagcg tcaaaatgta ggtatttcca
     6181 tgagcgtttt tcctgttgca atggctggcg gtaatattgt tctggatatt accagcaagg
     6241 ccgatagttt gagttcttct actcaggcaa gtgatgttat tactaatcaa agaagtattg
     6301 ctacaacggt taatttgcgt gatggacaga ctcttttact cggtggcctc actgattata
     6361 aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa aatcccttta atcggcctcc
     6421 tgtttagctc ccgctctgat tctaacgagg aaagcacgtt atacgtgctc gtcaaagcaa
     6481 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6541 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6601 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6661 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     6721 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6781 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     6841 gatttataag ggattttgcc gatttcggaa ccaccatcaa acaggatttt cgcctgctgg
     6901 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     6961 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     7021 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7081 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7141 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7201 cacacaggaa acagctatga ccatgattac gaattccttc cgcttcctcg ctcactgact
     7261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     9181 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     9241 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     9301 cttgtctgta agcggatgcc gggagcaggt cgactaatct atcg
//
