pSynBio3 vector (V015482)

LOCUS       62056_20300        4050 bp DNA     circular SYN 29-APR-2019
DEFINITION  Expression vector pSynBio3, complete sequence.
ACCESSION   MK047657
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Pogorevc D, Tang Y, Hoffmann MG, Zipf G, Bernauer HS, Popoff A, 
            Steinmetz H, Wenzel SC.
  TITLE     Biosynthesis and heterologous production of argyrins
  JOURNAL   ACS Synth Biol (2019) In press
  PUBMED    30995838
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Pogorevc D.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2018) Microbial Natural Products, Helmholtz 
            Institute for Pharmaceutical Research Saarland, Helmholtz Center for
            Infection Research, Saarland University Campus E8.1, Saarbruecken, 
            Saarland 66123, Germany
REFERENCE   3  (bases 1 to 4050)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-OCT-2018) Microbial Natural Products, Helmholtz Institute for 
            Pharmaceutical Research Saarland, Helmholtz Center for Infection 
            Research, Saarland University Campus E8.1, Saarbruecken, Saarland 
            66123, Germany"
FEATURES             Location/Qualifiers
     source          1..4050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      10..148
                     /regulatory_class="promoter"
     misc_feature    149..292
                     /label=MCS
                     /note="MCS"
     regulatory      525..573
                     /regulatory_class="terminator"
     rep_origin      626..1171
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             1767..2579
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        2676..2704
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2752..3939
                     /label=TcR
                     /note="tetracycline efflux protein"