LOCUS       62056_20300        4050 bp DNA     circular SYN 29-APR-2019
DEFINITION  Expression vector pSynBio3, complete sequence.
ACCESSION   MK047657
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Pogorevc D, Tang Y, Hoffmann MG, Zipf G, Bernauer HS, Popoff A, 
            Steinmetz H, Wenzel SC.
  TITLE     Biosynthesis and heterologous production of argyrins
  JOURNAL   ACS Synth Biol (2019) In press
  PUBMED    30995838
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Pogorevc D.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2018) Microbial Natural Products, Helmholtz 
            Institute for Pharmaceutical Research Saarland, Helmholtz Center for
            Infection Research, Saarland University Campus E8.1, Saarbruecken, 
            Saarland 66123, Germany
REFERENCE   3  (bases 1 to 4050)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-OCT-2018) Microbial Natural Products, Helmholtz Institute for 
            Pharmaceutical Research Saarland, Helmholtz Center for Infection 
            Research, Saarland University Campus E8.1, Saarbruecken, Saarland 
            66123, Germany"
FEATURES             Location/Qualifiers
     source          1..4050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      10..148
                     /regulatory_class="promoter"
     misc_feature    149..292
                     /label=MCS
                     /note="MCS"
     regulatory      525..573
                     /regulatory_class="terminator"
     rep_origin      626..1171
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             1767..2579
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        2676..2704
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2752..3939
                     /label=TcR
                     /note="tetracycline efflux protein"
ORIGIN
        1 tgaacatgtt ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt
       61 gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg
      121 ggatcaagat ccgatcaaga gacaggatca tatgccctga cctaggagta ctcctgcagg
      181 tgatcaacgc gtgagctcgc atgcgttaac ggtaccaggc ctggcgcgcc tctagagcgg
      241 ccgcacatgt caattgtggc caatgcatat taatccatgg ccctgaagat ctgattcctg
      301 gacgttgacc cgtgaccgtg agccgccctg gcgtcccggg tcgacttcca aggtcccacc
      361 cgggccggag acaggctgcg gctcgaggct gcgatgacga cgagcaactc cgggggaaac
      421 gggcgaagcc cgcgatggcg agggtcatgg gtggtagggc gctccggacc cggatactct
      481 tcctcgtggg acaggccggc ggggtgacct tcgaggtggt gcggccaccc tgcaagggcc
      541 cggaaccgca aggttccggg cccttcgcct tttgtttaaa ctgaccactt aagtgagcgt
      601 cagacccctt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct
      661 ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact
      721 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt
      781 agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct
      841 gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag
      901 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
      961 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
     1021 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag ggggaaacgc
     1081 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     1141 atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc
     1201 ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc
     1261 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc
     1321 ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc acatgaagca
     1381 cttcactgac accctcatca gtgccaacat agtaagccag tatacactcc gctagcgctg
     1441 aggtctgcct cgtgaagaag gtgttgctga ctcataccaa gcctgaatcg ccccatcatc
     1501 cagccagaaa gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt
     1561 gattttgaat atttgctttg ccacggaact agtctgcgtt gtcgggaaga tgcgtgatct
     1621 gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc
     1681 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     1741 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcga
     1801 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1861 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     1921 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     1981 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     2041 atgatgcgtg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
     2101 aatatcctga ttcaggtgaa aacattgttg atgcgctggc agtgttcctg cgccggttgc
     2161 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     2221 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     2281 gctggcctgt tgaacaagtc tggaaagaaa tgcacaagct tttgccattc tcaccggatt
     2341 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2401 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2461 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2521 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     2581 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     2641 ggatatctga cccttaatta acgtaattct catgtttgac agcttatcat cgattagctt
     2701 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct
     2761 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
     2821 gttatgccgg tactgccggg cctcttgcgg gacatcgtcc attccgacag catcgccagt
     2881 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
     2941 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
     3001 actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggattctcta cgccggacgc
     3061 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     3121 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     3181 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttaca tgcaccattc
     3241 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     3301 tcgcataagg gagagcgccg acccatgccc ttgagagcct tcaacccagt cagctccttc
     3361 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     3421 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     3481 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     3541 gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     3601 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     3661 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     3721 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     3781 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     3841 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     3901 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
     3961 tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga
     4021 atgcgcaaac caacccttga tttaaatccc
//
