pJL03 vector (V015469)

LOCUS       62056_13185        7994 bp DNA     circular SYN 02-NOV-2020
DEFINITION  Cloning vector pJL03, complete sequence.
ACCESSION   MT370812
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     A set of shuttle plasmids for gene expression in Acinetobacter 
            baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) First Hospital, Jilin University, Xi'an 
            Road, Changchun, Jilin 130062, China
REFERENCE   3  (bases 1 to 7994)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2020) First Hospital, Jilin University, Xi'an Road, 
            Changchun, Jilin 130062, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(530..1060)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1249..1277)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(1295..1399)
                     /label=AmpR promoter
     CDS             complement(1478..2353)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        2380..2664
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             2705..2728
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     rep_origin      complement(2970..3558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      4957..6292
                     /label=Acb oriV
                     /note="Acb oriV"
                     /regulatory_class="replication_regulatory_region"