LOCUS       62056_13185        7994 bp DNA     circular SYN 02-NOV-2020
DEFINITION  Cloning vector pJL03, complete sequence.
ACCESSION   MT370812
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     A set of shuttle plasmids for gene expression in Acinetobacter 
            baumannii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Jie J, Chu X, Li D, Luo Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2020) First Hospital, Jilin University, Xi'an 
            Road, Changchun, Jilin 130062, China
REFERENCE   3  (bases 1 to 7994)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-APR-2020) First Hospital, Jilin University, Xi'an Road, 
            Changchun, Jilin 130062, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(530..1060)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1249..1277)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(1295..1399)
                     /label=AmpR promoter
     CDS             complement(1478..2353)
                     /label=araC
                     /note="L-arabinose regulatory protein"
     promoter        2380..2664
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             2705..2728
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     rep_origin      complement(2970..3558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      4957..6292
                     /label=Acb oriV
                     /note="Acb oriV"
                     /regulatory_class="replication_regulatory_region"
ORIGIN
        1 cccctcatgt gcatggggga agtgatgaaa agaaacccat cacgcccaca tactaatgac
       61 aacaagaagc cttacaccgc aagggctagg gaagaaagtc agggagctag atcagaactc
      121 aacgctgaaa aaaatcaggg aaaaagtggc ggatctgacg aacgtccacc tagagcgagc
      181 agggcttgag gtgagagttg atcaccgcag tcataaggat cgtggactag acatcgagcc
      241 gacattcaag gagggacttg aagccaccca cgccaagcgc aggggggagg aactggagat
      301 cgtcaagcgt aatgatgaaa tcaaaaagcg caataaaaaa gctgaaaggc ttgatatgtc
      361 tatcattgag acgagcaaca gagtagagca attgacactt gaacagcagc aacagcagca
      421 ggggctgcat acttggtctg acaatcgatg cgaattggcc gcggcgttgt gacaatttac
      481 cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac
      541 ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca
      601 ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg
      661 cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg
      721 agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg ggcagaacgt
      781 aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct
      841 tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta
      901 cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag
      961 ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc
     1021 gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc
     1081 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca
     1141 aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg
     1201 gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc
     1261 gaacaggctt atgtcaattc ggttgaatac tcatactctt cctttttcaa tattattgaa
     1321 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1381 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc ctatgctact
     1441 ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat
     1501 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa
     1561 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag
     1621 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc
     1681 ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc
     1741 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt
     1801 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt
     1861 ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc
     1921 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg
     1981 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat
     2041 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat
     2101 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa
     2161 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat
     2221 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg
     2281 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt
     2341 gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt
     2401 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa
     2461 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta
     2521 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac
     2581 tttgctatgc catagcattt ttatccataa gattagcgga tcgtacctga cgctttttat
     2641 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcaggagg aattcaccat
     2701 gggcgattac aaggacgacg atgacaaggg atccgggccc gggagatcct ctagagtcga
     2761 cctgcaggca tgcaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
     2821 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
     2881 ggtggtccca cctgacccca tgccgaactc agaagtgaaa caggcctgca aaaggccagg
     2941 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3001 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3061 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3121 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3181 tatctctgtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3241 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3301 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     3361 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     3421 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     3481 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3541 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     3601 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     3661 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgt
     3721 gatcccccct ggaggatgcc gtagaccaag cccccgcagt ctatgccacc gcacaacagg
     3781 cactatacga ccaccaacaa caccgccaaa cgcttataga tgtgtatttg attgaagtaa
     3841 aaacggatga gaagcgagca gagcatagat tttacaaggc tttgcaagac aatatcgacc
     3901 gcttgcagct tgataaagtg atcaaagagg gacgagcagc tacagcactg ctaagggaga
     3961 tcggagagcc actgccgcca ccagtgaaag tgaaaacagg ttggtttagt gctgattatt
     4021 acggctttga ggacatgctc acacgttggg ataatgatca atctagcctt tccatttatc
     4081 acagagagat aaaagaggag caacaggaac agcaacgaaa acaacagcag atagaaagag
     4141 aaactaaaca aaaacaggaa gatgacaaaa agaaacggca ggactatgaa agatatttat
     4201 caaaatacgg ctatgtcaaa gatgctgagt atgaccatat cagcgaccat gttgcttcag
     4261 acatttccat gtttacacgt cttcaggcgc aggcatgggc aagtaacgat atgcaagagt
     4321 acataaggtg ttcaaagcaa aaatacgagc agatcagcga cagaatagcc tatgagcgtg
     4381 acttgaaaaa aatcgggcaa ttgagccgta tcctggataa agacagacag gcacttgctg
     4441 atattttacc gcagttgatg ccttactatg agcagatgca acaggctgtt aataaacgga
     4501 aaattttact tgagaatgag cgacagccat catttaaacc acgttatgac caagaacagc
     4561 ctaagccgaa aaaagacaat gacctaactt tttaatgtgt tacaatagca ttcaaatgta
     4621 acacattgga gtgaaaaaca tgacagaagc gacttttacc tttcgggtag accatgacct
     4681 aaagcaagaa ttttcaagcc ttgctaagac tgttgatcgg tcgggggcgc agctaatccg
     4741 tgacttcatg cgtgactttg ttaagaagca acaggaagcc gcagactatg acaaatggtt
     4801 caagcagcag gtacagatcg gcttaaatga agccaatgca ggcaagttga ttccacatga
     4861 ggacgtaaag gcagagtttg ccgctagacg tgctgcaaca ttggcgaaac tggcagcgaa
     4921 gaatgaagat tgagtggact gaaacagctc gccaagatcg tagaaatatc tatgattatc
     4981 ttgaagaacg caaccctata gcagctattg aaattgatga tttaattgaa gaaaagacag
     5041 atttacttgt tgataatcga ctgatggggc gcacaggcag acagaaagat actagggagt
     5101 tagtgataca tccgcattat gtggttgtat atgacatcac tgatataata cggatactca
     5161 gagtgctaca cacatcgcag gagtggtcat gacttactca tgtactttgg attatttagt
     5221 gttataaaat cctgatttat aaattttttt tgttaaaaaa gataaaagcc ccttgcaatt
     5281 gcttggggct ttaccgtaat ttatggggta cagatcttcg atactgacat atcggcaatc
     5341 gaaagcatta aggtttgacg accgctaatg atttcaccac aggggcttaa tgtacctgtc
     5401 ttaaattcta aggttttaac tcgctttgtc aagcatagac cccaaaaatt tagccaatgt
     5461 ctgtaactca atctgtccat gtgtgggtga tgaggtacag tgacgctagc acacatcgga
     5521 aaaacgctat tactagggga actgaacaga gtagcggacg caatgagtag tcatttaatt
     5581 ggcggttatg agcgtgttca ggcggtgcta tcaatcgtaa tcataacagt ggcagcttga
     5641 tacagtgatg tcatccctga tgcgaaagcg accgaccgac ggtacatcga atgggaatac
     5701 tttagggtga tttttaagaa tcgctctagg gtgagtattt cccattcagc tctgctccct
     5761 ccctctggta ctttaatcaa aagcactact aaacatatgt ttttaaataa aaaatattga
     5821 tatagagata atattagtaa gaataattaa acaattgaat atagataaat cattgttaaa
     5881 taaagattaa ttattaaaat gaatgtatac ttatatataa atcaatgatt taaaatattt
     5941 gataaagaaa acttttcaaa aaaaatataa ttgagattgt gtcatttcgg tcaattctta
     6001 atatgttcca cgcaagtttt agctatggtg ctaaacagaa atttgctgaa aaagaacttt
     6061 tcactgaact ggttaaaatg taagcagcct gagagccgcc aaaaatttta aaaacaaacc
     6121 gccttaatca tcttcaaaaa atacctctaa aacctcacca tttgcgtttt aagacccata
     6181 tttcatcctg cccttatgtt cccatgctga tagctataaa gtgtctgtaa tcgcttccta
     6241 tgacgttcta ggctgttgat aacttttgga acaacgcaaa atgttaaaat ccgatcattt
     6301 tttaacctag ttattttcgt tacagggtaa ctttggtagt attatttcaa tatttagttg
     6361 ttacaggata acaaaatata tgttacaggg taattaattt agcgttacag gataactata
     6421 gttacagggt aacgttaaat agttatcctg taatttaaaa atagttgcag gatagcataa
     6481 atttttacaa ataaatttta taaaaagtta tcctgtaact aaaaacaatg ttatcctgta
     6541 actatgatat actatatggg agttaaagac atgaaagacc cgaacgatca gaaaacacag
     6601 gacatgctaa aagagccgcc aaaaaccaat gccgaacgac aaaaagcata tcgtgaaaaa
     6661 cgcaaaagcc ttgatagtca acgtttggag gtgtttatag ataagggtgt gtcggatatg
     6721 cttgcggaca tggtgggcgc agcaggggag agccaaaaag ccattttgac cgcattgatt
     6781 gagaaagagt ataagcggct gtatgcagtg aaaaaatagc aagctaaaaa agtacattga
     6841 ttcaaaaagt aatgcagata aaaaagaaac cctcgattta tgagggtttt ttataactac
     6901 taatgcctat gaaaataagt gcttatcatt ttctctaatg tacccaataa aatcggggtt
     6961 ttgttccaca agtttcttga tgtcattatc atgtttcttc agtaagtaag ccccgatcag
     7021 gatcttgcgt tttgcatcgt ctgaccttgc ctgtttttgc tctcgacctt tcaccacagc
     7081 gtcagctaga tcctttcttg ccttttcctg acgttccaat ttttcctttt cttcatcgag
     7141 catttgaccc aattccatag ctgttttttt gaccattttt aatgcctgtg tctcaaccta
     7201 ttttccgaag tatgaaccat catcaatgtg aaaagtgttc tacttcaaaa actcgactta
     7261 catcaagatc cgagcgcagc gagcaaaatc aaaaaacaaa gtcaaagcct tattgctctt
     7321 gcttttgctt ttagcctcgc agagttcccg aagggcgcac ttacgcaaaa tttttgctac
     7381 gccaaatttt gcaagtacgg tcagggaaac cccgacaccc caaccgccca aaacttgggg
     7441 cggctgttaa taaacaggtg atgaaaatgg caatttacca ttgtgaaatg cagaacattt
     7501 cgaggtcaga tggtcgctca atcgtggcat gtgcagcata ccgagcaggc gaaaaattgt
     7561 actgtgatac gtacggaaaa gagcaggact acaccaaaaa aacaggcatt gaatacaccc
     7621 aaatttttgc cccaactggg gcaagtcctg acatgttaga tcgtcaaacc ctatggaatc
     7681 gggtagagca atccgaacta aaaaagaacg gtgacatcaa acaggaggca agattagcaa
     7741 aggaagtaga gatagcattg ccgcatgaac tggataagac acagcgtcag gcacttgtta
     7801 ccgagttgtg ccagtcctta gttaaagcct atggagtggc ggtggacgta gcgatccatg
     7861 cccctcatgt gcatggggga aggagaaaga aacctcacgc ccacataaga cacaggcgtc
     7921 aggcaacttg ttaaccgagt tgtgccagtc cttagttaaa gcctatggag tggcggtgga
     7981 cgtagcgatc catg
//
