pNRVL-LEU2 vector (V015454)

MSDS

Basic Vector Information

Vector Name:: pNRVL-LEU2

Antibiotic Resistance:: Kanamycin

Length:: 5554 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Nemeth T.

pNRVL-LEU2 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNRVL-LEU2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_18240        5554 bp DNA     circular SYN 03-MAR-2020
DEFINITION  Cloning vector pNRVL-LEU2, complete sequence.
ACCESSION   MN989860
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2020) Department of Microbiology, University of 
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (24-JAN-2020) Department of Microbiology, University of Szeged, 
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    570..575
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     regulatory      1096..1943
                     /label=Candida maltosa LEU2 promoter
                     /note="Candida maltosa LEU2 promoter"
                     /regulatory_class="terminator"
     CDS             1944..3062
                     /gene="LEU2"
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     regulatory      3066..3280
                     /label=Candida maltosa LEU2 terminator
                     /note="Candida maltosa LEU2 terminator"
                     /regulatory_class="terminator"
     misc_feature    3313..3318
                     /label=BssHII site for insert ligation
                     /note="BssHII site for insert ligation"
     misc_feature    3326..3331
                     /label=XhoI site for insert ligation
                     /note="XhoI site for insert ligation"
     misc_feature    3332..3782
                     /note="site of recombination; CpNEUT5LDowndown from 
                     C.parapsilosis"
     misc_feature    3783..3788
                     /label=StuI site for cassette release
                     /note="StuI site for cassette release"
     promoter        complement(3796..3814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3819..3835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3948..4754
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4904..5492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

This page is informational only.