LOCUS       V015454                 5554 bp    DNA     circular SYN 03-MAR-2020
DEFINITION  Exported.
ACCESSION   V015454
VERSION     V015454
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Nemeth T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2020) Department of Microbiology, University of
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (24-JAN-2020) Department of Microbiology, University of Szeged,
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
FEATURES             Location/Qualifiers
     source          1..5554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    570..575
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     regulatory      1096..1943
                     /label="Candida maltosa LEU2 promoter"
                     /note="Candida maltosa LEU2 promoter"
                     /regulatory_class="terminator"
     CDS             1944..3062
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     regulatory      3066..3280
                     /label="Candida maltosa LEU2 terminator"
                     /note="Candida maltosa LEU2 terminator"
                     /regulatory_class="terminator"
     misc_feature    3313..3318
                     /label="BssHII site for insert ligation"
                     /note="BssHII site for insert ligation"
     misc_feature    3326..3331
                     /label="XhoI site for insert ligation"
                     /note="XhoI site for insert ligation"
     misc_feature    3332..3782
                     /note="site of recombination; CpNEUT5LDowndown from
                     C.parapsilosis"
     misc_feature    3783..3788
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     promoter        complement(3796..3814)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3819..3835)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3948..4754
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4904..5492
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagtcaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacactagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccta ggccttgaat tggattggga ttagattagg
      601 atttgatggg tttttttttt tctgttaaat ggttatataa tcttcgtttc tgtgagatgt
      661 tttttttttt ctaaattaaa attgtattat gtaatggatt atgtaatttt gcattgcatt
      721 ttttagttat agtagtagta atagtagtac tgcatataca aacacactat tgtagatgag
      781 ggtagtgtac tattgcacaa atttggttta ctcctatttc gcatcattgc agttcatggt
      841 ttgtatgcag taatatatat ttatctctat ttctgtctct cctaagacca gtacaactta
      901 ccactcaatc acacgatata gtctacgagg ctacttcttt atctaccact ggcaatataa
      961 tggtaaagag agcttttccc aagaaccagg tgtggaataa tgaaaagtct atctatggaa
     1021 ggatacatgt tctagatctg cggccgcagc tcggatccac tagtaacggc cgccagtgtg
     1081 ctggaattcg cccttggatc caatcatcac tggtgggggt caagaaagag gtttgagatc
     1141 aggtactttg gcaccaccat tagtagctgg gtttggtgaa gctgcaagat taatgaaaca
     1201 agaatctgca tttgacaaga aacatattga gaggttatct actaaattga agaacggttt
     1261 gttatccatt ccatctactc agtttaacgg ttgtaataac ccaacatatc aatacccagg
     1321 ttgtgtgaat gtttcctttg catacattga aggggaatct ttgttaatgg cattgaaaga
     1381 tattgcattg agttctggtt ctgcatgtac ctctgcatct ttggaaccat cgtatgtttt
     1441 acatgctttg ggtgccgatg atgccttggc tcattcttcc attagattcg gtattggtag
     1501 attcaccact gaagcagaag ttgactatgt tattcaagca attaatgaaa gagttgattt
     1561 cttgagaaag atgtctccat tatgggaaat ggttcaagaa ggtattgact tgaactctat
     1621 cgaatggagt ggacattaag gttttttgtt tttttgtaat ttgtgttgtt atgtgtaatt
     1681 atcgtgtata gagtaatttc ggaaattgta ctttcgttca tgtatctgaa ccagatgaat
     1741 aatacgttcg tattgttgaa gaatgactaa ctcctgtaac ggcgtgcgtg ggttccgcag
     1801 ccgggttcaa tttttttttt cttgttcact ataaaaatat ataataaacg aaaaaaatcc
     1861 gagggactca ggctatgaaa tttttcactc aagaattttt tttccttcat ccttaattcc
     1921 tttattcact acccaaaccc acaatgtcag ttaaaactaa aactattaca attttaccag
     1981 gtgatcacgt cggtaccgaa attgtcaatg aagctatcaa agttttagaa gcaattgaag
     2041 ctgccactcc ttaccaaaaa atccatttcg atttcaaaca tcatttaatc ggtggtgctg
     2101 ccattgatgc cactggtgtc ccattgccag atgacgcact tgaaagtgcc aaaaaatctg
     2161 atgccgtctt attgggtgct gttggtggac caaaatgggg gactggcgcc gttcgtccag
     2221 aacaaggttt attaaagatt cgtaaagaat tgaaccttta tgctaacatc agaccatgta
     2281 actttgccag tgactcctta ttggaattat ccccattgag accagaagtc gttaaaggta
     2341 caaacttgat cattgttcgt gaattagttg gtggtattta tttcggtgat cgtgaagaac
     2401 aagaagaaag tgaagataaa caaaccgcat gggatactga aaaatacacc gtcgatgaag
     2461 tcaccagaat cacccgtatg gctgccttca tggctttaca acataaccca ccattaccaa
     2521 tctggtcatt ggataaggct aatgttttag cttcttctag attatggaga agaactgtcg
     2581 ataaagtgat ttctgaagag ttcccaactt tgtctgttca acatcaattg attgactccg
     2641 ccgccatgat tttaattcaa aacccaacca aattgaatgg tataatcatc acttctaaca
     2701 tgtttggtga tatcatttcc gatgaagctt cagttattcc aggttccttg ggtttattac
     2761 catctgcatc tttggcttca ttgccagata ccaacaccgc ttttggtctt tatgaaccat
     2821 gtcacggttc tgctcctgat ttaccagcca ataaagttaa cccaattgct actattttat
     2881 ctgctgcttc tatgttgaga ttatctttgg attgtgtgaa agaagctgaa gctttagaag
     2941 aagccgtgaa acaagtcttg gataaaggta tcagaactgc agatttaaga ggtaccagtt
     3001 cagctactga agttggtgac gcgattgtcg aagctgttac taaaatctta aaagaaaaag
     3061 cttaagtaaa gtatacacaa aatttaacga atattattaa tacaaaatga acaccaccta
     3121 aatacttttt tttaaaaatg taccgactct tctaaaaatt gcgataattc aatatcggaa
     3181 atttctctca ccggtttact tggatcttcg gaaataaatc tatttgaagt aatggtagcc
     3241 tcacttgatt ctgattggct ctttctagac atgggtaggt aagggcgaat tctgcagata
     3301 tccatcacac tggcgcgcta cggatctcga gcgaagtagt cctcactgag gagatcccag
     3361 gctagaaatc agcaaatttt acatccctca tattctgaca cgagtttaac tacatagcaa
     3421 ttgtaaaagg gctcttgttt tttttttttt tttgtggcgg tgcagtacgt acacgatctt
     3481 taaagattag ggctataacc atgtcaaagt gactcatttt ttttgttcaa atgcgcactc
     3541 ggatttaaag cagcaaaaaa atacttcgtt acaaaaatgg aagcgcattt tccgaaaaag
     3601 gttaattcct agcgatttta ttatttttgt tttatttatt aatttttctt gttttgctat
     3661 tgcatttttg caaccaaaaa tagaaaattc tcgttttttt ttgttgtagc gcaaccaatt
     3721 tctggttatt cttttttttt tgcaattttt aaatttgcac gtgacagtgt tagtgttagt
     3781 gcaggcctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     3841 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     3901 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     3961 aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     4021 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc
     4081 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     4141 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     4201 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat
     4261 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     4321 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     4381 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     4441 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc
     4501 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     4561 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     4621 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     4681 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     4741 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     4801 tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt
     4861 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4921 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4981 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5041 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5101 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5161 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5221 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5281 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5341 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5401 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5461 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5521 tcctggcctt ttgctggcct tttgctcaca tgtt
//
