AF-11-19 vector (V015444)

LOCUS       62056_281        9928 bp DNA     circular SYN 17-NOV-2020
DEFINITION  Cloning vector AF-11-19, complete sequence.
ACCESSION   MT926370
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Williams AE, Sanchez-Vargas I, Reid WR, Lin J, Franz AWE., Olson KE.
  TITLE     The Antiviral Small-Interfering RNA Pathway Induces Zika Virus 
            Resistance in Transgenic Aedes aegypti
  JOURNAL   Viruses 12 (11), E1231 (2020)
  PUBMED    33142991
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Williams A, Reid W, Sanchez-Vargas I, Lin J, Franz AWE., Kenneth OE.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2020) Veterinary Pathobiology, University of 
            Missouri, Connaway Hall, Columbia, MO 65211, USA
REFERENCE   3  (bases 1 to 9928)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Viruses"; 
            date: "2020"; volume: "12"; issue: "11"; pages: "E1231"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-AUG-2020) Veterinary Pathobiology, University of Missouri, 
            Connaway Hall, Columbia, MO 65211, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: plasmidSPAdes v. 3.12.0
            Assembly Name         :: AF-11-19
            Coverage              :: 2000
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    239..1476
                     /label=upstream homology arm
                     /note="upstream homology arm"
     regulatory      complement(1507..1748)
                     /label=simian virus 40 large T antigen terminator
                     /note="simian virus 40 large T antigen terminator"
                     /regulatory_class="terminator"
     misc_feature    1783..2320
                     /label=ZIKV dsRNA trigger
                     /note="ZIKV dsRNA trigger"
     misc_feature    complement(2321..2392)
                     /label=sialokinin-1 intron
                     /note="sialokinin-1 intron"
     misc_feature    complement(2393..2930)
                     /label=ZIKV dsRNA trigger
                     /note="ZIKV dsRNA trigger"
     regulatory      complement(2937..4093)
                     /label=carboxypeptidase A promoter
                     /note="carboxypeptidase A promoter"
                     /regulatory_class="promoter"
     protein_bind    4108..4207
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     polyA_signal    complement(4310..4431)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4557..5273)
                     /label=ECFP
                     /note="enhanced CFP"
     regulatory      complement(5274..5545)
                     /label=3xP3 eyeless promoter
                     /note="3xP3 eyeless promoter"
                     /regulatory_class="promoter"
     protein_bind    complement(5607..5706)
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     misc_feature    5723..7465
                     /label=downstream homology arm
                     /note="downstream homology arm"
     promoter        7970..8074
                     /label=AmpR promoter
     CDS             8075..8932
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      9106..9694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"