LOCUS       62056_281        9928 bp DNA     circular SYN 17-NOV-2020
DEFINITION  Cloning vector AF-11-19, complete sequence.
ACCESSION   MT926370
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Williams AE, Sanchez-Vargas I, Reid WR, Lin J, Franz AWE., Olson KE.
  TITLE     The Antiviral Small-Interfering RNA Pathway Induces Zika Virus 
            Resistance in Transgenic Aedes aegypti
  JOURNAL   Viruses 12 (11), E1231 (2020)
  PUBMED    33142991
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Williams A, Reid W, Sanchez-Vargas I, Lin J, Franz AWE., Kenneth OE.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2020) Veterinary Pathobiology, University of 
            Missouri, Connaway Hall, Columbia, MO 65211, USA
REFERENCE   3  (bases 1 to 9928)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Viruses"; 
            date: "2020"; volume: "12"; issue: "11"; pages: "E1231"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-AUG-2020) Veterinary Pathobiology, University of Missouri, 
            Connaway Hall, Columbia, MO 65211, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: plasmidSPAdes v. 3.12.0
            Assembly Name         :: AF-11-19
            Coverage              :: 2000
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     misc_feature    239..1476
                     /label=upstream homology arm
                     /note="upstream homology arm"
     regulatory      complement(1507..1748)
                     /label=simian virus 40 large T antigen terminator
                     /note="simian virus 40 large T antigen terminator"
                     /regulatory_class="terminator"
     misc_feature    1783..2320
                     /label=ZIKV dsRNA trigger
                     /note="ZIKV dsRNA trigger"
     misc_feature    complement(2321..2392)
                     /label=sialokinin-1 intron
                     /note="sialokinin-1 intron"
     misc_feature    complement(2393..2930)
                     /label=ZIKV dsRNA trigger
                     /note="ZIKV dsRNA trigger"
     regulatory      complement(2937..4093)
                     /label=carboxypeptidase A promoter
                     /note="carboxypeptidase A promoter"
                     /regulatory_class="promoter"
     protein_bind    4108..4207
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     polyA_signal    complement(4310..4431)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4557..5273)
                     /label=ECFP
                     /note="enhanced CFP"
     regulatory      complement(5274..5545)
                     /label=3xP3 eyeless promoter
                     /note="3xP3 eyeless promoter"
                     /regulatory_class="promoter"
     protein_bind    complement(5607..5706)
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     misc_feature    5723..7465
                     /label=downstream homology arm
                     /note="downstream homology arm"
     promoter        7970..8074
                     /label=AmpR promoter
     CDS             8075..8932
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      9106..9694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 taatcggtgt caattccatc aaccagtaaa acccatgctt tggaccatta ccatttaatc
      301 tcccaaaaat tagtatgcgt agttaagtac tacaatcaag cgggaaatgt catgcgtgcg
      361 ccgtacttcg ttcaattata catttttctt ctgttttgac atccatgtgc agcgtcgtgt
      421 gtatgggttg gaagtgataa taataataat aatgatcaca tgcagggaac tcacaggaat
      481 gcttatgcta tgctaggcag gcgtgccggg cgtaaatgta cttaacatta caatttatta
      541 tgtatatcat tattactgca tgttgttttg ttttttcttt catttttgca tttcaaatga
      601 tgatggcata cgcttcaata tatgatttcc tgagcatgtt cgaaccgatg tagagccaat
      661 ttattccatt aataataaac taggcatgtc cttccttacg ctccaagtta aagcattcat
      721 acgtttgcat aaacttaaca gcaaaacatt gcttaagctg gtatgctaag aaagaaaaaa
      781 aaacgattga acccaaaact agacagacaa aattattcca gtctgtggaa cttggtagtg
      841 agcagtagaa agaatcccgc cgcatatgca cacaaacagg aaatatattt tggcaaattg
      901 catgcgacag aagtgagtca cagatgttct tcggatagca ccaatactag cggttttgtg
      961 ttattcgata aaggcataac tgtcccacta agaagtacag tttattatga tagaaaatgc
     1021 aaagattacc acgaataaaa ataaccacca aatcacacac cagatattac tataagctag
     1081 agcattttgt cattgcatat acatactcga atatcggaca gtcatgcctt tatggaggaa
     1141 ctggtcacca aatcagattt cctgatgact agcggtaaaa aatgctaaac agcatcccgt
     1201 gcgaggctac cctagaaacc cattaacgag atgccttctc ctgattcctt cccggagaag
     1261 taagaacgaa taatcggaga agtcttgctt ttcgattgta ttgtgtaggt tggtagatgg
     1321 gagaaattag acctgaagaa tcaacattcg ctgatttgtt gaactgcgct acgctttgcc
     1381 gaaatgcatg cgatttattt tgatacctat tttttttcaa ataggtattg tgtaaactag
     1441 acgtaacaat aaaccgagcc atactgtcgc ctgagactcg accaccgcgg tggcggccgc
     1501 tctagctaag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1561 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1621 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1681 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctagagt
     1741 cgcggccgag atctccgcgg tttctctagt ggatcccccg ggcgaccaac aacaccataa
     1801 tggaagacag tgtgccggca gaggtgtgga ccagacacgg agagaaaaga gtgctcaaac
     1861 cgaggtggat ggacgccaga gtttgttcag atcatgcggc cctgaagtca ttcaaggagt
     1921 ttgccgctgg gaaaagagga gcggcttttg gagtgatgga agccctggga acactgccag
     1981 gacacatgac agagagattc caggaagcca ttgacaacct cgctgtgctc atgcgggcag
     2041 agactggaag caggccttac aaagccgcgg cggcccaatt gccggagacc ctagagacca
     2101 taatgctttt ggggttgctg ggaacagtct cgctgggaat cttcttcgtc ttgatgagga
     2161 acaagggcat agggaagatg ggctttggaa tggtgactct tggggccagc gcatggctca
     2221 tgtggctctc ggaaattgag ccagccagaa ttgcatgtgt cctcattgtt gtgttcctat
     2281 tgctggtggt gctcatacct gagccagaaa agcaaagatc agctgcctaa tgatatattt
     2341 ttaatcacta acagaacttt gaacaaaatc tgatgattgg gtgctcacct ccgatctttg
     2401 cttttctggc tcaggtatga gcaccaccag caataggaac acaacaatga ggacacatgc
     2461 aattctggct ggctcaattt ccgagagcca catgagccat gcgctggccc caagagtcac
     2521 cattccaaag cccatcttcc ctatgccctt gttcctcatc aagacgaaga agattcccag
     2581 cgagactgtt cccagcaacc ccaaaagcat tatggtctct agggtctccg gcaattgggc
     2641 cgccgcggct ttgtaaggcc tgcttccagt ctctgcccgc atgagcacag cgaggttgtc
     2701 aatggcttcc tggaatctct ctgtcatgtg tcctggcagt gttcccaggg cttccatcac
     2761 tccaaaagcc gctcctcttt tcccagcggc aaactccttg aatgacttca gggccgcatg
     2821 atctgaacaa actctggcgt ccatccacct cggtttgagc actcttttct ctccgtgtct
     2881 ggtccacacc tctgccggca cactgtcttc cattatggtg ttgttggtcg ctcgagcggc
     2941 cgcctgcagc ccgggctagt gattcgggat ccaactaacc gatacacact aacctggcca
     3001 attgcaatca ggtgccattt atagttcgct tgagcacatc gttaagtgga aatagttgtc
     3061 ttatcagcta cggtacctca ttggagctta ttccgcgatt taactcccgt tgggtacttg
     3121 aaagtagaag tctgcctttg atccatgctg acggattcat tcttagtttt atttaacgtt
     3181 tttcccactg attatgatag gaatcgcttt gacttttaaa ataagcgata ttcaaaacat
     3241 ggtggcctta aagaacagta gggtgaagtc ggataggttc acagcaggta ttctaattta
     3301 aacaatctac catggagaac agggcagaat ttacaaatgt ggaactctgg aaccacagta
     3361 agaagggctt ttttttagta atatttggtg atgttgtagt attagataaa atataactaa
     3421 ggtggctaag catcagataa aagaaacgta ttaactttct ggttttgaat tgaggtatta
     3481 aagttctcat tataaaacgt agttttaaac ttttttaaac acattttaaa tgcttctttg
     3541 gttcactagc tcaacaagac ttttaaaact caacccatta caattcaaaa acaaataaat
     3601 gcttaaacac tcttaaaata ttttgctttg aacgtatttt tttattgacg aattccatgt
     3661 cacatcggtc agtcacagat ctctccgttc tgtaaagtac agtcacccca cagttataga
     3721 tcaactaagg gtcatttttc agaacaaaat gtgattaaaa atcattagtg gcgttgtaca
     3781 aaacaaaatc acctcgcagg cagtgaaaaa tataggggaa tttacgtatt ctctaaacag
     3841 ctgaactttt aaaaaggcag ttatacacaa caataaatca caacaattag caggagacac
     3901 ctgaactaca cttgagcagt cttcatttat tggttattat acacaaaaca ctattttgtt
     3961 ctctaaatca tctatttttc cttacgaaag tcacgaggca cttgttcttt tatcggcaat
     4021 gcaattacta ttgtcggcaa ctaaaatgtt ctcttcggca atccaaaact gtgcaaaaac
     4081 tagcacgcgt aagaggcttc acgttttccc aggtcagaag cggttttcgg gagtagtgcc
     4141 ccaactgggg taacctttga gttctctcag ttgggggcgt agggccgccg acatgacaca
     4201 aggggttgtg accggggtgg acacgtacgc gggtgcttac gaccgtcagt cgcgcgagcg
     4261 cgactagtgc tagagtcgcg gtcgagccgt acgcgtattc gataagcttt aagatacatt
     4321 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
     4381 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
     4441 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
     4501 taaaacctct acaaatgtgg tatggctgat tatgatctag agtcgcggcc gctttacttg
     4561 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
     4621 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
     4681 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
     4741 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tggccttgat gccgttcttc
     4801 tgcttgtcgg cggtgatata gacgttgtgg ctgatgtagt tgtactccag cttgtgcccc
     4861 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
     4921 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
     4981 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     5041 tggtcggggt agcggctgaa gcactgcacg ccccaggtca gggtggtcac gagggtgggc
     5101 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
     5161 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     5221 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg
     5281 accggtggat cgtaccgtcg actctagcgg taccccgatt gtttagcttg ttcagctgcg
     5341 cttgtttatt tgcttagctt tcgcttagcg acgtgttcac tttgcttgtt tgaattgaat
     5401 tgtcgctccg tagacgaagc gcctctattt atactccggc ggtcgagggt tcgaaatcga
     5461 taagcttgga tcctaattga attagctcta attgaattag tctctaattg aattagatcc
     5521 ccgggcgagc tcgaatttct ctagcactag tcgcgctcgc gcgactgacg gtcgtaagca
     5581 cccgcgtacg tgtccacccc ggtcacaacc ccttgtgtca tgtcggcggc cctacgcccc
     5641 caactgagag aactcaaagg ttaccccagt tggggcacta ctcccgaaaa ccgcttctga
     5701 cctgggaaaa cgtgaagctc gactgcggtt ggtcgtgtat ttgaagcacg tagaaataca
     5761 atctagtctg tcgctccaac agagtacaat gaccgatagg ggagggaatt tgatgagata
     5821 taataatacg attttgtaat gcaattatca ttggatagag taatggcaca ggctatagag
     5881 ggttcaacga gtataattgt cactgatgaa ttttgcacct aattgaattt cggattataa
     5941 ttttgggtag atttcaatca aattgattaa tccctatgaa tattgagtaa aattttagta
     6001 atcttcttct tctatataaa taaaaatgga atggtgtttg tatgtcacga aatggcttac
     6061 gaacgggtca acggatttgc atgatccttt ctccgctttg ttcgtcaagg gttccaccgt
     6121 gtttgtgtgt atacaaatca caggatattc accaaaaaaa agttgaaaaa aaaagcgaaa
     6181 gtgaacggaa ctgttgtttt gtacgggaaa atccatggcg tttttcaaca gcctacttga
     6241 tggcaagacg aagtttgccg ggaccactag tttaagataa agttgaaacg aagccacaca
     6301 tggaatactc aagagaacaa tttcgatatt caaaaggtac aactttggca cctgtactta
     6361 aaagttgtgg ctggaacatt gcaacccgaa gttactgaac acataaaagc gtcgataact
     6421 tctttctact atattgctcg tatcttttga ctcaatacac caattctcca aattgtaacg
     6481 gttatgtaaa ggggatcagt tgcagtttca aatgcatcca cacggccggg aatgcaatgt
     6541 tcacctgcta ttccaccagt gattttcaaa tggtaaaaaa agtttctgat gaatcgtctc
     6601 aaaattctga tcaaaatcca ctatggattc tcattatatt cctctgagta ttctcattga
     6661 aatcttttct cctctcatca gaatcatctc tagattcttc ctagaattat ctcaggatta
     6721 tcctatgctg aatcttatca gaactctcgt gagattctta tcagcatcct gtcagaattc
     6781 ttatcagaat ctttccagga ttctcaacaa aatccttcag agttctcatc aaattttgtc
     6841 ctgaattccc tctcagattt ctcatgattc ttatgagaat cttctcggaa ttatcatcag
     6901 aatccttcct agattctcat cacaatcctt ccagatttct gatcagaatt gttttttttt
     6961 attaagacgc ggataccgtc ttcagccctt tagctgcacg gactgtaaat caacactaga
     7021 caacgggcaa gcatgctcca atggtacagc cgagaaacat tcctgacgaa aagtttcaac
     7081 gggctgtagc aggaatcgaa tccaaagccc atgacactat gctcttaaat gcctggcgac
     7141 actaaccgca tggccacgag gcccacaggt tctcatcata attcttccaa gagtctcgtc
     7201 aaaattcttt cagggttctc atcgaaatcc ttacaggttt ctcaagagaa gccaggaatt
     7261 tcattataat gcttcctcgt ttttcgtcag tcttccatga ctctcatgac ggctcttcca
     7321 ggattatcat cagaatagct cctgaattct cttaagagtc gttccaggat attcatcaca
     7381 atccttctat gtttctcatc tgaaacccta cagtattgtt gataaaaaaa aattctgaca
     7441 aaaatcctgt accagaaacc ttcccccgag ctcgaattca ctggccgtcg ttttacaacg
     7501 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     7561 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     7621 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     7681 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     7741 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     7801 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     7861 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     7921 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7981 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8041 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8101 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8161 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     8221 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     8281 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8341 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8401 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8461 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8521 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8581 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8641 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8701 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8761 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8821 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8881 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8941 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     9001 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     9061 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9121 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9181 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9241 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9301 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9361 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9421 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9481 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9541 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9601 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9661 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9721 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9781 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9841 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9901 ccccgcgcgt tggccgattc attaatgc
//
