pMD19-SMAX1D2 vector (V015433)

LOCUS       62056_16655        8378 bp DNA     circular SYN 05-MAY-2020
DEFINITION  Binary vector pMD19-SMAX1D2, complete sequence.
ACCESSION   MN508835
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Nelson D, Khosla A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2019) Botany and Plant Sciences, University of 
            California-Riverside, 5412 Boyce Hall, Riverside, CA 92507, USA
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-SEP-2019) Botany and Plant Sciences, University of 
            California-Riverside, 5412 Boyce Hall, Riverside, CA 92507, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(133..154)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(442..1030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1204..2061)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2062..2166)
                     /label=AmpR promoter
     primer_bind     2640..2656
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2693..4290
                     /label=UBQ10 promoter
                     /note="UBQ10 promoter"
                     /regulatory_class="promoter"
     CDS             4306..4326
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     protein_bind    4342..4366
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4369..5565
                     /label=D2
                     /note="D2"
     protein_bind    complement(5567..5591)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5599..6153
                     /label=Gaussia-Dura Luc
                     /note="enhanced variant of secreted Gaussia luciferase"
     CDS             6250..6315
                     /label=F2A
                     /note="2A peptide from foot-and-mouth disease virus
                     polyprotein"
     CDS             6322..7965
                     /label=Red Firefly Luciferase
                     /note="intracellular red firefly luciferase"
     terminator      7985..8237
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8271..8287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8295..8311)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8319..8349)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"