pMD19-SMAX1D2 vector (V015433)

MSDS

Basic Vector Information

Vector Name:: pMD19-SMAX1D2

Antibiotic Resistance:: Ampicillin

Length:: 8378 bp

Type:: Binary vector

Replication origin:: ori

Source/Author:: Nelson D, Khosla A.

pMD19-SMAX1D2 vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMD19-SMAX1D2 vector Sequence

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Download GeneBank File(.gb)

LOCUS       62056_16655        8378 bp DNA     circular SYN 05-MAY-2020
DEFINITION  Binary vector pMD19-SMAX1D2, complete sequence.
ACCESSION   MN508835
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Nelson D, Khosla A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2019) Botany and Plant Sciences, University of 
            California-Riverside, 5412 Boyce Hall, Riverside, CA 92507, USA
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-SEP-2019) Botany and Plant Sciences, University of 
            California-Riverside, 5412 Boyce Hall, Riverside, CA 92507, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(133..154)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(442..1030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1204..2061)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2062..2166)
                     /label=AmpR promoter
     primer_bind     2640..2656
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2693..4290
                     /label=UBQ10 promoter
                     /note="UBQ10 promoter"
                     /regulatory_class="promoter"
     CDS             4306..4326
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     protein_bind    4342..4366
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4369..5565
                     /label=D2
                     /note="D2"
     protein_bind    complement(5567..5591)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5599..6153
                     /label=Gaussia-Dura Luc
                     /note="enhanced variant of secreted Gaussia luciferase"
     CDS             6250..6315
                     /label=F2A
                     /note="2A peptide from foot-and-mouth disease virus
                     polyprotein"
     CDS             6322..7965
                     /label=Red Firefly Luciferase
                     /note="intracellular red firefly luciferase"
     terminator      7985..8237
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8271..8287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8295..8311)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8319..8349)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"

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