LOCUS       62056_16655        8378 bp DNA     circular SYN 05-MAY-2020
DEFINITION  Binary vector pMD19-SMAX1D2, complete sequence.
ACCESSION   MN508835
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Nelson D, Khosla A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2019) Botany and Plant Sciences, University of 
            California-Riverside, 5412 Boyce Hall, Riverside, CA 92507, USA
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (25-SEP-2019) Botany and Plant Sciences, University of 
            California-Riverside, 5412 Boyce Hall, Riverside, CA 92507, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(133..154)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(442..1030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1204..2061)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2062..2166)
                     /label=AmpR promoter
     primer_bind     2640..2656
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2693..4290
                     /label=UBQ10 promoter
                     /note="UBQ10 promoter"
                     /regulatory_class="promoter"
     CDS             4306..4326
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     protein_bind    4342..4366
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4369..5565
                     /label=D2
                     /note="D2"
     protein_bind    complement(5567..5591)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5599..6153
                     /label=Gaussia-Dura Luc
                     /note="enhanced variant of secreted Gaussia luciferase"
     CDS             6250..6315
                     /label=F2A
                     /note="2A peptide from foot-and-mouth disease virus
                     polyprotein"
     CDS             6322..7965
                     /label=Red Firefly Luciferase
                     /note="intracellular red firefly luciferase"
     terminator      7985..8237
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8271..8287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8295..8311)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8319..8349)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 atcgtcgaac ggcaggcgtg caaacttggc gtaatcatgg tcatagctgt ttcctgtgtg
       61 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      121 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      181 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      241 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      301 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      361 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      421 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      481 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      541 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      601 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      661 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      721 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      781 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      841 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      901 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      961 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1021 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1081 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1141 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1201 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1261 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1321 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1381 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1441 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1501 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1561 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1621 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1681 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1741 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1801 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     1861 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     1921 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     1981 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2041 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2101 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaatgggggt
     2161 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     2221 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     2281 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     2341 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     2401 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     2461 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     2521 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     2581 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     2641 taaaacgacg gccagtgaat tagaactcgg tacgcgcgga tcttccagag attgacgtcg
     2701 tatgatcgcg aagcacccac cctaagccac attttaatcc ttctgttggc catgccccat
     2761 caaagttgca cttaacccaa gattgtggtg gagcttccca tgtttctcgt ctgtcccgac
     2821 ggtgttgtgg ttggtgcttt ccttacattc tgagcctctt tccttctaat ccactcatct
     2881 gcatcttctt gtgtccttac taatacctca ttggttccaa attccctccc tttaagcacc
     2941 agctcgtttc tgttcttcca cagcctccca agtatccaag ggactaaagc ctccacattc
     3001 ttcagatcag gatattcttg tttaagatgt tgaactctat ggaggtttgt atgaactgat
     3061 gatctaggac cggataagtt cccttcttca tagcgaactt attcaaagaa tgttttgtgt
     3121 atcattcttg ttacattgtt attaatgaaa aaatattatt ggtcattgga ctgaacacga
     3181 gtgttaaata tggaccaggc cccaaataag atccattgat atatgaatta aataacaaga
     3241 ataaatcgag tcaccaaacc acttgccttt tttaacgaga cttgttcacc aacttgatac
     3301 aaaagtcatt atcctatgca aatcaataat catacaaaaa tatccaataa cactaaaaaa
     3361 ttaaaagaaa tggataattt cacaatatgt tatacgataa agaagttact tttccaagaa
     3421 attcactgat tttataagcc cacttgcatt agataaatgg caaaaaaaaa caaaaaggaa
     3481 aagaaataaa gcacgaagaa ttccagaaaa tacgaaatac gcttcaatgc agtgggaccc
     3541 acggttcaat tattgccaat tttcagctcc accgtatatt taaaaaataa aacgataatg
     3601 ctaaaaaaat ataaatcgta acgatcgtta aatctcaacg gctggatctt atgacgaccg
     3661 ttagaaattg tggttgtcga cgagtcagta ataaacggcg tcaaagtggt tgcagccggc
     3721 acacacgagt cgtgtttatc aactcaaagc acaaatactt ttcctcaacc taaaaataag
     3781 gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca atacacgtgt cattttatta
     3841 ttagctattg cttcaccgcc ttagctttct cgtgacctag tcgtcctcgt cttttcttct
     3901 tcttcttcta taaaacaata cccaaagagt tcttcttctt cacaattcag atttcaattt
     3961 ctcaaaatct taaaaacttt ctctcaattc tctctaccgt gatcaaggta aatttctgtg
     4021 ttccttattc tctcaaaatc ttcgattttg ttttcgttcg atcccaattt cgtatatgtt
     4081 ctttggttta gattctgtta atcttagatc gaagacgatt ttctgggttt gatcgttaga
     4141 tatcatctta attctcgatt agggtttcat agatatcatc cgatttgttc aaataatttg
     4201 agttttgtcg aataattact cttcgatttg tgatttctat ccagatctgg tgttagtttc
     4261 tagtttgtgc gatcgaattt gtcctcgaga ggggactcta gaatgccaaa aaagaagaga
     4321 aaggtagaag accccactag tacaagtttg tacaaaaaag caggcttcat gagtgttttg
     4381 caaaaggaga atcttgggaa ttcgttagac attgatttgt ttaagaagct gttgaaggga
     4441 atgaccgaga aagtttggtg gcagaatgat gcagccgctg cggtggctgc aactgttagt
     4501 cagtgcaagc tcgggaatgg gaaacgacgc ggtgttttat caaagggaga tgtctggtta
     4561 cttttttcag gaccggatag agtggggaag agaaaaatgg tgtcagctct gtcttctctt
     4621 gtgtatggga cgaatccaat aatgattcag ctcgggtcga gacaagatgc tggagatgga
     4681 aattctagtt tccgtggtaa aaccgcgttg gataagattg cggaaactgt taagaggagt
     4741 ccgttctctg ttatcttgct tgaagatatt gacgaagctg atatgttggt gcgtggaagc
     4801 ataaaacagg caatggatag aggcagaatc cgtgactcgc atggccgtga gatcagttta
     4861 ggtaacgtga tctttgttat gacagcgagc tggcatttcg cagggacgaa aacatcgttc
     4921 ctagacaatg aagcaaaact acgagatttg gctagcgaaa gctggcgttt gaggttgtgc
     4981 atgcgggaga aatttgggaa acgacgagca agttggctgt gtagcgacga ggagaggctg
     5041 acgaaaccga agaaagaaca tggatcgggt ttatctttcg atctgaacca agccgctgat
     5101 acggatgatg gttctcataa tacgagcgat ctaacaacag ataatgatca agacgagcaa
     5161 ggttttagtg gaaagctgtc actacaatgc gttccatttg cattccacga tatggttagc
     5221 cgtgtagatg atgcggtagc gtttcgagca gttgatttcg cagctgtgag acggagaatc
     5281 acagaaacat tatctgagag attcgaaacg atcataggcg aatcactatc agtagaagtc
     5341 gaggaagagg cacttcagag aatcttgagt ggagtgtggt taggccaaac agagttagag
     5401 gaatggattg agaaagcgat tgttccggtg ttgagccaac ttaaggctcg agtttcgtct
     5461 tctggtactt acggtgactg tacggttgct cggctcgagc tagatgaaga ttccggtgaa
     5521 cgaaacgccg gtgatttact gccgacaact attactttgg cagtataccc agctttcttg
     5581 tacaaagtgg tgggcccaat gggtgtcaaa gtgctattcg ctttgatctg catcgccgtt
     5641 gcagaagcaa aacccaccga aaataatgag gactttaaca tagtcgcggt agcgtctaac
     5701 tttgccacta cggatttgga cgctgacaga ggcaagctgc cgggtaaaaa acttccgctg
     5761 gaagttctga aagagcttga ggctaatgca cgtaaggcag gctgcaccag agggtgccta
     5821 atttgcttat cacacataaa gtgtacaccc aagatgaaga agtttattcc cggaagatgc
     5881 catacatacg aaggtgacaa agagtccgcc caaggaggta taggcgaagc catcgttgac
     5941 ataccagaga tcccaggatt caaggactta gagcctttag agcaattcat tgctcaggta
     6001 gatctctgcg tagactgcac aactgggtgc ctaaagggct tggcaaacgt ccaatgctca
     6061 gacttattga agaaatggtt accgcagaga tgtgcaacct ttgcctcaaa aatacagggt
     6121 caggtagaca aaataaaggg agcaggtggt gacacgcgtt ctaatgccgc cgatgaggtt
     6181 gcgactggat caggtctttt agcgatacat ccgaccgaag cgcgacataa acaaaaaatt
     6241 gtagcgccgg tcaaacaaac cctgaatttc gacttactca aacttgcggg tgacgtagag
     6301 tcaaatccag ggcctggtac catggagaat atggagaacg acgagaatat tgttgtcggg
     6361 cctaaacctt tttacccgat agaagagggg tctgccggga ctcagttaag aaaatacatg
     6421 gaacgttatg ccaaacttgg tgccatcgcg ttcacgaatg ctgtgaccgg ggtagattac
     6481 tcttacgccg aatatcttga aaaaagctgc tgtctaggga aggccctaca aaactacggg
     6541 cttgttgtcg acgggaggat cgcactatgt tctgaaaatt gcgaagagtt ctttatccca
     6601 gtaatcgcgg ggctatttat tggcgtcggg gtggcgccca ccaacgaaat atacacacta
     6661 cgagagcttg tccacagcct agggatctct aaacccacta tcgtattctc atctaagaag
     6721 ggactcgaca aggtaattac agtgcaaaag accgttacga ccatcaaaac aattgtcata
     6781 ctcgacagta aagtagacta caggggctat cagtgcctcg acacgtttat taagcgaaac
     6841 accccaccag gttttcaggc ttccagcttc aagactgtgg aagttgatcg aaaggaacaa
     6901 gtggcgctca tcatgaactc cagcggctca acgggtctcc ccaagggcgt tcaacttact
     6961 cacgaaaaca cggtaacccg ttttagccat gcacgagacc cgatctacgg caaccaggta
     7021 agccctggca ccgctgtctt aacagtggtt cctttccatc atgggtttgg aatgttcacc
     7081 acgctaggat atctaatttg cggctttaga gttgtgatgt tgacaaagtt tgatgaggaa
     7141 acttttctaa aaactctaca ggactacaaa tgcacatatg tgattttggt tccaacgctt
     7201 ttcgccattc ttaataaatc agaactgttg aacaagtacg atctcagtaa cttggtggag
     7261 atcgcttctg ggggtgcccc cctgtctaag gaagtaggag aggcagtggc tcgtcgtttt
     7321 aacttaccgg gggtccgtca aggatacggt ttgacggaaa cgacatccgc catcatcatt
     7381 acgccagagg gcgacgacaa acctggagct agtggtaaag ttgtgccttt atttaaggcg
     7441 aaggtaattg acctagacac gaagaagagt ctaggtccta ataggagagg ggaggtatgc
     7501 gtcaagggac ccatgctgat gaaaggttat gttaataacc cggaagcgac taaggaattg
     7561 attgacgagg aaggttggtt gcatacggga gatataggtt attatgacga ggaaaaacac
     7621 tttttcatag tagacaggct gaagtcattg attaaataca aagggtatca agtcccaccc
     7681 gccgagcttg aatctgttct tcttcaacat cctagcattt ttgacgctgg ggttgcggga
     7741 gttcccgatc cggttgcggg agagctgcca ggtgcagtag ttgtgttaga gtccgggaag
     7801 aatatgactg aaaaggaagt gatggactat gtggcaagtc aagtctccaa cgccaagaga
     7861 ctgagaggtg gcgtccgatt cgttgacgaa gttcccaagg ggctaacagg caagattgac
     7921 gggcgtgcaa tcagagagat attaaagaaa cccgtggcca aaatgtaaga gctcgaattt
     7981 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     8041 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     8101 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     8161 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     8221 atctatgtta ctagatcggg aattggttcc ggaaccaatt cgtaatcatg gtcatagctg
     8281 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8341 aagtgtaaag cctggggtgc ctaatgagtg agctaact
//
