pMJA295 vector (V015423)

LOCUS       V015423                 7557 bp    DNA     circular SYN 12-JUN-2019
DEFINITION  Exported.
ACCESSION   V015423
VERSION     V015423
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Wang R, Ghumra A, Fairclough L, Alcocer M.
  TITLE     Nut lipids are involved in the intrinsic allergenicity of nut
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Wang R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2019) School of Biosciences, University of
            Nottingham, Babs Building, Sutton Bonington LE12 5RD, UK
REFERENCE   3  (bases 1 to 7557)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-APR-2019) School of Biosciences, University of Nottingham, Babs
            Building, Sutton Bonington LE12 5RD, UK"
FEATURES             Location/Qualifiers
     source          1..7557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(570..625)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             1363..1374
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(1636..1839)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1840..2143)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             3071..3529
                     /gene="TRDC"
                     /label="T cell receptor delta constant"
                     /note="T cell receptor delta constant from Homo sapiens.
                     Accession#: B7Z8K6"
     polyA_signal    3570..3794
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3840..4268
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4282..4612
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4660..4707
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4726..5097
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5230..5363
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5400..5416)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5424..5440)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5448..5478)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5493..5514)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5802..6390)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6564..7421)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7422..7526)
                     /label="AmpR promoter"