Basic Vector Information
- Vector Name:
- pMJA295
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7557 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- Wang R, Ghumra A, Fairclough L, Alcocer M.
- Promoter:
- CMV
pMJA295 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMJA295 vector Sequence
LOCUS 62056_16995 7557 bp DNA circular SYN 12-JUN-2019 DEFINITION Mammalian expression vector pMJA295, complete sequence. ACCESSION MK764036 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7557) AUTHORS Wang R, Ghumra A, Fairclough L, Alcocer M. TITLE Nut lipids are involved in the intrinsic allergenicity of nut proteins JOURNAL Unpublished REFERENCE 2 (bases 1 to 7557) AUTHORS Wang R. TITLE Direct Submission JOURNAL Submitted (05-APR-2019) School of Biosciences, University of Nottingham, Babs Building, Sutton Bonington LE12 5RD, UK REFERENCE 3 (bases 1 to 7557) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (05-APR-2019) School of Biosciences, University of Nottingham, Babs Building, Sutton Bonington LE12 5RD, UK" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..7557 /mol_type="other DNA" /organism="synthetic DNA construct" polyA_signal complement(570..625) /label=beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" CDS 1363..1374 /label=Factor Xa site /note="Factor Xa recognition and cleavage site" promoter complement(1636..1839) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" enhancer complement(1840..2143) /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" CDS 3071..3529 /gene="TRDC" /label=TRDC /note="T cell receptor delta constant from Homo sapiens. Accession#: B7Z8K6" polyA_signal 3570..3794 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3840..4268 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4282..4612 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 4660..4707 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 4726..5097 /label=BleoR /note="antibiotic-binding protein" polyA_signal 5230..5363 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5400..5416) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(5424..5440) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5448..5478) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(5493..5514) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5802..6390) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6564..7421) /label=AmpR /note="beta-lactamase" promoter complement(7422..7526) /label=AmpR promoter
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