LOCUS       V015423                 7557 bp    DNA     circular SYN 12-JUN-2019
DEFINITION  Exported.
ACCESSION   V015423
VERSION     V015423
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Wang R, Ghumra A, Fairclough L, Alcocer M.
  TITLE     Nut lipids are involved in the intrinsic allergenicity of nut
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Wang R.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2019) School of Biosciences, University of
            Nottingham, Babs Building, Sutton Bonington LE12 5RD, UK
REFERENCE   3  (bases 1 to 7557)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-APR-2019) School of Biosciences, University of Nottingham, Babs
            Building, Sutton Bonington LE12 5RD, UK"
FEATURES             Location/Qualifiers
     source          1..7557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(570..625)
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     CDS             1363..1374
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     promoter        complement(1636..1839)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1840..2143)
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             3071..3529
                     /gene="TRDC"
                     /label="T cell receptor delta constant"
                     /note="T cell receptor delta constant from Homo sapiens.
                     Accession#: B7Z8K6"
     polyA_signal    3570..3794
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3840..4268
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4282..4612
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4660..4707
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4726..5097
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    5230..5363
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5400..5416)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5424..5440)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5448..5478)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5493..5514)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5802..6390)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6564..7421)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7422..7526)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagtaagaga agagggacag ctatgactgg gagtagtcag gagaggagga
      301 aaaatctggc tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca
      361 caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat
      421 ggacagcagg gggctgtttc atatactgat gacctcttta tagccacctt tgttcatggc
      481 agccagcata tggcatatgt tgccaaactc taaaccaaat actcattctg atgttttaaa
      541 tgatttgccc tcccatatgt ccttccgagt gagagacaca aaaaattcca acacactatt
      601 gcaatgaaaa taaatttcct ttattagcca gaagtcagat gctcaagggg cttcatgatg
      661 tccccataat ttttggcaga gggaaaaaga tctcagtggt atttgtgagc cagggcattg
      721 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttgaattcct
      781 taataaaccc aatgcctttt tcttattaat gcccaacttt gtgacccaag aaaggttggg
      841 gttagatttt cttttccatt tgcaggaaga aaacccgttt cttttaaggc agacagcagg
      901 tggatatggc caaaataggc ccccctcttg aggagccgga ggaggtacgt gtaatatgca
      961 gaggtgtttg tgagctgcag cattagtgta tcatttgcat cttttgacca attgtctttg
     1021 gggtccattg tgatgacatc atttgcatct tttgaataac tgtctttggg gtccactgtg
     1081 gtgacatctg tctttattgg aggaaagata atttcttgat caattccgtt tttattattc
     1141 tcatgtctga cgatacatct gtgttctttg tccagtgact cttctggcac cgttaaccag
     1201 ctaaatttca tgtatgtgtc gttagtcttc atggtgttcc cctcctggga gcccagaatc
     1261 gtgttgctct tcttttcttg ccaatgtatc ttaataatat ctgggaaaaa tttctcaaga
     1321 agacaaaggt atgttccagc cttctggagt tttgtttcag caattgaagg aagaaaaata
     1381 gtgggcttgg gggaaacatc tgcatcaagt tgtttatctg tgacaacaag tgttgttcca
     1441 ctgccgagac gaaagagttt cttataatac gcgtctcaca tgccttcctc tctgaggcct
     1501 tggaaggaaa gggaccagat gaagaggagc agaagatcct cttaaaactc aggccccact
     1561 caacaaggaa gtgatcccta gcacagaaga cagtaggagg gaggatccct agcggatctg
     1621 acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     1681 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     1741 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     1801 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     1861 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     1921 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     1981 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     2041 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     2101 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     2161 gctatgaact aatgaccccg taattgatta ctattaataa ctactcgagt atgtcgaggt
     2221 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2281 ggaggtacca ccggtcatat gccgcgggag cccagatcct gtgtgtgtgt gtgtgtgctt
     2341 gtgcacatct gttctctgat tggctgactg tacttcctga gagtccctgt ttaatgttac
     2401 aagaaacagc tgatgctgtt ctccagcctg ctgtgtgtat ttgtggcctt cagctactct
     2461 ggtatggatg gttccaggtg tgagtaaaaa tgttaaggat ggaaggattc caaacaggca
     2521 tatggtattt ctcatggact tgaggaggaa aggagaaatt gtaaaaaagc gagcatatta
     2581 tgattttagt ttggtttgtc tctgggtcct aaattagtct agctcttacg atattttatt
     2641 cactatatca aaggatcagc tctgttttct gatttttccc acaggatcaa gtgtggccca
     2701 gaaggttact caagcccagt catcagtatc catgccagtg aggaaagcag tcaccctgaa
     2761 ctgcctgtat gaaacaagtt ggtggtcata ttatattttt tggtacaagc agcctcctag
     2821 caaagagatg attttcctta ttcgccaggg ttctgatgaa cagaatgcaa aaagtggtcg
     2881 ctattctgtc aacttcaaga aagcagcgaa atccgtcgcc ttaaccattt cagccttaca
     2941 gctagaagat tcagcaaagt acttttgtgc tcttggggtc tatattccac ccctaaactg
     3001 ggggatacgc ctcacgtaca ccgataaact catctttgga aaaggaaccc gtgtgactgt
     3061 ggaaccaaga agtcagcctc ataccaaacc atccgttttt gtcatgaaaa atggaacaaa
     3121 tgtcgcttgt ctggtgaagg aattttaccc caaggatata agaataaatc tcgtgtcatc
     3181 caagaagata acagagtttg atcctgctat tgtcatctct cccggtggga agtacaatgc
     3241 tgtcaagctt ggtaaatatg aagattcaaa ttcagtgaca tgttcagttc aacacgacaa
     3301 taaaactgtg cactccactg actttgaagt gaagacagat tctacagatc acgtaaaacc
     3361 aaaggaaact gaaaacacaa agcaaccttc aaagagctgc cataaaccca aagccatagt
     3421 tcataccgag aaggtgaaca tgatgtccct cacagtgctt gggctacgaa tgctgtttgc
     3481 aaagactgtt gccgtcaatt ttctcttgac tgccaagtta tttttcttgt aatctagagg
     3541 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     3601 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     3661 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     3721 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     3781 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     3841 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3901 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3961 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag
     4021 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4081 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4141 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4201 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4261 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4321 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4381 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4441 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4501 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4561 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4621 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     4681 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     4741 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     4801 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     4861 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     4921 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     4981 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     5041 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     5101 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5161 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5221 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5281 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5341 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     5401 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     5461 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     5521 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5581 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5641 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5701 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5761 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5821 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5881 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5941 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     6001 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     6061 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     6121 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     6181 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     6241 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     6301 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     6361 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     6421 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     6481 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     6541 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     6601 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     6661 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     6721 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     6781 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     6841 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6901 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6961 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7021 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7081 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7141 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7201 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7261 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     7321 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     7381 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     7441 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     7501 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
