3XFlag-SMXL6-no-EAR vector (V015413)

MSDS

Basic Vector Information

Vector Name:: 3XFlag-SMXL6-no-EAR

Antibiotic Resistance:: Ampicillin

Length:: 7289 bp

Type:: Cloning vector

Replication origin:: ori

Host:: Plants

Source/Author:: Wang L.

Promoter:: CaMV 35S

3XFlag-SMXL6-no-EAR vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

3XFlag-SMXL6-no-EAR vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       62056_126        7289 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector 3XFlag-SMXL6-no-EAR, complete sequence.
ACCESSION   MN780594
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             448..513
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     protein_bind    514..538
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             541..3462
                     /codon_start=1
                     /transl_table=11
                     /product="AtSMXL6 no EAR"
                     /label=AtSMXL6 no EAR
                     /note="AtSMXL6 protein with EAR notif deletion"
                     /protein_id="QHH26575.1"
                     /translation="MPTPVTTARECLTEEAARALDDAVVVARRRSHAQTTSLHAVSALL
                     AMPSSILREVCVSRAARSVPYSSRLQFRALELCVGVSLDRLPSSKSPATEEDPPVSNSL
                     MAAIKRSQANQRRHPESYHLQQIHASNNGGGGCQTTVLKVELKYFILSILDDPIVNRVF
                     GEAGFRSSEIKLDVLHPPVTQLSSRFSRGRCPPLFLCNLPNSDPNREFPFSGSSGFDEN
                     SRRIGEVLGRKDKKNPLLIGNCANEALKTFTDSINSGKLGFLQMDISGLSLISIEKEIS
                     EILADGSKNEEEIRMKVDDLGRTVEQSGSKSGIVLNLGELKVLTSEANAALEILVSKLS
                     DLLKHESKQLSFIGCVSSNETYTKLIDRFPTIEKDWDLHVLPITASTKPSTQGVYPKSS
                     LMGSFVPFGGFFSSTSNFRVPLSSTVNQTLSRCHLCNEKYLQEVAAVLKAGSSLSLADK
                     CSEKLAPWLRAIETKEDKGITGSSKALDDANTSASQTAALQKKWDNICQSIHHTPAFPK
                     LGFQSVSPQFPVQTEKSVRTPTSYLETPKLLNPPISKPKPMEDLTASVTNRTVSLPLSC
                     VTTDFGLGVIYASKNQESKTTREKPMLVTLNSSLEHTYQKDFKSLREILSRKVAWQTEA
                     VNAISQIICGCKTDSTRRNQASGIWLALLGPDKVGKKKVAMTLSEVFFGGKVNYICVDF
                     GAEHCSLDDKFRGKTVVDYVTGELSRKPHSVVLLENVEKAEFPDQMRLSEAVSTGKIRD
                     LHGRVISMKNVIVVVTSGIAKDNATDHVIKPVKFPEEQVLSARSWKLQIKLGDATKFGV
                     NKRKYELETAQRAVKVQRSYVNETEFSPDHEAEDRDAWFDEFIEKVDGKVTFKPVDFDE
                     LAKNIQEKIGSHFERCFGSETHLELDKEVILQILAASWSSLSSGEEEGRTIVDQWMQTV
                     LARSFAEAKQKYGSNPMLGVKLVASSSGLASGVELPAKVDVIW"
     protein_bind    complement(3464..3488)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3753..3771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3778..3794)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3935..4390
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4468..4572
                     /label=AmpR promoter
     CDS             4573..5430
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5604..6192
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6480..6501
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6516..6546
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6554..6570
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6578..6594
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6612..6630
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        6803..7289
                     /label=CaMV35S(long)
                     /note="Cauliflower mosaic virus 35S promoter (long)"

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