LOCUS       62056_126        7289 bp DNA     circular SYN 20-JAN-2020
DEFINITION  Cloning vector 3XFlag-SMXL6-no-EAR, complete sequence.
ACCESSION   MN780594
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2019) State Key Laboratory of Plant Genomics, 
            Institute of Genetics and Developmental Biology, CAS, West Beichen 
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-NOV-2019) State Key Laboratory of Plant Genomics, Institute of 
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             448..513
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     protein_bind    514..538
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             541..3462
                     /codon_start=1
                     /transl_table=11
                     /product="AtSMXL6 no EAR"
                     /label=AtSMXL6 no EAR
                     /note="AtSMXL6 protein with EAR notif deletion"
                     /protein_id="QHH26575.1"
                     /translation="MPTPVTTARECLTEEAARALDDAVVVARRRSHAQTTSLHAVSALL
                     AMPSSILREVCVSRAARSVPYSSRLQFRALELCVGVSLDRLPSSKSPATEEDPPVSNSL
                     MAAIKRSQANQRRHPESYHLQQIHASNNGGGGCQTTVLKVELKYFILSILDDPIVNRVF
                     GEAGFRSSEIKLDVLHPPVTQLSSRFSRGRCPPLFLCNLPNSDPNREFPFSGSSGFDEN
                     SRRIGEVLGRKDKKNPLLIGNCANEALKTFTDSINSGKLGFLQMDISGLSLISIEKEIS
                     EILADGSKNEEEIRMKVDDLGRTVEQSGSKSGIVLNLGELKVLTSEANAALEILVSKLS
                     DLLKHESKQLSFIGCVSSNETYTKLIDRFPTIEKDWDLHVLPITASTKPSTQGVYPKSS
                     LMGSFVPFGGFFSSTSNFRVPLSSTVNQTLSRCHLCNEKYLQEVAAVLKAGSSLSLADK
                     CSEKLAPWLRAIETKEDKGITGSSKALDDANTSASQTAALQKKWDNICQSIHHTPAFPK
                     LGFQSVSPQFPVQTEKSVRTPTSYLETPKLLNPPISKPKPMEDLTASVTNRTVSLPLSC
                     VTTDFGLGVIYASKNQESKTTREKPMLVTLNSSLEHTYQKDFKSLREILSRKVAWQTEA
                     VNAISQIICGCKTDSTRRNQASGIWLALLGPDKVGKKKVAMTLSEVFFGGKVNYICVDF
                     GAEHCSLDDKFRGKTVVDYVTGELSRKPHSVVLLENVEKAEFPDQMRLSEAVSTGKIRD
                     LHGRVISMKNVIVVVTSGIAKDNATDHVIKPVKFPEEQVLSARSWKLQIKLGDATKFGV
                     NKRKYELETAQRAVKVQRSYVNETEFSPDHEAEDRDAWFDEFIEKVDGKVTFKPVDFDE
                     LAKNIQEKIGSHFERCFGSETHLELDKEVILQILAASWSSLSSGEEEGRTIVDQWMQTV
                     LARSFAEAKQKYGSNPMLGVKLVASSSGLASGVELPAKVDVIW"
     protein_bind    complement(3464..3488)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3753..3771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3778..3794)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3935..4390
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4468..4572
                     /label=AmpR promoter
     CDS             4573..5430
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5604..6192
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6480..6501
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6516..6546
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6554..6570
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6578..6594
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6612..6630
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        6803..7289
                     /label=CaMV35S(long)
                     /note="Cauliflower mosaic virus 35S promoter (long)"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcatggac tacaaagacc atgacggtga ttataaagat
      481 catgacatcg actacaagga tgacgatgac aagacaagtt tgtacaaaaa agcaggctta
      541 atgccgacgc cggtgactac ggcgagagaa tgcttgacgg aagaagctgc tcgtgccctc
      601 gacgatgctg ttgttgtagc tcgtcggaga agccacgcgc agacgacgtc tcttcatgca
      661 gtttctgctc ttttagctat gccgtcgtcg attctccgtg aagtttgcgt ctcacgcgcc
      721 gctaggagtg ttccttactc gtcgcgactt caattccgag ctcttgagct ctgcgtcggt
      781 gtatctctcg acaggcttcc gtcgtcgaag tctccggcga cggaagaaga tccaccggtt
      841 tcgaattcgc tcatggcggc gatcaaacgg tctcaggcga accagagacg gcatccggag
      901 tcgtatcatc ttcagcagat ccacgctagt aacaacggcg ggggaggatg ccagacgacg
      961 gttttgaaag tcgaattgaa gtatttcata ctctcgatcc ttgacgatcc gattgtgaat
     1021 cgggtattcg gagaagctgg gtttcggagc tccgaaatta agctcgatgt gcttcaccct
     1081 ccggtaacac aactttcttc ccgtttctct agaggtcgtt gtccgcctct cttcctctgt
     1141 aatcttccca actcagatcc gaatcgtgag ttcccgttta gtgggagcag tggtttcgat
     1201 gaaaattccc ggaggattgg agaagtatta ggcaggaaag ataagaagaa ccctctgctt
     1261 attggtaact gtgctaatga agctcttaaa acgttcacgg attcgatcaa cagtgggaag
     1321 ttagggtttc ttcagatgga tattagcgga ttaagcttga ttagtatcga gaaggagatt
     1381 agtgagattt tagccgatgg atcgaaaaac gaagaagaga ttcgaatgaa agtggatgac
     1441 ctaggaagaa ctgtagagca aagtggctcg aaatcgggga tagtgcttaa tctgggagag
     1501 ctcaaggttt tgaccagtga agccaatgcg gctcttgaga ttttggtgtc gaagctttcg
     1561 gatttgctga aacatgaaag taaacaactt tcgttcatcg gatgtgtatc gagtaatgag
     1621 acttacacga agcttattga tcggtttcct actatagaga aggattggga ccttcatgtt
     1681 cttccaatca cagcctctac taaaccttcg actcaagggg tttatcccaa atcgagcttg
     1741 atgggatcct ttgttccctt tggagggttc ttctcatcaa catcaaattt cagagttcca
     1801 ttgagtagca cagtgaatca gacgctctct agatgccacc tctgcaacga gaagtatttg
     1861 caagaagtag ccgctgttct caaggccggt tcgagtcttt ctctggctga caaatgttcc
     1921 gagaagttag ccccatggct acgggctatt gagaccaaag aagacaaggg aataacaggc
     1981 agcagtaagg ctttagatga cgctaataca tcagcctcgc aaaccgctgc tctacagaag
     2041 aaatgggaca acatatgcca aagtatccat cacactccgg cgtttcctaa acttggtttt
     2101 cagtcggtga gtccgcagtt cccagttcag actgagaaga gtgtgagaac tcctacaagc
     2161 tatttggaga cgcctaaact gctgaatccg ccaatctcaa agccaaaacc tatggaggat
     2221 cttacggcat cggtgactaa ccgcacagtg agtttgcctt tgagctgtgt tactacagat
     2281 tttgggttgg gagtaatcta tgcatccaaa aaccaggaat caaaaacaac gagggagaaa
     2341 ccgatgctgg tgactctaaa ctcttcttta gaacatacat atcagaaaga tttcaagtct
     2401 ctcagagaaa tactctctcg taaagttgcc tggcagaccg aagctgtaaa tgccataagc
     2461 caaattatct gcggatgcaa aaccgactcc acgcgaagaa accaagcaag cggaatttgg
     2521 ctggctcttc ttggacccga taaagtgggg aagaagaaag tggcgatgac tctttctgaa
     2581 gtcttctttg gtggtaaagt caattacata tgtgtagatt ttggggcaga gcattgttcc
     2641 cttgatgaca aattcagagg caaaacagtg gtggattacg taaccggtga gttatctagg
     2701 aaaccacact ctgttgtttt actcgaaaac gtggaaaaag ctgagttccc ggatcagatg
     2761 agattgtctg aagctgtgag tacggggaaa atccgtgatt tgcatggaag agtgattagt
     2821 atgaaaaatg tgattgttgt tgtgacgtct gggattgcca aggataatgc cactgaccat
     2881 gttattaaac ctgtgaagtt tcctgaggag caagttctca gcgcgagaag ctggaaactg
     2941 cagataaagc taggagatgc tactaaattt ggggtaaata agagaaaata tgagctagaa
     3001 acagcgcaac gtgcagtgaa ggtgcaacgt tcatatgtga atgaaacaga atttagccct
     3061 gatcatgagg cagaggacag ggacgcttgg ttcgatgaat tcattgaaaa agtagatgga
     3121 aaagtgacgt tcaaaccggt tgatttcgat gagttagcca agaacattca agagaagatt
     3181 ggttcacatt ttgagcggtg ctttggatcc gaaacacatc tagaacttga taaagaagtg
     3241 atccttcaga ttctggcggc ttcatggtca tcattatcat cgggcgaaga agaagggaga
     3301 acaatagttg atcagtggat gcaaacagtt cttgctcgaa gctttgctga agcaaaacag
     3361 aagtacggtt cgaatcccat gttgggcgtg aagctggttg cttcttctag cggcttagct
     3421 tccggagtag aattgccggc gaaggtggat gtgatatggt gatacccagc tttcttgtac
     3481 aaagtggtga tatcccgcgg ccatgctaga gtccgcaaaa atcaccagtc tctctctaca
     3541 aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat
     3601 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     3661 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga
     3721 cctgcaggca tgcgacgtcg ggcccaattc gccctatagt gagtcgtatt acaattcact
     3781 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3841 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3901 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     3961 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4021 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4081 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4141 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4201 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4261 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4321 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4381 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4441 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4501 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4561 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4621 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4681 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4741 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4801 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4861 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4921 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4981 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5041 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5101 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5161 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5221 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5281 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5341 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5401 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5461 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5521 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5761 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5821 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5881 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5941 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6001 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6061 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6121 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6181 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6241 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6301 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6361 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6421 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6481 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6541 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6601 tacgccaagc tatttaggtg acactataga atactcaagc tatgcatcca acgcgttggg
     6661 agctctccca tatggtcgac tagagccaag ctgatctcct ttgccccgga gatcaccatg
     6721 gacgactttc tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc
     6781 atgttcacca ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca
     6841 cagatggtta gagaggccta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat
     6901 ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact
     6961 aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta
     7021 tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag
     7081 aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca
     7141 gaactcgccg tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag
     7201 aagaaaatct tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
     7261 gatacagtct cagaagacca aagggctat
//
