Basic Vector Information
- Vector Name:
- pNRVL-LEU2-4kbp
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6355 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Nemeth TM.
pNRVL-LEU2-4kbp vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNRVL-LEU2-4kbp vector Sequence
LOCUS 62056_18225 6355 bp DNA circular SYN 20-MAR-2021
DEFINITION Cloning vector pNRVL-LEU2-4kbp, complete sequence.
ACCESSION MT948176
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6355)
AUTHORS Nemeth TM.
TITLE Direct Submission
JOURNAL Submitted (01-SEP-2020) Department of Microbiology, University of
Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE 2 (bases 1 to 6355)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(01-SEP-2020) Department of Microbiology, University of Szeged,
Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
COMMENT ##Assembly-Data-START##
Assembly Method :: CLC Genomics Workbench v. v11
Sequencing Technology :: IonTorrent
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..6355
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator complement(268..295)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(387..473)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 537..553
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 570..575
/label=StuI site for cassette release
/note="StuI site for cassette release"
misc_feature 576..1039
/note="Site of recombination; CpNEUT5LUpup from C.
parapsilosis"
misc_feature 1040..1047
/label=NotI site for selectable marker ligation
/note="NotI site for selectable marker ligation"
regulatory 1096..1943
/label=Candida maltosa LEU2 promoter
/note="Candida maltosa LEU2 promoter"
/regulatory_class="promoter"
CDS 1944..3062
/gene="LEU2"
/label=LEU2
/note="3-isopropylmalate dehydrogenase from Candida
maltosa. Accession#: P07139"
regulatory 3066..3280
/label=Candida maltosa LEU2 terminator
/note="Candida maltosa LEU2 terminator"
/regulatory_class="terminator"
misc_feature 3314..3319
/note="BssHII site for selectable marker and insert
ligation"
misc_feature 3320..4113
/note="Candida albicans intergenic region;
Ca22chrRB_C_albicans_SC5314:2080135-2080928"
misc_feature 4114..4119
/label=BssHII site for insert ligation
/note="BssHII site for insert ligation"
misc_feature 4127..4132
/label=XhoI site for insert ligation
/note="XhoI site for insert ligation"
misc_feature 4133..4583
/note="Site of recombination; CpNEUT5LDowndown from C.
parapsilosis"
misc_feature 4584..4589
/label=StuI site for cassette release
/note="StuI site for cassette release"
promoter complement(4597..4615)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4620..4636)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 4749..5555
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 5705..6293
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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