LOCUS       V015411                 6355 bp    DNA     circular SYN 20-MAR-2021
DEFINITION  Exported.
ACCESSION   V015411
VERSION     V015411
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Nemeth TM.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2020) Department of Microbiology, University of
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (01-SEP-2020) Department of Microbiology, University of Szeged,
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
FEATURES             Location/Qualifiers
     source          1..6355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    570..575
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     misc_feature    576..1039
                     /note="Site of recombination; CpNEUT5LUpup from C.
                     parapsilosis"
     misc_feature    1040..1047
                     /label="NotI site for selectable marker ligation"
                     /note="NotI site for selectable marker ligation"
     regulatory      1096..1943
                     /label="Candida maltosa LEU2 promoter"
                     /note="Candida maltosa LEU2 promoter"
                     /regulatory_class="promoter"
     CDS             1944..3062
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     regulatory      3066..3280
                     /label="Candida maltosa LEU2 terminator"
                     /note="Candida maltosa LEU2 terminator"
                     /regulatory_class="terminator"
     misc_feature    3314..3319
                     /note="BssHII site for selectable marker and insert
                     ligation"
     misc_feature    3320..4113
                     /note="Candida albicans intergenic region;
                     Ca22chrRB_C_albicans_SC5314:2080135-2080928"
     misc_feature    4114..4119
                     /label="BssHII site for insert ligation"
                     /note="BssHII site for insert ligation"
     misc_feature    4127..4132
                     /label="XhoI site for insert ligation"
                     /note="XhoI site for insert ligation"
     misc_feature    4133..4583
                     /note="Site of recombination; CpNEUT5LDowndown from C.
                     parapsilosis"
     misc_feature    4584..4589
                     /label="StuI site for cassette release"
                     /note="StuI site for cassette release"
     promoter        complement(4597..4615)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4620..4636)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             4749..5555
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5705..6293
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagtcaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacactagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccta ggccttgaat tggattggga ttagattagg
      601 atttgatggg tttttttttt tctgttaaat ggttatataa tcttcgtttc tgtgagatgt
      661 tttttttttt ctaaattaaa attgtattat gtaatggatt atgtaatttt gcattgcatt
      721 ttttagttat agtagtagta atagtagtac tgcatataca aacacactat tgtagatgag
      781 ggtagtgtac tattgcacaa atttggttta ctcctatttc gcatcattgc agttcatggt
      841 ttgtatgcag taatatatat ttatctctat ttctgtctct cctaagacca gtacaactta
      901 ccactcaatc acacgatata gtctacgagg ctacttcttt atctaccact ggcaatataa
      961 tggtaaagag agcttttccc aagaaccagg tgtggaataa tgaaaagtct atctatggaa
     1021 ggatacatgt tctagatctg cggccgcagc tcggatccac tagtaacggc cgccagtgtg
     1081 ctggaattcg cccttggatc caatcatcac tggtgggggt caagaaagag gtttgagatc
     1141 aggtactttg gcaccaccat tagtagctgg gtttggtgaa gctgcaagat taatgaaaca
     1201 agaatctgca tttgacaaga aacatattga gaggttatct actaaattga agaacggttt
     1261 gttatccatt ccatctactc agtttaacgg ttgtaataac ccaacatatc aatacccagg
     1321 ttgtgtgaat gtttcctttg catacattga aggggaatct ttgttaatgg cattgaaaga
     1381 tattgcattg agttctggtt ctgcatgtac ctctgcatct ttggaaccat cgtatgtttt
     1441 acatgctttg ggtgccgatg atgccttggc tcattcttcc attagattcg gtattggtag
     1501 attcaccact gaagcagaag ttgactatgt tattcaagca attaatgaaa gagttgattt
     1561 cttgagaaag atgtctccat tatgggaaat ggttcaagaa ggtattgact tgaactctat
     1621 cgaatggagt ggacattaag gttttttgtt tttttgtaat ttgtgttgtt atgtgtaatt
     1681 atcgtgtata gagtaatttc ggaaattgta ctttcgttca tgtatctgaa ccagatgaat
     1741 aatacgttcg tattgttgaa gaatgactaa ctcctgtaac ggcgtgcgtg ggttccgcag
     1801 ccgggttcaa tttttttttt cttgttcact ataaaaatat ataataaacg aaaaaaatcc
     1861 gagggactca ggctatgaaa tttttcactc aagaattttt tttccttcat ccttaattcc
     1921 tttattcact acccaaaccc acaatgtcag ttaaaactaa aactattaca attttaccag
     1981 gtgatcacgt cggtaccgaa attgtcaatg aagctatcaa agttttagaa gcaattgaag
     2041 ctgccactcc ttaccaaaaa atccatttcg atttcaaaca tcatttaatc ggtggtgctg
     2101 ccattgatgc cactggtgtc ccattgccag atgacgcact tgaaagtgcc aaaaaatctg
     2161 atgccgtctt attgggtgct gttggtggac caaaatgggg gactggcgcc gttcgtccag
     2221 aacaaggttt attaaagatt cgtaaagaat tgaaccttta tgctaacatc agaccatgta
     2281 actttgccag tgactcctta ttggaattat ccccattgag accagaagtc gttaaaggta
     2341 caaacttgat cattgttcgt gaattagttg gtggtattta tttcggtgat cgtgaagaac
     2401 aagaagaaag tgaagataaa caaaccgcat gggatactga aaaatacacc gtcgatgaag
     2461 tcaccagaat cacccgtatg gctgccttca tggctttaca acataaccca ccattaccaa
     2521 tctggtcatt ggataaggct aatgttttag cttcttctag attatggaga agaactgtcg
     2581 ataaagtgat ttctgaagag ttcccaactt tgtctgttca acatcaattg attgactccg
     2641 ccgccatgat tttaattcaa aacccaacca aattgaatgg tataatcatc acttctaaca
     2701 tgtttggtga tatcatttcc gatgaagctt cagttattcc aggttccttg ggtttattac
     2761 catctgcatc tttggcttca ttgccagata ccaacaccgc ttttggtctt tatgaaccat
     2821 gtcacggttc tgctcctgat ttaccagcca ataaagttaa cccaattgct actattttat
     2881 ctgctgcttc tatgttgaga ttatctttgg attgtgtgaa agaagctgaa gctttagaag
     2941 aagccgtgaa acaagtcttg gataaaggta tcagaactgc agatttaaga ggtaccagtt
     3001 cagctactga agttggtgac gcgattgtcg aagctgttac taaaatctta aaagaaaaag
     3061 cttaagtaaa gtatacacaa aatttaacga atattattaa tacaaaatga acaccaccta
     3121 aatacttttt tttaaaaatg taccgactct tctaaaaatt gcgataattc aatatcggaa
     3181 atttctctca ccggtttact tggatcttcg gaaataaatc tatttgaagt aatggtagcc
     3241 tcacttgatt ctgattggct ctttctagac atgggtaggt aagggcgaat tctgcagata
     3301 tccatcacac tgggcgcgct gcaactttgt tctaaatggt tcgattggat tctcccaatt
     3361 acacaaaaat tattattttc gtgctgtttt ggcgccgact tctatttttt aaatcccaac
     3421 agggattccg atccaaatca gcaggtattt ctatctgatt tcttttcaat ttggttgctt
     3481 ttttctgaac ttatttcgta ttaatgggca acaatttata aagattattc agaatactag
     3541 ttgaaaaaat tgtgtatagt aagcttcata tggaattaaa ttgaatgatt ttagttatgt
     3601 atttttaagg agtttctttc catactatgg gatatggaaa tatgtatatt ctatttcata
     3661 tcccttttct tgtttccaat ttgaatattc aatttccgaa aaacaaaaaa gaacacaaaa
     3721 caaaacaact aataatgatg atgttttcat tctgttttgt gtaatattcc ttctcctgac
     3781 taataaattg atatatatgg cataatacaa aaatgagtga agctaaattc taattgcatg
     3841 ttgcaattta ttctagcctt taaaaatgtc ggaattgaga atgtgttgta atgttgtatt
     3901 gtatccctta aactactaca ccattacagt ttttttttgt gttattgttt ttgaaaaaag
     3961 tcaaaattag aaagttacag ggatagtttc aatagtttct ggtaggttcc tcctaaagtt
     4021 gaatttgggt agaaagacag ccaggatcct tggattgttt agtatgaaca atttgtatta
     4081 ttattctcat actactacct cccgaaagtt tacgcgcgct acggatctcg agcgaagtag
     4141 tcctcactga ggagatccca ggctagaaat cagcaaattt tacatccctc atattctgac
     4201 acgagtttaa ctacatagca attgtaaaag ggctcttgtt tttttttttt ttttgtggcg
     4261 gtgcagtacg tacacgatct ttaaagatta gggctataac catgtcaaag tgactcattt
     4321 tttttgttca aatgcgcact cggatttaaa gcagcaaaaa aatacttcgt tacaaaaatg
     4381 gaagcgcatt ttccgaaaaa ggttaattcc tagcgatttt attatttttg ttttatttat
     4441 taatttttct tgttttgcta ttgcattttt gcaaccaaaa atagaaaatt ctcgtttttt
     4501 tttgttgtag cgcaaccaat ttctggttat tctttttttt ttgcaatttt taaatttgca
     4561 cgtgacagtg ttagtgttag tgcaggcctg atatccccta tagtgagtcg tattacatgg
     4621 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     4681 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     4741 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     4801 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     4861 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     4921 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     4981 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     5041 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     5101 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     5161 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     5221 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     5281 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     5341 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     5401 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     5461 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     5521 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     5581 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     5641 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5701 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5761 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5821 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5881 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5941 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6001 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6061 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6241 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6301 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
