3XFlag-SMXL6 vector (V015409)

LOCUS       V015409                 7307 bp    DNA     circular SYN 20-JAN-2020
DEFINITION  Exported.
ACCESSION   V015409
VERSION     V015409
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2019) State Key Laboratory of Plant Genomics,
            Institute of Genetics and Developmental Biology, CAS, West Beichen
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (22-NOV-2019) State Key Laboratory of Plant Genomics, Institute of
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             448..513
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     protein_bind    514..538
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             541..3477
                     /gene="SMXL6"
                     /label="Protein SMAX1-LIKE 6"
                     /note="Protein SMAX1-LIKE 6 from Arabidopsis thaliana.
                     Accession#: Q9LML2"
     protein_bind    complement(3482..3506)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3771..3789)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3796..3812)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3953..4408
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4486..4590
                     /label="AmpR promoter"
     CDS             4591..5448
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5622..6210
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6498..6519
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6534..6564
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6572..6588
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6596..6612
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6630..6648
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        6821..7307
                     /label="CaMV35S(long)"
                     /note="Cauliflower mosaic virus 35S promoter (long)"