LOCUS       V015409                 7307 bp    DNA     circular SYN 20-JAN-2020
DEFINITION  Exported.
ACCESSION   V015409
VERSION     V015409
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Wang L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2019) State Key Laboratory of Plant Genomics,
            Institute of Genetics and Developmental Biology, CAS, West Beichen
            Road, Bei Jing 100101, China
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (22-NOV-2019) State Key Laboratory of Plant Genomics, Institute of
            Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
            100101, China"
FEATURES             Location/Qualifiers
     source          1..7307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             448..513
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     protein_bind    514..538
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             541..3477
                     /gene="SMXL6"
                     /label="Protein SMAX1-LIKE 6"
                     /note="Protein SMAX1-LIKE 6 from Arabidopsis thaliana.
                     Accession#: Q9LML2"
     protein_bind    complement(3482..3506)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3771..3789)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3796..3812)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      3953..4408
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4486..4590
                     /label="AmpR promoter"
     CDS             4591..5448
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5622..6210
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6498..6519
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6534..6564
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6572..6588
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6596..6612
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6630..6648
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        6821..7307
                     /label="CaMV35S(long)"
                     /note="Cauliflower mosaic virus 35S promoter (long)"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcatggac tacaaagacc atgacggtga ttataaagat
      481 catgacatcg actacaagga tgacgatgac aagacaagtt tgtacaaaaa agcaggctta
      541 atgccgacgc cggtgactac ggcgagagaa tgcttgacgg aagaagctgc tcgtgccctc
      601 gacgatgctg ttgttgtagc tcgtcggaga agccacgcgc agacgacgtc tcttcatgca
      661 gtttctgctc ttttagctat gccgtcgtcg attctccgtg aagtttgcgt ctcacgcgcc
      721 gctaggagtg ttccttactc gtcgcgactt caattccgag ctcttgagct ctgcgtcggt
      781 gtatctctcg acaggcttcc gtcgtcgaag tctccggcga cggaagaaga tccaccggtt
      841 tcgaattcgc tcatggcggc gatcaaacgg tctcaggcga accagagacg gcatccggag
      901 tcgtatcatc ttcagcagat ccacgctagt aacaacggcg ggggaggatg ccagacgacg
      961 gttttgaaag tcgaattgaa gtatttcata ctctcgatcc ttgacgatcc gattgtgaat
     1021 cgggtattcg gagaagctgg gtttcggagc tccgaaatta agctcgatgt gcttcaccct
     1081 ccggtaacac aactttcttc ccgtttctct agaggtcgtt gtccgcctct cttcctctgt
     1141 aatcttccca actcagatcc gaatcgtgag ttcccgttta gtgggagcag tggtttcgat
     1201 gaaaattccc ggaggattgg agaagtatta ggcaggaaag ataagaagaa ccctctgctt
     1261 attggtaact gtgctaatga agctcttaaa acgttcacgg attcgatcaa cagtgggaag
     1321 ttagggtttc ttcagatgga tattagcgga ttaagcttga ttagtatcga gaaggagatt
     1381 agtgagattt tagccgatgg atcgaaaaac gaagaagaga ttcgaatgaa agtggatgac
     1441 ctaggaagaa ctgtagagca aagtggctcg aaatcgggga tagtgcttaa tctgggagag
     1501 ctcaaggttt tgaccagtga agccaatgcg gctcttgaga ttttggtgtc gaagctttcg
     1561 gatttgctga aacatgaaag taaacaactt tcgttcatcg gatgtgtatc gagtaatgag
     1621 acttacacga agcttattga tcggtttcct actatagaga aggattggga ccttcatgtt
     1681 cttccaatca cagcctctac taaaccttcg actcaagggg tttatcccaa atcgagcttg
     1741 atgggatcct ttgttccctt tggagggttc ttctcatcaa catcaaattt cagagttcca
     1801 ttgagtagca cagtgaatca gacgctctct agatgccacc tctgcaacga gaagtatttg
     1861 caagaagtag ccgctgttct caaggccggt tcgagtcttt ctctggctga caaatgttcc
     1921 gagaagttag ccccatggct acgggctatt gagaccaaag aagacaaggg aataacaggc
     1981 agcagtaagg ctttagatga cgctaataca tcagcctcgc aaaccgctgc tctacagaag
     2041 aaatgggaca acatatgcca aagtatccat cacactccgg cgtttcctaa acttggtttt
     2101 cagtcggtga gtccgcagtt cccagttcag actgagaaga gtgtgagaac tcctacaagc
     2161 tatttggaga cgcctaaact gctgaatccg ccaatctcaa agccaaaacc tatggaggat
     2221 cttacggcat cggtgactaa ccgcacagtg agtttgcctt tgagctgtgt tactacagat
     2281 tttgggttgg gagtaatcta tgcatccaaa aaccaggaat caaaaacaac gagggagaaa
     2341 ccgatgctgg tgactctaaa ctcttcttta gaacatacat atcagaaaga tttcaagtct
     2401 ctcagagaaa tactctctcg taaagttgcc tggcagaccg aagctgtaaa tgccataagc
     2461 caaattatct gcggatgcaa aaccgactcc acgcgaagaa accaagcaag cggaatttgg
     2521 ctggctcttc ttggacccga taaagtgggg aagaagaaag tggcgatgac tctttctgaa
     2581 gtcttctttg gtggtaaagt caattacata tgtgtagatt ttggggcaga gcattgttcc
     2641 cttgatgaca aattcagagg caaaacagtg gtggattacg taaccggtga gttatctagg
     2701 aaaccacact ctgttgtttt actcgaaaac gtggaaaaag ctgagttccc ggatcagatg
     2761 agattgtctg aagctgtgag tacggggaaa atccgtgatt tgcatggaag agtgattagt
     2821 atgaaaaatg tgattgttgt tgtgacgtct gggattgcca aggataatgc cactgaccat
     2881 gttattaaac ctgtgaagtt tcctgaggag caagttctca gcgcgagaag ctggaaactg
     2941 cagataaagc taggagatgc tactaaattt ggggtaaata agagaaaata tgagctagaa
     3001 acagcgcaac gtgcagtgaa ggtgcaacgt tcatatctgg atctgaatct tccagtgaat
     3061 gaaacagaat ttagccctga tcatgaggca gaggacaggg acgcttggtt cgatgaattc
     3121 attgaaaaag tagatggaaa agtgacgttc aaaccggttg atttcgatga gttagccaag
     3181 aacattcaag agaagattgg ttcacatttt gagcggtgct ttggatccga aacacatcta
     3241 gaacttgata aagaagtgat ccttcagatt ctggcggctt catggtcatc attatcatcg
     3301 ggcgaagaag aagggagaac aatagttgat cagtggatgc aaacagttct tgctcgaagc
     3361 tttgctgaag caaaacagaa gtacggttcg aatcccatgt tgggcgtgaa gctggttgct
     3421 tcttctagcg gcttagcttc cggagtagaa ttgccggcga aggtggatgt gatatggtga
     3481 tacccagctt tcttgtacaa agtggtgata tcccgcggcc atgctagagt ccgcaaaaat
     3541 caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa tgtgtgagta
     3601 gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg agcatataag
     3661 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
     3721 aaaaccaaaa tccagtgacc tgcaggcatg cgacgtcggg cccaattcgc cctatagtga
     3781 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     3961 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4021 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4081 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4141 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4201 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4261 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4321 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4381 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     4441 gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4501 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4561 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4621 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4681 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4741 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4801 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4861 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4921 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4981 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5041 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5101 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5161 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5221 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5281 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5341 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5401 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5461 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5521 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5581 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5641 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5701 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5761 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5821 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5881 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5941 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6001 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6061 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6121 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6181 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6241 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6301 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6361 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6421 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6481 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6541 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6601 aacagctatg accatgatta cgccaagcta tttaggtgac actatagaat actcaagcta
     6661 tgcatccaac gcgttgggag ctctcccata tggtcgacta gagccaagct gatctccttt
     6721 gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag aaagttcgac
     6781 ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct cttcaatttc
     6841 agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct catcaagacg
     6901 atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt taaagatgca
     6961 gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc
     7021 agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata
     7081 gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga gtcaaaaatt
     7141 cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat acagagtctt
     7201 ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cactctcgtc
     7261 tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctat
//
