pDXTP vector (V015393)

LOCUS       62056_8650        9939 bp DNA     circular SYN 26-JAN-2020
DEFINITION  Cloning vector pDXTP, complete sequence.
ACCESSION   MN044709
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Sterner ZR, Rankin SA, Wlizla M, Choi JA, Luedeke DM, Zorn AM, 
            Buchholz DR.
  TITLE     Novel vectors for functional interrogation of Xenopus ORFeome coding
            sequences
  JOURNAL   Genesis 57 (10), e23329 (2019)
  PUBMED    31403250
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   Buchholz DR.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2019) Biological Sciences, University of 
            Cincinnati, 312 Clifton Ct., Cincinnati, OH 45221, USA
REFERENCE   3  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis"; 
            date: "2019"; volume: "57"; issue: "10"; pages: "e23329"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUN-2019) Biological Sciences, University of Cincinnati, 312 
            Clifton Ct., Cincinnati, OH 45221, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label=attB
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et
                     al., 2000)"
     protein_bind    56..73
                     /label=I-SceI meganuclease binding site
                     /bound_moiety="I-SceI meganuclease"
     protein_bind    74..273
                     /label=tol2 binding site
                     /bound_moiety="tol2"
                     /note="tol2 5' site, the minimal 200bp"
     regulatory      274..919
                     /note="chicken HS4 extended (composed of HS4 core and HS4
                     3' 400 bp)"
                     /regulatory_class="insulator"
     protein_bind    931..978
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    1001..1034
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1089..1213
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1238..1268
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1322..1978
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2323..2625
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2669..2793)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2817..3560
                     /label=Tet-On(R) 3G
                     /note="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of 
                     doxycycline"
     regulatory      3578..4033
                     /label=SV40 polyadenylation sequence
                     /note="SV40 polyadenylation sequence"
                     /regulatory_class="other"
     regulatory      4034..4679
                     /note="chicken HS4 extended (composed of HS4 core and HS4
                     3' 400 bp)"
                     /regulatory_class="insulator"
     regulatory      4718..5031
                     /label=CRY promoter
                     /note="CRY promoter"
                     /regulatory_class="promoter"
     CDS             5032..5745
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
     polyA_signal    complement(5798..5932)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6025..6042
                     /label=I-SceI meganuclease binding site
                     /bound_moiety="I-SceI meganuclease"
     protein_bind    6109..6142
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    6165..6212
                     /label=flippase recombinase binding site
                     /bound_moiety="flippase recombinase"
                     /note="FRT3 site"
     regulatory      6224..6869
                     /note="chicken HS4 extended (composed of HS4 core and HS4
                     3' 400 bp)"
                     /regulatory_class="insulator"
     protein_bind    6870..7019
                     /label=tol2 binding site
                     /bound_moiety="tol2"
                     /note="tol2 3'site"
     protein_bind    complement(7020..7037)
                     /label=I-SceI meganuclease binding site
                     /bound_moiety="I-SceI meganuclease"
     promoter        complement(7064..7082)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7100..7116)
                     /label=M13R
                     /note="M13R"
     promoter        complement(7148..7178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7193..7214)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7502..8090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8264..9121)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9122..9226)
                     /label=AmpR promoter
     rep_origin      complement(9304..9759)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     9880..9896
                     /label=pUC/M13F
                     /note="pUC/M13F"
     promoter        9923..9939
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"