LOCUS       62056_8650        9939 bp DNA     circular SYN 26-JAN-2020
DEFINITION  Cloning vector pDXTP, complete sequence.
ACCESSION   MN044709
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Sterner ZR, Rankin SA, Wlizla M, Choi JA, Luedeke DM, Zorn AM, 
            Buchholz DR.
  TITLE     Novel vectors for functional interrogation of Xenopus ORFeome coding
            sequences
  JOURNAL   Genesis 57 (10), e23329 (2019)
  PUBMED    31403250
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   Buchholz DR.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2019) Biological Sciences, University of 
            Cincinnati, 312 Clifton Ct., Cincinnati, OH 45221, USA
REFERENCE   3  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis"; 
            date: "2019"; volume: "57"; issue: "10"; pages: "e23329"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUN-2019) Biological Sciences, University of Cincinnati, 312 
            Clifton Ct., Cincinnati, OH 45221, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label=attB
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et
                     al., 2000)"
     protein_bind    56..73
                     /label=I-SceI meganuclease binding site
                     /bound_moiety="I-SceI meganuclease"
     protein_bind    74..273
                     /label=tol2 binding site
                     /bound_moiety="tol2"
                     /note="tol2 5' site, the minimal 200bp"
     regulatory      274..919
                     /note="chicken HS4 extended (composed of HS4 core and HS4
                     3' 400 bp)"
                     /regulatory_class="insulator"
     protein_bind    931..978
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    1001..1034
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1089..1213
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1238..1268
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1322..1978
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2323..2625
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2669..2793)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2817..3560
                     /label=Tet-On(R) 3G
                     /note="modified rtTA protein that binds tightly to
                     promoters containing the tet operator in the presence of 
                     doxycycline"
     regulatory      3578..4033
                     /label=SV40 polyadenylation sequence
                     /note="SV40 polyadenylation sequence"
                     /regulatory_class="other"
     regulatory      4034..4679
                     /note="chicken HS4 extended (composed of HS4 core and HS4
                     3' 400 bp)"
                     /regulatory_class="insulator"
     regulatory      4718..5031
                     /label=CRY promoter
                     /note="CRY promoter"
                     /regulatory_class="promoter"
     CDS             5032..5745
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
     polyA_signal    complement(5798..5932)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6025..6042
                     /label=I-SceI meganuclease binding site
                     /bound_moiety="I-SceI meganuclease"
     protein_bind    6109..6142
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    6165..6212
                     /label=flippase recombinase binding site
                     /bound_moiety="flippase recombinase"
                     /note="FRT3 site"
     regulatory      6224..6869
                     /note="chicken HS4 extended (composed of HS4 core and HS4
                     3' 400 bp)"
                     /regulatory_class="insulator"
     protein_bind    6870..7019
                     /label=tol2 binding site
                     /bound_moiety="tol2"
                     /note="tol2 3'site"
     protein_bind    complement(7020..7037)
                     /label=I-SceI meganuclease binding site
                     /bound_moiety="I-SceI meganuclease"
     promoter        complement(7064..7082)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7100..7116)
                     /label=M13R
                     /note="M13R"
     promoter        complement(7148..7178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7193..7214)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7502..8090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8264..9121)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9122..9226)
                     /label=AmpR promoter
     rep_origin      complement(9304..9759)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     9880..9896
                     /label=pUC/M13F
                     /note="pUC/M13F"
     promoter        9923..9939
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgtgccaggg cgtgcccttg ggctccccgg gcgcgtaggg
       61 ataacagggt aatcagaggt gtaaagtact tgagtaattt tacttgatta ctgtacttaa
      121 gtattatttt tggggatttt tactttactt gagtacaatt aaaaatcaat acttttactt
      181 ttacttaatt acattttttt agaaaaaaaa gtacttttta ctccttacaa ttttatttac
      241 agtcaaaaag tacttatttt ttggagatca cttacgggga cagccccccc ccaaagcccc
      301 cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg
      361 ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca
      421 cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc
      481 agacacctgg ggggatacgg ggaaatgtgt ctgagcctgc atgtttgatg gtgtctggat
      541 gcaagcagaa ggggtggaag agcttgcctg gagagataca gctgggtcag taggactggg
      601 acaggcagct ggagaattgc catgtagatg ttcatacaat cgtcaaatca tgaaggctgg
      661 aaaagccctc caagatcccc aagaccaacc ccaacccacc caccgtgccc actggccatg
      721 tccctcagtg ccacatcccc acagttcttc atcacctcca gggacggtga cccccccacc
      781 tccgtgggca gctgtgccac tgcagcaccg ctctttggag aaggtaaatc ttgctaaatc
      841 cagcccgacc ctcccctggc acaacgtaag gccattatct ctcatccaac tccaggacgg
      901 agtcagtgag gatggggctc gtctcaagtg gaagttccta ttccgaagtt cctattctct
      961 agaaagtata ggaacttccg tctcaagtgg caagagttgc ataacttcgt ataatgtatg
     1021 ctatacgaag ttatgcaaga gttgcggcgc gccaccggtc ctgcaggccg gccgcgtcga
     1081 cccttaggac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
     1141 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     1201 tccagtcact atggctgccg cattaggcac cccaggcttt acactttatg cttccggctc
     1261 gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggaggta aggaagctaa
     1321 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     1381 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcaactgga
     1441 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1501 tcacattctt gcccgcctga tgaatgctca tccagagttc cgtatggcaa tgaaagacgg
     1561 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1621 aacattttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1681 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1741 gaatatgttt ttcgtgtcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1801 ggccaatatg gacaacttct tcgcccccgt tttcacaatg ggcaaatatt atacgcaagg
     1861 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1921 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1981 aaggtctgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     2041 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct
     2101 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     2161 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     2221 gtgccgaacg ctggaaagcg gaaaatcagg aagggatgga tgaggtcgcc cggtttattg
     2281 aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt taaggtttac
     2341 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     2401 acgcccgggc gacggatggt gatccccctg gccagtgctc gtctgctgtc agataaagtc
     2461 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgactacc
     2521 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     2581 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     2641 cttatacaca gccagtctgc aggtagacca tagtgactgg atatgttgtg ttttacagta
     2701 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     2761 cgtttctcgt tcagctttct tgtacaaagt ggttcctcag ccacgtcatc gagcttatgt
     2821 ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta
     2881 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     2941 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     3001 ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     3061 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     3121 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     3181 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     3241 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     3301 ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag
     3361 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     3421 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     3481 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     3541 accttgacat gctccccggg taactaagta aggatccaga catgataaga tacattgatg
     3601 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     3661 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     3721 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     3781 acctctacaa atgtggtatg gctgattatg atcctgcaag cctcgtcgtc ctggccggac
     3841 cacgctatct gtgcaaggtc cccggccccg gacgcgcgct ccatgagcag agcgcccgcc
     3901 gccgaggcga agactcgggc ggcgccctgc ccgtcccacc aggtcaacag gcggtaaccg
     3961 gcctcttcat cgggaatgcg cgcgaccttc agcatcgccg gcatgtcccc ctggcggacg
     4021 ggaagtatcc agcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc
     4081 tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc
     4141 cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat
     4201 cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg
     4261 ggaaatgtgt ctgagcctgc atgtttgatg gtgtctggat gcaagcagaa ggggtggaag
     4321 agcttgcctg gagagataca gctgggtcag taggactggg acaggcagct ggagaattgc
     4381 catgtagatg ttcatacaat cgtcaaatca tgaaggctgg aaaagccctc caagatcccc
     4441 aagaccaacc ccaacccacc caccgtgccc actggccatg tccctcagtg ccacatcccc
     4501 acagttcttc atcacctcca gggacggtga cccccccacc tccgtgggca gctgtgccac
     4561 tgcagcaccg ctctttggag aaggtaaatc ttgctaaatc cagcccgacc ctcccctggc
     4621 acaacgtaag gccattatct ctcatccaac tccaggacgg agtcagtgag gatggggctt
     4681 ttactcccta tcagtttaat taagaattca tttaaatgct gctaagcaat ggactggaaa
     4741 atttgtgaca ttgagttttt ggaataactg ggaatgctgg ataactcttt gggtaaagct
     4801 atggaacaaa agtaccagca tgaactaaga aatgactgtc acagcacaac atcaaacaca
     4861 atgcaagaca aaggttctct gcttttgcct tacttgacag aaaaagtaag ctctaggttc
     4921 atcgcatgca gacagcagca atacatgaca tatataaagg ctggctcttt tctcccttcc
     4981 attgaactga aacttccact cagtcagact tgcgggggat ccacagccac catgagtaaa
     5041 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
     5101 gggtacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
     5161 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
     5221 ctcacttatg gtgttcaatg cttttcaaga tatccagatc atatgaagcg gcacgacttc
     5281 ttcaagagcg ccatgcctga gggatacgtg caggagagga ccatcttctt caaggacgac
     5341 gggaactaca agacacgtgc tgaagtcaag tttgagggag acaccctcgt caacaggatc
     5401 gagcttaagg gaatcgattt caaggaggac ggaaacatcc tcggccacaa gttggaatac
     5461 aactacaact cccacaacgt atacatcatg gccgacaagc aaaagaacgg catcaaagcc
     5521 aacttcaaga cccgccacaa catcgaagac ggcggcgtgc aactcgctga tcattatcaa
     5581 caaaatactc caattggcga tgaccctgtc cttttaccag acaaccatta cctgtccaca
     5641 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
     5701 gtaacggctg ctgggattac acatggcatg gatgaactat acaaataact cgagcctcta
     5761 ggactagtcc tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat
     5821 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     5881 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     5941 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc
     6001 gcgcttggcg taatcatggt cataattacc ctgttatccc taacgcgctt ggcgtaatca
     6061 tggtcatagg cgatcgccct agggtttaaa cagatctgca gagactgcat aacttcgtat
     6121 aaagtatcct atacgaagtt atgcagagac tgccgtctcc ctgtgaagtt cctattccga
     6181 agttcctatt cttcaaatag tataggaact tccgtctccc tgtacgggga cagccccccc
     6241 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
     6301 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
     6361 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
     6421 ttgagcctgc agacacctgg ggggatacgg ggaaatgtgt ctgagcctgc atgtttgatg
     6481 gtgtctggat gcaagcagaa ggggtggaag agcttgcctg gagagataca gctgggtcag
     6541 taggactggg acaggcagct ggagaattgc catgtagatg ttcatacaat cgtcaaatca
     6601 tgaaggctgg aaaagccctc caagatcccc aagaccaacc ccaacccacc caccgtgccc
     6661 actggccatg tccctcagtg ccacatcccc acagttcttc atcacctcca gggacggtga
     6721 cccccccacc tccgtgggca gctgtgccac tgcagcaccg ctctttggag aaggtaaatc
     6781 ttgctaaatc cagcccgacc ctcccctggc acaacgtaag gccattatct ctcatccaac
     6841 tccaggacgg agtcagtgag gatggggcta atactcaagt acaattttaa tggagtactt
     6901 ttttactttt actcaagtaa gattctagcc agatactttt acttttaatt gagtaaaatt
     6961 ttccctaagt acttgtactt tcacttgagt aaaatttttg agtacttttt acacctctga
     7021 ttaccctgtt atccctaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
     7081 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7141 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7201 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7261 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7321 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7381 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7441 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7501 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7561 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7621 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7681 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7741 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7801 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7861 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7921 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7981 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8041 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8101 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8161 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8221 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8281 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8341 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8401 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8461 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8521 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8581 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8641 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8701 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8761 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8821 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8881 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8941 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9001 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9061 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9241 aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     9301 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     9361 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     9421 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     9481 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     9541 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     9601 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     9661 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     9721 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
     9781 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     9841 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     9901 taaaacgacg gccagtgaat tgtaatacga ctcactata
//
