pCIneo-3xFLAG-SLBP vector (V015365)

LOCUS       V015365                 6411 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V015365
VERSION     V015365
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 6411)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..6411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..380
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..592
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          753..885
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     promoter        930..948
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             957..1022
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     misc_feature    1071..1088
                     /label="linker"
                     /note="linker"
     CDS             1089..1898
                     /gene="SLBP"
                     /label="Histone RNA hairpin-binding protein"
                     /note="Histone RNA hairpin-binding protein from Homo
                     sapiens. Accession#: Q14493"
     protein_bind    complement(1900..1924)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    complement(1978..2099)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2285..2740
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2857..3214
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3265..4056
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4123..4171
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        4477..4581
                     /label="AmpR promoter"
     CDS             4582..5439
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5613..6201
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"