LOCUS       V015365                 6411 bp    DNA     circular SYN 28-OCT-2020
DEFINITION  Exported.
ACCESSION   V015365
VERSION     V015365
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Declercq M, Biquand E, Karim M, Pietrosemoli N, Jacob Y, Demeret C,
            Barbezange C, van der Werf S.
  TITLE     Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to
            promote viral transcription
  JOURNAL   Nucleic Acids Res 48 (18), 10428-10440 (2020)
   PUBMED   32960265
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Declercq M, Biquand E, Demeret C, Karim M, Pietrosemoli N, Jacob Y,
            Demeret C, Van der Werf S, Barbezange C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2020) National Influenza Centre, Sciensano, Rue
            Juliette Wytsman 14, Brussels 1180, Belgium
REFERENCE   3  (bases 1 to 6411)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res"; date: "2020"; volume: "48"; issue: "18"; pages:
            "10428-10440"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-SEP-2020) National Influenza Centre, Sciensano, Rue Juliette
            Wytsman 14, Brussels 1180, Belgium"
FEATURES             Location/Qualifiers
     source          1..6411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..380
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..592
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          753..885
                     /label="chimeric intron"
                     /note="chimera between introns from human beta-globin and
                     immunoglobulin heavy chain genes"
     promoter        930..948
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             957..1022
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     misc_feature    1071..1088
                     /label="linker"
                     /note="linker"
     CDS             1089..1898
                     /gene="SLBP"
                     /label="Histone RNA hairpin-binding protein"
                     /note="Histone RNA hairpin-binding protein from Homo
                     sapiens. Accession#: Q14493"
     protein_bind    complement(1900..1924)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    complement(1978..2099)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      2285..2740
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2857..3214
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3265..4056
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4123..4171
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     promoter        4477..4581
                     /label="AmpR promoter"
     CDS             4582..5439
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5613..6201
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctgcgatcgc ccgccccgtt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      601 gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa ttgctaacgc
      661 agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agtgactctc ttaaggtagc
      721 cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt
      781 aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
      841 cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt
      901 tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct agcatggact
      961 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     1021 agctcgagaa ttcacgcgtg gtacctctag agtcgaccca tcaaacaagt ttgtacaaaa
     1081 aagttggcat ggcctgccgc ccgcgaagcc cgccgaggca tcagagccgc tgcgacggtg
     1141 acgccagccc gccgtccccc gcgcgatgga gcctgggacg gaagcgcaga gccgacggca
     1201 ggcgctggag gcccgaagac gccgaggagg cagagcaccg cggcgccgag cgcagacccg
     1261 agagctttac cactcctgaa ggccctaaac cccgttccag atgctctgac tgggcaagtg
     1321 cagttgaaga agatgaaatg aggaccagag ttaacaaaga aatggcaaga tataaaagga
     1381 aactcctcat caatgacttt ggaagagaga gaaaatcatc atcaggaagt tctgattcaa
     1441 aggagtctat gtctactgtg ccggctgact ttgagacaga tgaaagtgtc ctaatgagga
     1501 gacagaagca gatcaactat gggaagaaca caattgccta cgatcgttat attaaagaag
     1561 tcccaagaca ccttcgacaa cctggcattc atcccaagac ccctaataaa tttaagaagt
     1621 atagtcgacg ttcatgggac cagcaaatca aactctggaa ggtggctctg catttttggg
     1681 atcctccagc ggaagaagga tgtgatttgc aagaaataca ccctgtagac cttgaatctg
     1741 cagaaagcag ctccgagccc cagaccagct ctcaggatga ctttgatgtg tactctggca
     1801 cacccaccaa ggtgagacac atggacagtc aagtggagga tgagtttgat ttggaagctt
     1861 gtttaactga acccttgaga gacttctcag ccatgagcta cccaactttc ttgtacaaag
     1921 tggttcgatg ggcggccgct tccctttagt gagggttaat gcttcgagca gacatgataa
     1981 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     2041 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     2101 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     2161 aaagcaagta aaacctctac aaatgtggta aaatccgata aggatcgatc cgggctggcg
     2221 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2281 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2341 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2401 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2461 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2521 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2581 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2641 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2701 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     2761 ttacgcatct gtgcggtatt tcacaccgca tacgcggatc tgcgcagcac catggcctga
     2821 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg
     2881 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2941 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3001 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3061 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3121 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3181 aggaggcttt tttggaggcc taggcttttg caaaaagctt gattcttctg acacaacagt
     3241 ctcgaactta aggctagagc caccatgatt gaacaagatg gattgcacgc aggttctccg
     3301 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     3361 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     3421 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     3481 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     3541 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     3601 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     3661 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     3721 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     3781 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     3841 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     3901 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     3961 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4021 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     4081 tgaccgacca agcgacgccc aacctgccat cacgatggcc gcaataaaat atctttattt
     4141 tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat tcgcgtatgg
     4201 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     4261 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     4321 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4381 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4441 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4501 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4561 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4621 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4681 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4741 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4801 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4861 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4921 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4981 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5041 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5101 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5161 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5221 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5281 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5341 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5401 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5461 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5521 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5581 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5641 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5701 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5761 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5821 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5881 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5941 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6001 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6061 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6121 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6181 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6241 tgctggcctt ttgctcacat ggctcgacag atcttcaata ttggccatta gccatattat
     6301 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     6361 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt t
//
