pMEMORY8190 vector (V015360)

MSDS

Basic Vector Information

Vector Name:: pMEMORY8190

Antibiotic Resistance:: Ampicillin

Length:: 3958 bp

Type:: Gateway destination vector

Replication origin:: ori

Source/Author:: Fukuda E, Mori M, Shiku H, Miyahara Y

pMEMORY8190 vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMEMORY8190 vector Sequence

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Download GeneBank File(.gb)

LOCUS       62056_16740        3958 bp DNA     circular SYN 06-SEP-2019
DEFINITION  Gateway destination vector pMEMORY8190 DNA, complete sequence.
ACCESSION   LC498629
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Fukuda E, Mori M, Shiku H, Miyahara Y, Kawamura Y, Ogawa K, Goshima 
            N.
  TITLE     Development of novel INSOL-tag enabling proteome-wide protein 
            handling and application to protein array
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Fukuda E, Goshima N.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2019) Contact:Eriko Fukuda National Institute of 
            Advanced Industrial Science and Technology, Molecular Profiling 
            Research Center for Drug Discovery; 2-4-7 Aomi, Koto-ku, Tokyo 
            135-0064, Japan
REFERENCE   3  (bases 1 to 3958)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2019) Contact:Eriko Fukuda National Institute of Advanced 
            Industrial Science and Technology, Molecular Profiling Research 
            Center for Drug Discovery; 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, 
            Japan"
FEATURES             Location/Qualifiers
     source          1..3958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        40..58
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      183..193
                     /label=translation terminator
                     /note="translation terminator"
                     /regulatory_class="terminator"
     misc_feature    203..247
                     /label=mKG2-5C
                     /note="mKG2-5C"
     protein_bind    305..429
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1662..1786)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1806..1840
                     /label=translation terminator
                     /note="translation terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(2073..2661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2907..2940
                     /label=polyA
                     /note="polyA"
     CDS             complement(3013..3870)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3871..3941)
                     /label=AmpR promoter

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