LOCUS       62056_16740        3958 bp DNA     circular SYN 06-SEP-2019
DEFINITION  Gateway destination vector pMEMORY8190 DNA, complete sequence.
ACCESSION   LC498629
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Fukuda E, Mori M, Shiku H, Miyahara Y, Kawamura Y, Ogawa K, Goshima 
            N.
  TITLE     Development of novel INSOL-tag enabling proteome-wide protein 
            handling and application to protein array
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Fukuda E, Goshima N.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2019) Contact:Eriko Fukuda National Institute of 
            Advanced Industrial Science and Technology, Molecular Profiling 
            Research Center for Drug Discovery; 2-4-7 Aomi, Koto-ku, Tokyo 
            135-0064, Japan
REFERENCE   3  (bases 1 to 3958)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2019) Contact:Eriko Fukuda National Institute of Advanced 
            Industrial Science and Technology, Molecular Profiling Research 
            Center for Drug Discovery; 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, 
            Japan"
FEATURES             Location/Qualifiers
     source          1..3958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        40..58
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      183..193
                     /label=translation terminator
                     /note="translation terminator"
                     /regulatory_class="terminator"
     misc_feature    203..247
                     /label=mKG2-5C
                     /note="mKG2-5C"
     protein_bind    305..429
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1662..1786)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1806..1840
                     /label=translation terminator
                     /note="translation terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(2073..2661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2907..2940
                     /label=polyA
                     /note="polyA"
     CDS             complement(3013..3870)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3871..3941)
                     /label=AmpR promoter
ORIGIN
        1 cccgggtgtc gttagaacgc ggctacgcta gcacatacga tttaggtgac actatagaac
       61 cgcgggtttt tatttttaat tttctttcaa atacttctag ctagagtatt tttacaacaa
      121 ttaccaacaa caacaacaaa caacaacaac attacatttt acattctaca actacaagat
      181 cttgattgag tgactcgaga ccatgggcaa catcaccgag ctggtggagg acgccgtggc
      241 ccactcccaa ttgggtggag gcggttcagg cggaggtggc tctggcggtg gcggatcggg
      301 taccacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
      361 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
      421 gtcactatgg cggccgcgat atccccagct ctgcccatgc acatgcaata ctcaaagatg
      481 cacaggattc cccaggtgtg gttctgggtc agggagtcat cagggctggc aagggctggg
      541 agaatggacc agaaagccat agagtgagat acagggggaa gaagaacaca gactggcaca
      601 ccagatcccc ggccctcacc ttcacggtcc tttggccaag ctgccccctg gttccaatag
      661 ttctgggact aaaccctggc ttttgccctt gcgccaggag tagtggtgaa tgtaatcatt
      721 ctggccacat cattgaggga actagatggg atcagggcca gctttgcctc ctgttcactt
      781 tgtccagagc tgacatcaga gcctgctgga gagtaggtga gcagcaatat gtgtcccttt
      841 ttacccatgt ccagatgcag catccatcct tacgacctca ttccctccca gcgatatctt
      901 cgaacccgta accgaagata tcttctatcc tattataatg gctatgccca ttcagaaagc
      961 aaaacaaaat ttgtttggac aaaacagaaa tttttccaat ggccatgtag tttgtgaaaa
     1021 aaagcagcag ctggagttgg attctattgt agaagaaacc ataacaggag attatgcctt
     1081 aatcataaat ggccacagtt tggctcatgc cctagaaagt gatgtcaaga atgatctcct
     1141 agaacttgct tgcatgtgta agactgtaat ttgctgcagg gtcactccac tccagaaagc
     1201 ccaagtggta gagctggtga agaagtacag aaatgctgtt actttggcca ttggtgatgg
     1261 agccaatgat gtcagcatga ttaaaagtgc tcacattggt gttggcatca gcggccagga
     1321 aggattgcaa gcagtcttag ccagcgacta ttcatttgca cagtttagat atctccaaag
     1381 gcttctcctt gttcatggaa ggtggtctta tttccgaatg tgcaaattct tatgctattt
     1441 cttctataag aattttgcat ttacacttgt gcatttctgg tttggtttct tctgtggttt
     1501 ctcagcccag gttggtaaaa gcatgttttt ttttttgttt gtttgtttgt ttttgtatga
     1561 tattacaaat gactcttctt catagctctt tactaataaa ttcagagcat tttttaatgg
     1621 ctgattcaag taatagtatt acaattatgg atatcactag tcatagtgac tggatatgtt
     1681 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     1741 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtggtc gacgtatacg
     1801 catgctgatt gagtgagtag ttagatagat aagtaagtaa cttaagggat cccgactggc
     1861 gagagccagg taacgaatgg atccacgcgt tcttccgctt cctcgctcac tgactcgctg
     1921 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1981 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2041 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2101 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2161 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2221 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2281 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2341 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2401 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2461 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2521 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2581 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2641 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactctaga atgcataggt
     2701 gctgaaatat aaagtttgtg tttctaaaac acacgtggta cgtacgataa cgtacagtgt
     2761 ttttccctcc acttaaatcg aagggtagtg tcttggagcg cgcggagtaa acatatatgg
     2821 ttcatatatg tccgtaggca cgtaaaaaaa gcgagggatt cgaattcccc cggaaccccc
     2881 ggttggggcc cacgcctcga tcgagcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2941 ggcgcgccgc cctatctacc ttgcgtaggt agggttcttt tgttcttttc tacggggtct
     3001 gaccggccgt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3061 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3121 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3181 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3241 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3301 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3361 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3421 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3481 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3541 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3601 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3661 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3721 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3781 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3841 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt atgaagcatt
     3901 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaac
//
